IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
Distinct actions of intermittent and sustained beta-adrenoceptor stimulation on cardiac remodeling
Ma XiaoWei1,3; Song Yao1,3; Chen Chao1,3; Fu YongNan1,3; Shen Qiang2,3; Li ZiJian1,3; Zhang YouYi1,3
关键词beta-adrenoceptor reactive oxygen species NADPH oxidase cardiac hypertrophy cardiac fibrosis
刊名SCIENCE CHINA-LIFE SCIENCES
2011-06-01
DOI10.1007/s11427-011-4183-9
54期:6页:493-501
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biology
研究领域[WOS]Life Sciences & Biomedicine - Other Topics
关键词[WOS]RAT VENTRICULAR MYOCYTES ; HEART-FAILURE ; OXIDATIVE STRESS ; IN-VIVO ; ALPHA(1)-ADRENERGIC RECEPTOR ; BLOOD-PRESSURE ; S6 KINASE ; HYPERTROPHY ; ISOPROTERENOL ; ACTIVATION
英文摘要

Heart disease is associated with increased sympathetic nerve activity and elevated levels of circulating catecholamines, resulting in chronic stimulation of the beta-adrenergic receptors (beta-AR) and consequent pathological cardiac remodeling. Experimentally, chronic administration of the beta-AR agonist isoproterenol (ISO) has been most commonly used to model beta-AR-induced cardiac remodeling. However, it remains unclear whether beta-AR-mediated cardiac remodeling and dysfunction differs between sustained versus pulsatile (intermittent) exposure to a beta-agonist. Here, we compare the effects of intermittent versus sustained administration of ISO on cardiac remodeling and function in mice. Animals were administered 5 mg (kg d)(-1) ISO for 2 weeks either by daily subcutaneous injection, or continuous infusion via an implanted osmotic minipump. Cardiac function and remodeling were determined by echocardiography, micromanometry and histology. Moreover, Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) were utilized to define the proteins and genes involved. Both sustained and intermittent administration of ISO resulted in a similar degree of cardiac hypertrophy (16% and 19%, respectively). However, mice receiving ISO by daily injection developed more severe ventricular systolic and diastolic dysfunction and myocardial fibrosis compared with mice receiving ISO via the osmotic minipump. The disparity in results between the delivery methods is suggested to be due, at least in part, to increased expression of fibrogenic factors, including connective tissue growth factor (CTGF) and NADPH oxidase (NOX4), in mice receiving intermittent application of ISO. In summary, compared with sustained exposure to a beta-AR agonist, intermittent beta-AR stimulation leads to more severe cardiac dysfunction and fibrosis. These findings not only further our understanding of beta-AR function in the setting of cardiac pathophysiology, but also highlight that significant differences can result dependent upon the mode of experimental beta-AR stimulation in inducing cardiomyopathy.

语种英语
WOS记录号WOS:000292050500001
项目编号2011CB503903 ; 81030001 ; 30971161 ; 30910103902
资助机构National Basic Research Program of China ; National Natural Science Foundation of China ; International Cooperation and Exchange of the National Natural Science Foundation of China
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61856
专题北京大学第三临床医学院_心血管内科
北京大学基础医学院
北京大学精神卫生研究所_精神科
作者单位1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100191, Peoples R China
3.Peking Univ, Inst Cardiovasc Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Ma XiaoWei,Song Yao,Chen Chao,et al. Distinct actions of intermittent and sustained beta-adrenoceptor stimulation on cardiac remodeling[J]. SCIENCE CHINA-LIFE SCIENCES,2011,54(6):493-501.
APA Ma XiaoWei.,Song Yao.,Chen Chao.,Fu YongNan.,Shen Qiang.,...&Zhang YouYi.(2011).Distinct actions of intermittent and sustained beta-adrenoceptor stimulation on cardiac remodeling.SCIENCE CHINA-LIFE SCIENCES,54(6),493-501.
MLA Ma XiaoWei,et al."Distinct actions of intermittent and sustained beta-adrenoceptor stimulation on cardiac remodeling".SCIENCE CHINA-LIFE SCIENCES 54.6(2011):493-501.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Ma XiaoWei]的文章
[Song Yao]的文章
[Chen Chao]的文章
百度学术
百度学术中相似的文章
[Ma XiaoWei]的文章
[Song Yao]的文章
[Chen Chao]的文章
必应学术
必应学术中相似的文章
[Ma XiaoWei]的文章
[Song Yao]的文章
[Chen Chao]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。