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学科主题基础医学
Long-term, efficient inhibition of microRNRNA function in mice using rAAV vectors
Xie, Jun1,2; Ameres, Stefan L.3,4; Friedline, Randall5,6; Hung, Jui-Hung7,8; Zhang, Yu1,9; Xie, Qing1,10; Zhong, Li1,11; Su, Qin1; He, Ran1; Li, Mengxin1,2; Li, Huapeng1,2; Mu, Xin1,12; Zhang, Hongwei1,2; Broderick, Jennifer A.3; Kim, Jason K.5,6; Weng, Zhiping7; Flotte, Terence R.1,2,13; Zamore, Phillip D.3,14; Gao, Guangping1,2
刊名NATURE METHODS
2012-04-01
DOI10.1038/NMETH.1903
9期:4页:403-U123
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]IN-VIVO ; ADENOASSOCIATED VIRUSES ; NONHUMAN-PRIMATES ; MAMMALIAN-CELLS ; MIR-122 ; TARGETS ; BIOLOGY ; MICRORNA-122 ; SPECIFICITY ; SUPPRESSION
英文摘要

Understanding the function of individual microRNA (miRNA) species in mice would require the production of hundreds of loss-of-function strains. To accelerate analysis of miRNA biology in mammals, we combined recombinant adeno-associated virus (rAAV) vectors with miRNA ′tough decoys′ (TuDs) to inhibit specific miRNAs. Intravenous injection of rAAV9 expressing anti-miR-122 or anti-let-7 TuDs depleted the corresponding miRNA and increased its mRNA targets. rAAV producing anti-miR-122 TuD but not anti-let-7 TuD reduced serum cholesterol by >30% for 25 weeks in wild-type mice. High-throughput sequencing of liver miRNAs from the treated mice confirmed that the targeted miRNAs were depleted and revealed that TuDs induced miRNA tailing and trimming in vivo. rAAV-mediated miRNA inhibition thus provides a simple way to study miRNA function in adult mammals and a potential therapy for dyslipidemia and other diseases caused by miRNA deregulation.

语种英语
WOS记录号WOS:000302218500026
项目编号ALTF 522-2008 ; J2832-B09 ; GM62862 ; GM65236 ; UL1RR031982 ; U24-DK093000 ; P01 DK58327 ; P30 DK32520
资助机构European Molecular Biology Organization ; Erwin Schrodinger-Auslandsstipendium ; US National Institutes of Health ; University of Massachusetts Medical School ; US National Institute of Diabetes and Digestive and Kidney Diseases
引用统计
被引频次:91[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61874
专题北京大学基础医学院_病原生物学系
北京大学临床肿瘤学院_核医学科
作者单位1.Boston Univ, Bioinformat Program, Boston, MA 02215 USA
2.Univ Massachusetts, Sch Med, Gene Therapy Ctr, Worcester, MA 01605 USA
3.Univ Massachusetts, Sch Med, Dept Microbiol & Physiol Syst, Worcester, MA USA
4.Univ Massachusetts, Sch Med, Dept Biochem & Mol Pharmacol, Worcester, MA USA
5.Austrian Acad Sci, Inst Mol Biotechnol, A-1010 Vienna, Austria
6.Univ Massachusetts, Sch Med, Mouse Phenotyping Ctr, Worcester, MA USA
7.Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA USA
8.Univ Massachusetts, Sch Med, Program Bioinformat & Integrat Biol, Worcester, MA USA
9.Sichuan Univ, W China Sch Clin Med, Ctr Canc, Dept Thorac Canc,W China Hosp, Chengdu 610064, Peoples R China
10.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
11.Univ Massachusetts, Sch Med, Dept Med, Worcester, MA USA
12.Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Dermatol, Xian, Peoples R China
13.Univ Massachusetts, Sch Med, Dept Pediat, Worcester, MA USA
14.Univ Massachusetts, Sch Med, Howard Hughes Med Inst, Worcester, MA 01605 USA
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GB/T 7714
Xie, Jun,Ameres, Stefan L.,Friedline, Randall,et al. Long-term, efficient inhibition of microRNRNA function in mice using rAAV vectors[J]. NATURE METHODS,2012,9(4):403-U123.
APA Xie, Jun.,Ameres, Stefan L..,Friedline, Randall.,Hung, Jui-Hung.,Zhang, Yu.,...&Gao, Guangping.(2012).Long-term, efficient inhibition of microRNRNA function in mice using rAAV vectors.NATURE METHODS,9(4),403-U123.
MLA Xie, Jun,et al."Long-term, efficient inhibition of microRNRNA function in mice using rAAV vectors".NATURE METHODS 9.4(2012):403-U123.
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