|Detention of HPV L1 Capsid Protein and hTERC Gene in Screening of Cervical Cancer|
|Huang Bin; Wu Ruifang; Li Ruizhen; Liang Yiheng; Liu Zhihong; Li Juan; Wang Chun; Zhou Yanqiu; Weng Leiming|
|关键词||Cervical lesion Human papilloma virus Human telomerase RNA component L1 capsid protein Squamous cell carcinomas|
|刊名||IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, Research & Experimental ; Pharmacology & Pharmacy|
|研究领域[WOS]||Research & Experimental Medicine ; Pharmacology & Pharmacy|
|关键词[WOS]||SQUAMOUS INTRAEPITHELIAL LESIONS ; HUMAN-PAPILLOMAVIRUS INFECTION ; TELOMERASE ACTIVITY ; NATURAL-HISTORY ; FREQUENT GAIN ; COPY NUMBER ; TERC ; EXPRESSION ; 3Q26 ; AMPLIFICATION|
Objective(s): To investigate the expression of human papilloma virus (HPV) L1 capsid protein, and human telomerase RNA component (hTERC) in cervical cancer and the role of detection of both genes in screening of cervical cancer.
Materials and Methods: A total of 309 patients were recruited and cervical exfoliated cells were collected. Immunocytochemistry was employed to detect HPV L1 capsid protein, and fluorescent in situ hybridization (FISH) was performed to detect the hTERC.
Results: The expression of HPV L1 capsid protein reduced with the increase of the histological grade of cervical cells and was negatively related to the grade of cervical lesions. However, the expression of hTERC increased with the increase of the histological grade and positively associated with the grade of cervical lesions. The proportion of patients with L1(-)/hTERC(+) was higher in patients with histological grade of CIN2 or higher than that in those with histological grade of CIN1. The L1(+)/hTERC(-) and L1(-)/hTERC(-) were negatively related to the grade of cervical lesions. L1(-)/hTERC(+) was positively associated with the grade of cervical lesions. The L1/hTERC ratio increased. The negative predictive value of both HPV L1 and hTERC was higher than that of HPV L1 or hTERC, but there was no marked difference in the screening efficacy of cervical cancer among HPV L1, hTERC and HPV L1+hTERC.
Conclusion: HPV L1 capsid protein and hTERC gene may serve as markers for the early diagnosis and prediction of cervical lesions. The increase in L1/hTERC ratio reflects the progression of cervical lesions to a certain extent.
|资助机构||Shenzhen Science and Technology Bureau|
|作者单位||Beijing Univ, Dept Gynecol & Obstet, Shenzhen Hosp, Shenzhen 518036, Peoples R China|
|Huang Bin,Wu Ruifang,Li Ruizhen,et al. Detention of HPV L1 Capsid Protein and hTERC Gene in Screening of Cervical Cancer[J]. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES,2013,16(6):797-802.|
|APA||Huang Bin.,Wu Ruifang.,Li Ruizhen.,Liang Yiheng.,Liu Zhihong.,...&Weng Leiming.(2013).Detention of HPV L1 Capsid Protein and hTERC Gene in Screening of Cervical Cancer.IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES,16(6),797-802.|
|MLA||Huang Bin,et al."Detention of HPV L1 Capsid Protein and hTERC Gene in Screening of Cervical Cancer".IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES 16.6(2013):797-802.|