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学科主题: 药学
题名:
Qualitative and quantitative evaluation of Microctis Folium (the leaves of Microcos paniculata L.) by high performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry
作者: Hu, Kua1; Zhang, Jia-yu2; Zan, Ke1; Li, Jun1; Tu, Peng-fei1
刊名: ANALYTICAL METHODS
发表日期: 2014-09-07
DOI: 10.1039/c4ay00196f
卷: 6, 期:17, 页:6850-6859
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
研究领域[WOS]: Chemistry ; Food Science & Technology ; Spectroscopy
关键词[WOS]: DAD-ESI-MSN ; POLYMETHOXYLATED FLAVONOIDS ; IDENTIFICATION ; PLASMA
英文摘要:

Microctis Folium (MF), the leaves of Microcos paniculata L., which is widely used as a traditional Chinese medicine and a healthy tea, show various biological activities including antioxidant, cardiovascular risk, and so on. However, few studies on the quality control for this herbal medicine have been reported. In this paper, a reliable and sensitive online high-performance liquid chromatography coupled with diode array detection and electrospray ionization tandem ion trap mass spectrometry (HPLC-DAD-ESI-MS/MS) method for qualitative and quantitative evaluation of the characteristic chemical constituents of MF was developed. 32 compounds including 10 flavans, 16 flavonoid glycosides, 5 lignan glycosides and 1 megastigmadien glycoside were identified from MF, among which lignan glycosides and megastigmadien glycoside were first reported from the genus Microcos. Besides, based on HPLC-DAD-ESI-MS/MS data, a standardized HPLC fingerprint and a quantitative method were established by analyzing 13 batches of commercial herbal samples by HPLC with ultraviolet-visible (UV) detection method. Meanwhile, three major bioactive constituents including kaempferol-3-O-beta-D-glucoside, narcissi and isorhamnetin-3-O-beta-D-glucoside in MF were simultaneously determined with the same method, which could differentiate good quality batches from counterfeits. The results revealed that this HPLC-DAD-ESI-MS/MS analytical method established could be adopted as the comprehensive quality control method of MF.

语种: 英语
所属项目编号: 2009ZX09308-004 ; 2009ZX09311-004
项目资助者: Research Platform for Quality Standard of TCMs and Information System Building ; National S&amp ; T Major Project-Created Major New drugs Projects
WOS记录号: WOS:000340981600031
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61825
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Beijing Univ Chinese Med, Ctr Sci Expt, Beijing 100029, Peoples R China

Recommended Citation:
Hu, Kua,Zhang, Jia-yu,Zan, Ke,et al. Qualitative and quantitative evaluation of Microctis Folium (the leaves of Microcos paniculata L.) by high performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry[J]. ANALYTICAL METHODS,2014,6(17):6850-6859.
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