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SEPARATION AND PURIFICATION OF TRITERPENE SAPONINS FROM ROOTS OF RADIX PHYTOLACCAE BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY COUPLED WITH EVAPORATIVE LIGHT SCATTERING DETECTION
Ma, Jie2; Chen, Qianliang2; Lai, Daowan3; Sun, Wenji2; Zhang, Tianyou4; Ito, Yoichiro1
关键词high-speed countercurrent chromatography Radix phytolaccae triterpene saponins
刊名JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
2010
DOI10.1080/10826070903574659
33期:4页:563-571
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PREPARATIVE ISOLATION ; ESCULENTA
英文摘要

Coupled with evaporative light scattering detection, high-speed countercurrent chromatography was successfully applied for the first time for separation and purification of four triterpene saponins including esculentosides A, B, C, and D from the roots of Radix phytolaccae. The separation was performed with an optimized two-phase solvent system composed of chloroform-methanol-water (4:4:2,v/v), using the lower phase as the mobile phase at a flow rate of 1.5mL/min. From 150mg of crude extract, 46.3mg of esculentoside A, 21.8mg of esculentoside B, 7.3mg of esculentoside C, and 13.6mg of esculentoside D were obtained at purities of 96.7%, 99.2%, 96.5%, and 97.8%, respectively, as determined by HPLC analysis. The structures of the four triterpene saponins were identified by ESI-MS,1H NMR and 13C NMR.

语种英语
WOS记录号WOS:000274536100013
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61899
专题北京大学药学院
作者单位1.Beijing Inst New Technol Applicat, Beijing, Peoples R China
2.NHLBI, NIH, Bethesda, MD 20892 USA
3.Northwest Univ, Biomed Key Lab Shaanxi Prov, Xian, Peoples R China
4.Peking Univ, Sch Pharm, Hlth Sci Ctr, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Ma, Jie,Chen, Qianliang,Lai, Daowan,et al. SEPARATION AND PURIFICATION OF TRITERPENE SAPONINS FROM ROOTS OF RADIX PHYTOLACCAE BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY COUPLED WITH EVAPORATIVE LIGHT SCATTERING DETECTION[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(4):563-571.
APA Ma, Jie,Chen, Qianliang,Lai, Daowan,Sun, Wenji,Zhang, Tianyou,&Ito, Yoichiro.(2010).SEPARATION AND PURIFICATION OF TRITERPENE SAPONINS FROM ROOTS OF RADIX PHYTOLACCAE BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY COUPLED WITH EVAPORATIVE LIGHT SCATTERING DETECTION.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,33(4),563-571.
MLA Ma, Jie,et al."SEPARATION AND PURIFICATION OF TRITERPENE SAPONINS FROM ROOTS OF RADIX PHYTOLACCAE BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY COUPLED WITH EVAPORATIVE LIGHT SCATTERING DETECTION".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 33.4(2010):563-571.
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