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Qualitative and quantitative analyses of Epimedium wushanense by high-performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry
Li, Hui-fang1; Guan, Xiang-yu1; Ye, Min1; Xiang, Cheng1; Lin, Chang-hu; Sun, Chao2; Guo, De-an1
关键词Epimedium wushanense HPLC-DAD-ESI-MS(n) Prenylated flavonoids Quality control
刊名JOURNAL OF SEPARATION SCIENCE
2011-06-01
DOI10.1002/jssc.201000899
34期:12页:1437-1446
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]FLAVONOL GLYCOSIDES ; PRIMARY OSTEOBLASTS ; CONSTITUENTS ; KOREANUM ; ICARIIN ; L.
英文摘要

A new HPLC-DAD-ESI-MS(n) method was developed for rapid separation, characterization and quantitation of flavonoids in Epimedium wushanense, a popular Chinese herbal medicine. For qualitative identification, a total of 37 compounds were characterized from the underground and aerial parts of E. wushanense. Among them, 28 compounds were prenylated flavonoids, and 23 were confirmed by comparing with reference standards. For quantitative analysis, 12 major flavonoids including kaempferol glycosides, desmethylicaritin glycosides, and icaritin glycosides were simultaneously determined by HPLC/UV. Samples were separated on a Waters Symmetry C(18) column at 35 degrees C eluted with a gradient three-component mobile phase of acetonitrile, methanol, and water containing 0.03% v/v formic acid. All the flavonoids showed good linearity (r(2) >= 0.9997). The recoveries varied from 92.6 to 106.1% at three concentration levels. This method was applied to the determination of 20 samples of different geographical sources, harvesting time, and plant parts. Contents of the predominant flavonoid, epimedin C, ranged from 1.4 to 5.1% in aerial parts and 1.0 to 2.8% in underground parts. The methods established in this paper were simple and reliable and could be used for the quality control of E. wushanense.

语种英语
WOS记录号WOS:000291986600010
项目编号985-2-119-121 ; 2009ZX09304-002
资助机构Peking University Health Science Center ; National Science &amp ; Technology Major Project of China
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61823
专题北京大学药学院
北京大学药学院_天然药物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Guizhou Acad Sci, Inst Biol, Guiyang, Peoples R China
推荐引用方式
GB/T 7714
Li, Hui-fang,Guan, Xiang-yu,Ye, Min,et al. Qualitative and quantitative analyses of Epimedium wushanense by high-performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(12):1437-1446.
APA Li, Hui-fang.,Guan, Xiang-yu.,Ye, Min.,Xiang, Cheng.,Lin, Chang-hu.,...&Guo, De-an.(2011).Qualitative and quantitative analyses of Epimedium wushanense by high-performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry.JOURNAL OF SEPARATION SCIENCE,34(12),1437-1446.
MLA Li, Hui-fang,et al."Qualitative and quantitative analyses of Epimedium wushanense by high-performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry".JOURNAL OF SEPARATION SCIENCE 34.12(2011):1437-1446.
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