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Qualitative and quantitative evaluation of Microctis Folium (the leaves of Microcos paniculata L.) by high performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry
Hu, Kua1; Zhang, Jia-yu2; Zan, Ke1; Li, Jun1; Tu, Peng-fei1
刊名ANALYTICAL METHODS
2014-09-07
DOI10.1039/c4ay00196f
6期:17页:6850-6859
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
研究领域[WOS]Chemistry ; Food Science & Technology ; Spectroscopy
关键词[WOS]DAD-ESI-MSN ; POLYMETHOXYLATED FLAVONOIDS ; IDENTIFICATION ; PLASMA
英文摘要

Microctis Folium (MF), the leaves of Microcos paniculata L., which is widely used as a traditional Chinese medicine and a healthy tea, show various biological activities including antioxidant, cardiovascular risk, and so on. However, few studies on the quality control for this herbal medicine have been reported. In this paper, a reliable and sensitive online high-performance liquid chromatography coupled with diode array detection and electrospray ionization tandem ion trap mass spectrometry (HPLC-DAD-ESI-MS/MS) method for qualitative and quantitative evaluation of the characteristic chemical constituents of MF was developed. 32 compounds including 10 flavans, 16 flavonoid glycosides, 5 lignan glycosides and 1 megastigmadien glycoside were identified from MF, among which lignan glycosides and megastigmadien glycoside were first reported from the genus Microcos. Besides, based on HPLC-DAD-ESI-MS/MS data, a standardized HPLC fingerprint and a quantitative method were established by analyzing 13 batches of commercial herbal samples by HPLC with ultraviolet-visible (UV) detection method. Meanwhile, three major bioactive constituents including kaempferol-3-O-beta-D-glucoside, narcissi and isorhamnetin-3-O-beta-D-glucoside in MF were simultaneously determined with the same method, which could differentiate good quality batches from counterfeits. The results revealed that this HPLC-DAD-ESI-MS/MS analytical method established could be adopted as the comprehensive quality control method of MF.

语种英语
WOS记录号WOS:000340981600031
项目编号2009ZX09308-004 ; 2009ZX09311-004
资助机构Research Platform for Quality Standard of TCMs and Information System Building ; National S&amp ; T Major Project-Created Major New drugs Projects
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61825
专题北京大学药学院
北京大学药学院_天然药物学系
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Beijing Univ Chinese Med, Ctr Sci Expt, Beijing 100029, Peoples R China
推荐引用方式
GB/T 7714
Hu, Kua,Zhang, Jia-yu,Zan, Ke,et al. Qualitative and quantitative evaluation of Microctis Folium (the leaves of Microcos paniculata L.) by high performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry[J]. ANALYTICAL METHODS,2014,6(17):6850-6859.
APA Hu, Kua,Zhang, Jia-yu,Zan, Ke,Li, Jun,&Tu, Peng-fei.(2014).Qualitative and quantitative evaluation of Microctis Folium (the leaves of Microcos paniculata L.) by high performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry.ANALYTICAL METHODS,6(17),6850-6859.
MLA Hu, Kua,et al."Qualitative and quantitative evaluation of Microctis Folium (the leaves of Microcos paniculata L.) by high performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry".ANALYTICAL METHODS 6.17(2014):6850-6859.
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