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学科主题: 临床医学
题名:
Hydrogen Sulfide Inhibits L-Type Calcium Currents Depending upon the Protein Sulfhydryl State in Rat Cardiomyocytes
作者: Zhang, Rongyuan1; Sun, Yan1; Tsai, Haojan2; Tang, Chaoshu3,4; Jin, Hongfang1; Du, Junbao1,4
刊名: PLOS ONE
发表日期: 2012-05-10
DOI: 10.1371/journal.pone.0037073
卷: 7, 期:5
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: VENTRICULAR MYOCYTES ; CHANNELS ; SUBUNIT ; HEART ; CA2+ ; DEPOLARIZATION ; MECHANISMS ; RESIDUES ; SYSTEM ; PORE
英文摘要:

Hydrogen sulfide (H2S) is a novel gasotransmitter that inhibits L-type calcium currents (I (Ca, L)). However, the underlying molecular mechanisms are unclear. In particular, the targeting site in the L-type calcium channel where H2S functions remains unknown. The study was designed to investigate if the sulfhydryl group could be the possible targeting site in the L-type calcium channel in rat cardiomyocytes. Cardiac function was measured in isolated perfused rat hearts. The L-type calcium currents were recorded by using a whole cell voltage clamp technique on the isolated cardiomyocytes. The L-type calcium channel containing free sulfhydryl groups in H9C2 cells were measured by using Western blot. The results showed that sodium hydrosulfide (NaHS, an H2S donor) produced a negative inotropic effect on cardiac function, which could be partly inhibited by the oxidant sulfhydryl modifier diamide (DM). H2S donor inhibited the peak amplitude of I (Ca, L) in a concentration-dependent manner. However, dithiothreitol (DTT), a reducing sulfhydryl modifier markedly reversed the H2S donor-induced inhibition of I (Ca, L) in cardiomyocytes. In contrast, in the presence of DM, H2S donor could not alter cardiac function and L-type calcium currents. After the isolated rat heart or the cardiomyocytes were treated with DTT, NaHS could markedly alter cardiac function and L-type calcium currents in cardiomyocytes. Furthermore, NaHS could decrease the functional free sulfhydryl group in the L-type Ca2+ channel, which could be reversed by thiol reductant, either DTT or reduced glutathione. Therefore, our results suggest that H2S might inhibit L-type calcium currents depending on the sulfhydryl group in rat cardiomyocytes.

语种: 英语
所属项目编号: 2011CB503904 ; 2012CB517806 ; 30821001 ; 30801251 ; 7112130
项目资助者: Major Basic Research Development Program of People&prime ; s Republic of China ; National Natural Science Foundation of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000305336400055
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61921
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Minist Educ, Key Lab Mol Cardiovasc Dis, Beijing, Peoples R China
2.Peking Univ, Hosp 1, Dept Pediat, Beijing 100871, Peoples R China
3.Peking Univ, Hosp 1, Dept Cent Lab, Beijing 100871, Peoples R China
4.Peking Univ, Hosp 1, Inst Cardiovasc Res, Beijing 100871, Peoples R China

Recommended Citation:
Zhang, Rongyuan,Sun, Yan,Tsai, Haojan,et al. Hydrogen Sulfide Inhibits L-Type Calcium Currents Depending upon the Protein Sulfhydryl State in Rat Cardiomyocytes[J]. PLOS ONE,2012,7(5).
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