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学科主题: 基础医学
题名:
Angiotensin-Converting Enzyme 2 Attenuates Bleomycin-Induced Lung Fibrosis in Mice
作者: Wang, Lifang1,2,3; Wang, Yuxiang3,4; Yang, Tuo1,2; Guo, Yanfei1,2; Sun, Tieying1,2
关键词: Lung fibrosis ; Alveolitis ; Bleomycin ; RAS ; ACE2
刊名: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
发表日期: 2015-05-01
DOI: 10.1159/000430131
卷: 36, 期:2, 页:697-711
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Physiology
研究领域[WOS]: Cell Biology ; Physiology
关键词[WOS]: IDIOPATHIC PULMONARY-FIBROSIS ; ALVEOLAR EPITHELIAL-CELLS ; SURFACTANT PROTEIN-A ; EXPRESSION ; APOPTOSIS ; CYTOKINES ; DISEASE ; MODEL ; BETA ; PATHOGENESIS
英文摘要:

Background: Local renin-angiotensin system (RAS) activation has been shown to play an important role in the pathogenesis of idiopathic pulmonary fibrosis (IPF). It has been reported that angiotensin-converting enzyme 2 (ACE2) could inhibit RAS-mediated epithelial injury and fibrogenesis and that ACE2 deficiency could aggravate acute and chronic lung injury. Through research, it could be deduced that ACE2 could protect against pulmonary fibrosis as a therapeutic target. Methods: Time-course analysis of the pathological characteristics of bleomycin-induced lung fibrosis was undertaken in a mouse model, and the effect of exogenous ACE2 on lung fibrosis was studied. Immunohistchemistry (IHC) staining and western blot (WB) testing for AGT and ACE2 were performed to evaluate the regulation of local RAS. TUNEL staining was used to observe epithelial apoptosis. Leukocyte common antigen (LCA) and pulmonary surfactant-associated protein A (SP-A) IHC staining and WB testing were performed to assess the inflammatory response and epithelial regeneration. Masson′s staining and a hydroxyproline assay were performed to examine collagen deposition. IHC staining and WB testing for TGF-beta 1 and alpha-SMA were performed to investigate the regulation of pro-fibrotic cytokines and the activation of fibroblasts. Results: Exogenous ACE2 attenuated bleomycin-induced lung fibrosis by reversing the reduction of local ACE2 and by suppressing the elevation of AGT. ACE2 decreased the apoptosis index and LCA levels and ameliorated the dynamic change in SP-A level, thus protecting against epithelial injury. Reductions of TGF-beta 1 and a-SMA were also found in ACE2-treated mice, indicating the inhibition of fibrogenesis. Conclusion: ACE2 attenuated bleomycin-induced lung fibrosis as an anti-inflammatory, antiapoptotic and anti-fibrotic agent, and it might be a promising therapeutic target for IPF. Copyright (C) 2015 S. Karger AG, Basel

语种: 英语
WOS记录号: WOS:000355944000024
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61896
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.Peking Univ, Sch Clin Med 5, Beijing 100871, Peoples R China
2.Peking Univ, Sch Clin Med 3, Beijing 100871, Peoples R China
3.Beijing Hosp, Dept Resp & Crit Care Med, Minist Hlth, Beijing 100730, Peoples R China
4.Peking Univ, Dept Pathol, Hlth Sci Ctr, Beijing 100871, Peoples R China

Recommended Citation:
Wang, Lifang,Wang, Yuxiang,Yang, Tuo,et al. Angiotensin-Converting Enzyme 2 Attenuates Bleomycin-Induced Lung Fibrosis in Mice[J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2015,36(2):697-711.
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