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学科主题临床医学
SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation
Zhuang, Xin-Jie1; Shi, Yu-Qiang2; Xu, Bo3; Chen, Lei4; Tang, Wen-Hao5; Huang, Jin1; Lian, Ying1; Liu, Ping1; Qiao, Jie1
关键词SLX2 BLOS2 Spindle Meiosis Oocyte
刊名CELL CYCLE
2014-07-15
DOI10.4161/cc.29265
13期:14页:2230-2236
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
资助者National Natural Science Foundation of China for Young Scholars ; National Natural Science Foundation of China ; National Natural Science Foundation of China for Young Scholars ; National Natural Science Foundation of China
研究领域[WOS]Cell Biology
关键词[WOS]GAMMA-TUBULIN COMPLEX ; MEIOSIS ; PROTEINS ; CELLS ; GENE ; PROLIFERATION ; RECOMBINATION ; PROGRESSION ; SYNAPSIS ; PROPHASE
英文摘要

Gametogenesis is a complex biological process of producing cells for sexual reproduction. Xlr super family members containing a conserved COR1 domain play essential roles in gametogenesis. In the present study, we identified that Slx2, a novel member of Xlr super family, is specifically expressed in the meiotic oocytes, which is demonstrated by western blotting and immunohistochemistry studies. In the first meiotic prophase, SLX2 is unevenly distributed in the nuclei of oocytes, during which phase SLX2 is partly co-localized with SYCP3 in synaptonemal complex and gamma H2AX in the nucleus of oocytes. Interestingly, the localization of SLX2 was found to be switched into the cytoplasm of oocytes after prometaphase I during oocyte maturation. Furthermore, yeast two-hybrid and coimmunoprecipitation studies demonstrated that SLX2 interacts with BLOS2, which is a novel centrosome-associated protein, and co-localized with.-Tubulin, which is a protein marker of chromosome segregation in meiosis. These results indicated that SLX2 might get involved in chromosomes segregation during meiosis by interaction with BLOS2. In conclusion, SLX2 might be a novel gametogenesis-related protein that could play multiple roles in regulation of meiotic processes including synaptonemal complex assembly and chromosome segregation.

语种英语
所属项目编号81200466 ; 81070534
资助者National Natural Science Foundation of China for Young Scholars ; National Natural Science Foundation of China ; National Natural Science Foundation of China for Young Scholars ; National Natural Science Foundation of China
WOS记录号WOS:000340272100014
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61857
Collection北京大学第三临床医学院_泌尿外科
作者单位1.Peking Univ, Dept Urol, Hosp 3, Beijing 100871, Peoples R China
2.Weifang Univ, Coll Bioengn, Weifang, Peoples R China
3.Peking Univ, Key Lab Assisted Reprod, Dept Obstet & Gynecol, Ctr Reprod Med,Minist Educ,Hosp 3, Beijing 100871, Peoples R China
4.Anhui Med Univ, Anhui Prov Hosp, Ctr Reprod Med, Hefei, Peoples R China
5.Zhengzhou Univ, Affiliated Hosp 1, Reprod Med Ctr, Zhengzhou 450052, Peoples R China
Recommended Citation
GB/T 7714
Zhuang, Xin-Jie,Shi, Yu-Qiang,Xu, Bo,et al. SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation[J]. CELL CYCLE,2014,13(14):2230-2236.
APA Zhuang, Xin-Jie.,Shi, Yu-Qiang.,Xu, Bo.,Chen, Lei.,Tang, Wen-Hao.,...&Qiao, Jie.(2014).SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation.CELL CYCLE,13(14),2230-2236.
MLA Zhuang, Xin-Jie,et al."SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation".CELL CYCLE 13.14(2014):2230-2236.
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