Long-term, efficient inhibition of microRNRNA function in mice using rAAV vectors

doi:10.1038/NMETH.1903

学科主题	基础医学
	Long-term, efficient inhibition of microRNRNA function in mice using rAAV vectors
	Xie, Jun 1,2; Ameres, Stefan L.3,4; Friedline, Randall 5,6; Hung, Jui-Hung 7,8; Zhang, Yu 1,9; Xie, Qing1,10 ; Zhong, Li 1,11; Su, Qin 1; He, Ran 1; Li, Mengxin 1,2; Li, Huapeng 1,2; Mu, Xin 1,12; Zhang, Hongwei 1,2; Broderick, Jennifer A.3; Kim, Jason K.5,6; Weng, Zhiping 7; Flotte, Terence R.1,2,13; Zamore, Phillip D.3,14; Gao, Guangping 1,2
刊名	NATURE METHODS
	2012-04-01
DOI	10.1038/NMETH.1903
卷	9 期:4 页:403-U123
收录类别	SCI
文章类型	Article
WOS标题词	Science & Technology
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	IN-VIVO ; ADENOASSOCIATED VIRUSES ; NONHUMAN-PRIMATES ; MAMMALIAN-CELLS ; MIR-122 ; TARGETS ; BIOLOGY ; MICRORNA-122 ; SPECIFICITY ; SUPPRESSION
英文摘要	Understanding the function of individual microRNA (miRNA) species in mice would require the production of hundreds of loss-of-function strains. To accelerate analysis of miRNA biology in mammals, we combined recombinant adeno-associated virus (rAAV) vectors with miRNA ′tough decoys′ (TuDs) to inhibit specific miRNAs. Intravenous injection of rAAV9 expressing anti-miR-122 or anti-let-7 TuDs depleted the corresponding miRNA and increased its mRNA targets. rAAV producing anti-miR-122 TuD but not anti-let-7 TuD reduced serum cholesterol by >30% for 25 weeks in wild-type mice. High-throughput sequencing of liver miRNAs from the treated mice confirmed that the targeted miRNAs were depleted and revealed that TuDs induced miRNA tailing and trimming in vivo. rAAV-mediated miRNA inhibition thus provides a simple way to study miRNA function in adult mammals and a potential therapy for dyslipidemia and other diseases caused by miRNA deregulation.
语种	英语
WOS记录号	WOS:000302218500026
项目编号	ALTF 522-2008 ; J2832-B09 ; GM62862 ; GM65236 ; UL1RR031982 ; U24-DK093000 ; P01 DK58327 ; P30 DK32520
资助机构	European Molecular Biology Organization ; Erwin Schrodinger-Auslandsstipendium ; US National Institutes of Health ; University of Massachusetts Medical School ; US National Institute of Diabetes and Digestive and Kidney Diseases
引用统计	被引频次：83[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.bjmu.edu.cn/handle/400002259/61874
专题	北京大学基础医学院_病原生物学系北京大学临床肿瘤学院_核医学科
作者单位	1.Boston Univ, Bioinformat Program, Boston, MA 02215 USA 2.Univ Massachusetts, Sch Med, Gene Therapy Ctr, Worcester, MA 01605 USA 3.Univ Massachusetts, Sch Med, Dept Microbiol & Physiol Syst, Worcester, MA USA 4.Univ Massachusetts, Sch Med, Dept Biochem & Mol Pharmacol, Worcester, MA USA 5.Austrian Acad Sci, Inst Mol Biotechnol, A-1010 Vienna, Austria 6.Univ Massachusetts, Sch Med, Mouse Phenotyping Ctr, Worcester, MA USA 7.Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA USA 8.Univ Massachusetts, Sch Med, Program Bioinformat & Integrat Biol, Worcester, MA USA 9.Sichuan Univ, W China Sch Clin Med, Ctr Canc, Dept Thorac Canc,W China Hosp, Chengdu 610064, Peoples R China 10.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China 11.Univ Massachusetts, Sch Med, Dept Med, Worcester, MA USA 12.Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Dermatol, Xian, Peoples R China 13.Univ Massachusetts, Sch Med, Dept Pediat, Worcester, MA USA 14.Univ Massachusetts, Sch Med, Howard Hughes Med Inst, Worcester, MA 01605 USA
推荐引用方式 GB/T 7714	Xie, Jun,Ameres, Stefan L.,Friedline, Randall,et al. Long-term, efficient inhibition of microRNRNA function in mice using rAAV vectors[J]. NATURE METHODS,2012,9(4):403-U123.
APA	Xie, Jun.,Ameres, Stefan L..,Friedline, Randall.,Hung, Jui-Hung.,Zhang, Yu.,...&Gao, Guangping.(2012).Long-term, efficient inhibition of microRNRNA function in mice using rAAV vectors.NATURE METHODS,9(4),403-U123.
MLA	Xie, Jun,et al."Long-term, efficient inhibition of microRNRNA function in mice using rAAV vectors".NATURE METHODS 9.4(2012):403-U123.

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