|The role of hypertension-related gene in aortic vascular smooth muscle cells from mice and rats|
|Chen, GH; Liu, NK; Zhou, AR; Tang, CS; Ma, DL; Tang, J|
|关键词||HRG-1 gene cardiovascular diseases|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
Objective To study the role of hypertension-related gene (HRG-1) in cardiovascular disease.
Methods The expression of HRG-1 was analyzed with RT-PCR and Northern blotting. Vascular smooth muscle cell (VSMC) proliferation was measured with H-3-TdR incorporation and was confirmed with histological analysis.
Results Northern blot analysis showed that HRG-1 mRNA was expressed not only in VSMC, but also in various rat tissues (heart, brain, lung, kidney, and liver). In addition, the expression of HRG-1 mRNA in heart, brain, kidney and liver of spontaneously hypertensive rat (SHR) was lower than that in the same tissues of Wistar-Kyotorat (WKY). Semi-quantitative RT-PCR and histological analysis showed that the expression of HRG-1 mRNA in ApoE-knockout mice and in animal models of restenosis was decreased and neointimal formation was observed in both models. ET, All, and IL-1 stimulating VSMC proliferation reduced the expression of HRG-1 mRNA of VSMC. Atrial natriuretic factor (ANF), calcitonin gene-related peptide and adrenomedullin, inhibited VSMC proliferation and elevated the expression of HRG-1 mRNA. These effects could be blocked or attenuated by their corresponding antagonists or antibodies.
Conclusion HRG-1 is a gene related to VSMC proliferation. It may play an important role in several occlusive cardiovascular diseases including atherosclerosis, restenosis and hypertension.
|作者单位||Beijing Med Univ, Cardiovasc Res Inst, Beijing 100083, Peoples R China|
|Chen, GH,Liu, NK,Zhou, AR,et al. The role of hypertension-related gene in aortic vascular smooth muscle cells from mice and rats[J]. CHINESE MEDICAL JOURNAL,2001,114(8):833-836.|
|APA||Chen, GH,Liu, NK,Zhou, AR,Tang, CS,Ma, DL,&Tang, J.(2001).The role of hypertension-related gene in aortic vascular smooth muscle cells from mice and rats.CHINESE MEDICAL JOURNAL,114(8),833-836.|
|MLA||Chen, GH,et al."The role of hypertension-related gene in aortic vascular smooth muscle cells from mice and rats".CHINESE MEDICAL JOURNAL 114.8(2001):833-836.|
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