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学科主题: 基础医学
题名:
Comparison of nucleic acid-based detection of avian influenza H5N1 with virus isolation
作者: Shan, SH; Ko, LS; Collins, RA; Wu, ZL; Chen, JH; Chan, KY; Xing, J; Lau, LT; Yu, ACH
关键词: highly pathogenic avian influenza ; nucleic acid sequence-based amplification ; H5N1 electrochemiluminescence ; virus isolation
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2003-03-07
DOI: 10.1016/S0006-291X(03)00165-7
卷: 302, 期:2, 页:377-383
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: A VIRUSES ; HONG-KONG ; PCR ASSAY ; SAMPLES ; NASBA
英文摘要:

Nucleic acid sequence-based amplification with electrochemiluminescent detection (NASBA/ECL) of avian influenza virus was compared with viral culture in embryonated chicken eggs. Virus was isolated from blood or anal swabs of chickens artificially infected with highly pathogenic avian influenza A/Chicken/Hong Kong/1000/97 (H5N1). Viral nucleic acid was detected in blood samples by NASBA/ECL immediately prior to death, whilst nucleic acid extracted from anal swabs was detected from the day following artificial infection until death. Thus, blood and/or anal swabs are a suitable source of material for the detection of avian influenza in dead birds, but anal swabs are more suitable for detection of viral genetic material in live birds. Dilution of a known viral standard was used to determine the limit of sensitivity for both NASBA/ECL and egg Culture detection methods. The NASBA/ ECL method was equivalent in sensitivity to egg culture. The NASBA/ECL results agreed with egg culture data in 71/94 (75.5%) tissue samples obtained from artificially infected birds. (C) 2003 Elsevier Science (USA). All rights reserved.

语种: 英语
WOS记录号: WOS:000181534500031
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61908
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Hong Kong DNA Chips Ltd, Kowloon, Hong Kong, Peoples R China
2.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
3.Peking Univ, Dept Neurobiol, Beijing 100083, Peoples R China
4.Shanghai Entry Exit Inspect & Quarantine Bur, Shanghai 200135, Peoples R China

Recommended Citation:
Shan, SH,Ko, LS,Collins, RA,et al. Comparison of nucleic acid-based detection of avian influenza H5N1 with virus isolation[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2003,302(2):377-383.
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