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学科主题: 药学
题名:
Qualitative and quantitative analyses of Epimedium wushanense by high-performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry
作者: Li, Hui-fang1; Guan, Xiang-yu1; Ye, Min1; Xiang, Cheng1; Lin, Chang-hu; Sun, Chao2; Guo, De-an1
关键词: Epimedium wushanense ; HPLC-DAD-ESI-MS(n) ; Prenylated flavonoids ; Quality control
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2011-06-01
DOI: 10.1002/jssc.201000899
卷: 34, 期:12, 页:1437-1446
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: FLAVONOL GLYCOSIDES ; PRIMARY OSTEOBLASTS ; CONSTITUENTS ; KOREANUM ; ICARIIN ; L.
英文摘要:

A new HPLC-DAD-ESI-MS(n) method was developed for rapid separation, characterization and quantitation of flavonoids in Epimedium wushanense, a popular Chinese herbal medicine. For qualitative identification, a total of 37 compounds were characterized from the underground and aerial parts of E. wushanense. Among them, 28 compounds were prenylated flavonoids, and 23 were confirmed by comparing with reference standards. For quantitative analysis, 12 major flavonoids including kaempferol glycosides, desmethylicaritin glycosides, and icaritin glycosides were simultaneously determined by HPLC/UV. Samples were separated on a Waters Symmetry C(18) column at 35 degrees C eluted with a gradient three-component mobile phase of acetonitrile, methanol, and water containing 0.03% v/v formic acid. All the flavonoids showed good linearity (r(2) >= 0.9997). The recoveries varied from 92.6 to 106.1% at three concentration levels. This method was applied to the determination of 20 samples of different geographical sources, harvesting time, and plant parts. Contents of the predominant flavonoid, epimedin C, ranged from 1.4 to 5.1% in aerial parts and 1.0 to 2.8% in underground parts. The methods established in this paper were simple and reliable and could be used for the quality control of E. wushanense.

语种: 英语
所属项目编号: 985-2-119-121 ; 2009ZX09304-002
项目资助者: Peking University Health Science Center ; National Science &amp ; Technology Major Project of China
WOS记录号: WOS:000291986600010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61823
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Guizhou Acad Sci, Inst Biol, Guiyang, Peoples R China

Recommended Citation:
Li, Hui-fang,Guan, Xiang-yu,Ye, Min,et al. Qualitative and quantitative analyses of Epimedium wushanense by high-performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(12):1437-1446.
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