IR@PKUHSC  > 北京大学基础医学院  > 神经生物学系
学科主题基础医学
Comparison of nucleic acid-based detection of avian influenza H5N1 with virus isolation
Shan, SH; Ko, LS; Collins, RA; Wu, ZL; Chen, JH; Chan, KY; Xing, J; Lau, LT; Yu, ACH
关键词highly pathogenic avian influenza nucleic acid sequence-based amplification H5N1 electrochemiluminescence virus isolation
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2003-03-07
DOI10.1016/S0006-291X(03)00165-7
302期:2页:377-383
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]A VIRUSES ; HONG-KONG ; PCR ASSAY ; SAMPLES ; NASBA
英文摘要

Nucleic acid sequence-based amplification with electrochemiluminescent detection (NASBA/ECL) of avian influenza virus was compared with viral culture in embryonated chicken eggs. Virus was isolated from blood or anal swabs of chickens artificially infected with highly pathogenic avian influenza A/Chicken/Hong Kong/1000/97 (H5N1). Viral nucleic acid was detected in blood samples by NASBA/ECL immediately prior to death, whilst nucleic acid extracted from anal swabs was detected from the day following artificial infection until death. Thus, blood and/or anal swabs are a suitable source of material for the detection of avian influenza in dead birds, but anal swabs are more suitable for detection of viral genetic material in live birds. Dilution of a known viral standard was used to determine the limit of sensitivity for both NASBA/ECL and egg Culture detection methods. The NASBA/ ECL method was equivalent in sensitivity to egg culture. The NASBA/ECL results agreed with egg culture data in 71/94 (75.5%) tissue samples obtained from artificially infected birds. (C) 2003 Elsevier Science (USA). All rights reserved.

语种英语
WOS记录号WOS:000181534500031
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61908
专题北京大学基础医学院_神经生物学系
作者单位1.Hong Kong DNA Chips Ltd, Kowloon, Hong Kong, Peoples R China
2.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
3.Peking Univ, Dept Neurobiol, Beijing 100083, Peoples R China
4.Shanghai Entry Exit Inspect & Quarantine Bur, Shanghai 200135, Peoples R China
推荐引用方式
GB/T 7714
Shan, SH,Ko, LS,Collins, RA,et al. Comparison of nucleic acid-based detection of avian influenza H5N1 with virus isolation[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2003,302(2):377-383.
APA Shan, SH.,Ko, LS.,Collins, RA.,Wu, ZL.,Chen, JH.,...&Yu, ACH.(2003).Comparison of nucleic acid-based detection of avian influenza H5N1 with virus isolation.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,302(2),377-383.
MLA Shan, SH,et al."Comparison of nucleic acid-based detection of avian influenza H5N1 with virus isolation".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 302.2(2003):377-383.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Comparison of nuclei(103KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Shan, SH]的文章
[Ko, LS]的文章
[Collins, RA]的文章
百度学术
百度学术中相似的文章
[Shan, SH]的文章
[Ko, LS]的文章
[Collins, RA]的文章
必应学术
必应学术中相似的文章
[Shan, SH]的文章
[Ko, LS]的文章
[Collins, RA]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Comparison of nucleic acid-based detection of avian influenza H5N1 with virus isolation.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。