IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Evaluating biotoxicity with fibroblasts derived from human embryonic stem cells
Wang, Xiaoying1; Li, Shenglin1,2; Cao, Tong3; Fu, Xin3; Yu, Guangyan4
关键词Human embryonic stem cell Fibroblast Biomaterial Cytotoxicity Genotoxicity
刊名TOXICOLOGY IN VITRO
2012-09-01
DOI10.1016/j.tiv.2012.04.002
26期:6页:1056-1063
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Toxicology
研究领域[WOS]Toxicology
关键词[WOS]IN-VITRO ; OXIDATIVE STRESS ; HUMAN BLASTOCYSTS ; SODIUM-FLUORIDE ; FEEDER CELLS ; CROSS-LINKS ; DNA-DAMAGE ; LINES ; ASSAY ; TOXICITY
英文摘要

To investigate the use of differentiated fibroblasts from human embryonic stem cells as a cellular model for cytotoxicity and genotoxicity screening. The EBf-H9 cells were derived from human embryonic stem cells (H9) via embryonic body (ES) and treated with Sodium fluoride (NaF) and Formaldehyde (FA). Proliferation, specific gene and protein expression and karyotype of cells were analyzed by MIT assay, RT-PCR, immunocytochemistry and karyotype analysis, respectively. Cytotoxicity was detected by MIT assay and flow cytometry, and genotoxicity was studied by micronucleus test (MNT), sister chromatid exchange (SCE) and comet assay. EBf-119s were spindle-shaped with a diploid karyotype. They expressed the fibroblast markers prolyl 4-hydroxylase beta and vimentin but did not express Oct-4 and Sox-2, and decreased expression of Nanog. The proliferation of EBf-H9 and murine L929 cells was inhibited by sodium fluoride (NaF) and formaldehyde (FA), and the cell cycle was arrested in different phases with the treatments. In genotoxicity assays with NaF and FA, positive responses were detected in human EBf-H9s comparable to those in the murine L929 cell line. EBf-H9 may be a suitable new cell source for toxicity research on biomaterials and other agents. (C) 2012 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000307323900037
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61828
专题北京大学口腔医学院
北京大学第一临床医学院_医学影像科
北京大学口腔医学院_外科
作者单位1.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Lab Oral & Maxillofacial Surg, Beijing, Peoples R China
3.Natl Univ Singapore, Dept Oral & Maxillofacial Surg, Singapore 117548, Singapore
4.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Wang, Xiaoying,Li, Shenglin,Cao, Tong,et al. Evaluating biotoxicity with fibroblasts derived from human embryonic stem cells[J]. TOXICOLOGY IN VITRO,2012,26(6):1056-1063.
APA Wang, Xiaoying,Li, Shenglin,Cao, Tong,Fu, Xin,&Yu, Guangyan.(2012).Evaluating biotoxicity with fibroblasts derived from human embryonic stem cells.TOXICOLOGY IN VITRO,26(6),1056-1063.
MLA Wang, Xiaoying,et al."Evaluating biotoxicity with fibroblasts derived from human embryonic stem cells".TOXICOLOGY IN VITRO 26.6(2012):1056-1063.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Xiaoying]的文章
[Li, Shenglin]的文章
[Cao, Tong]的文章
百度学术
百度学术中相似的文章
[Wang, Xiaoying]的文章
[Li, Shenglin]的文章
[Cao, Tong]的文章
必应学术
必应学术中相似的文章
[Wang, Xiaoying]的文章
[Li, Shenglin]的文章
[Cao, Tong]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。