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学科主题: 口腔医学
题名:
Evaluating biotoxicity with fibroblasts derived from human embryonic stem cells
作者: Wang, Xiaoying1; Li, Shenglin1,2; Cao, Tong3; Fu, Xin3; Yu, Guangyan4
关键词: Human embryonic stem cell ; Fibroblast ; Biomaterial ; Cytotoxicity ; Genotoxicity
刊名: TOXICOLOGY IN VITRO
发表日期: 2012-09-01
DOI: 10.1016/j.tiv.2012.04.002
卷: 26, 期:6, 页:1056-1063
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: IN-VITRO ; OXIDATIVE STRESS ; HUMAN BLASTOCYSTS ; SODIUM-FLUORIDE ; FEEDER CELLS ; CROSS-LINKS ; DNA-DAMAGE ; LINES ; ASSAY ; TOXICITY
英文摘要:

To investigate the use of differentiated fibroblasts from human embryonic stem cells as a cellular model for cytotoxicity and genotoxicity screening. The EBf-H9 cells were derived from human embryonic stem cells (H9) via embryonic body (ES) and treated with Sodium fluoride (NaF) and Formaldehyde (FA). Proliferation, specific gene and protein expression and karyotype of cells were analyzed by MIT assay, RT-PCR, immunocytochemistry and karyotype analysis, respectively. Cytotoxicity was detected by MIT assay and flow cytometry, and genotoxicity was studied by micronucleus test (MNT), sister chromatid exchange (SCE) and comet assay. EBf-119s were spindle-shaped with a diploid karyotype. They expressed the fibroblast markers prolyl 4-hydroxylase beta and vimentin but did not express Oct-4 and Sox-2, and decreased expression of Nanog. The proliferation of EBf-H9 and murine L929 cells was inhibited by sodium fluoride (NaF) and formaldehyde (FA), and the cell cycle was arrested in different phases with the treatments. In genotoxicity assays with NaF and FA, positive responses were detected in human EBf-H9s comparable to those in the murine L929 cell line. EBf-H9 may be a suitable new cell source for toxicity research on biomaterials and other agents. (C) 2012 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 2008DFB30090 ; 2011DFA32190
项目资助者: Ministry of Science and Technology of China (ISTCP)
WOS记录号: WOS:000307323900037
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61828
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Lab Oral & Maxillofacial Surg, Beijing, Peoples R China
3.Natl Univ Singapore, Dept Oral & Maxillofacial Surg, Singapore 117548, Singapore
4.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing, Peoples R China

Recommended Citation:
Wang, Xiaoying,Li, Shenglin,Cao, Tong,et al. Evaluating biotoxicity with fibroblasts derived from human embryonic stem cells[J]. TOXICOLOGY IN VITRO,2012,26(6):1056-1063.
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