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学科主题临床医学
Intestinal Alkaline Phosphatase Inhibits the Translocation of Bacteria of Gut-Origin in Mice with Peritonitis: Mechanism of Action
Wang, Wei1; Chen, Shan-Wen1; Zhu, Jing1; Zuo, Shuai1; Ma, Yuan-Yuan2; Chen, Zi-Yi1; Zhang, Jun-Ling1; Chen, Guo-Wei1; Liu, Yu-Cun1; Wang, Peng-Yuan1
刊名PLOS ONE
2015-05-06
DOI10.1371/journal.pone.0124835
10期:5
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]ENDOTHELIAL GROWTH-FACTOR ; CLAUDIN-2 EXPRESSION ; VEGF EXPRESSION ; INFLAMMATION ; MICROBIOTA ; COLITIS ; DISEASE ; LIPOPOLYSACCHARIDE ; PERMEABILITY ; INDUCTION
英文摘要

Exogenous intestinal alkaline phosphatase (IAP), an enzyme produced endogenously at the brush edge of the intestinal mucosa, may mitigate the increase in aberrant intestinal permeability increased during sepsis. The aim of this study was to test the efficacy of the inhibitory effect of IAP on acute intestinal inflammation and to study the molecular mechanisms underlying IAP in ameliorating intestinal permeability. We used an in vivo imaging method to evaluate disease status and the curative effect of IAP. Two Escherichia coli (E. coli) B21 strains, carrying EGFP labeled enhanced green fluorescent protein (EGFP) and RFP labeled red fluorescent protein (RFP), were constructed as tracer bacteria and were administered orally to C57/B6N mice to generate an injection peritonitis (IP) model. The IP model was established by injecting inflammatory lavage fluid. C57/B6N mice bearing the tracer bacteria were subsequently treated with (IP+IAP group), or without IAP (IP group). IAP was administered to the mice via tail vein injections. The amount of tracer bacteria in the blood, liver, and lungs at 24 h post-injection was analyzed via flow cytometry (FCM), in vivo imaging, and Western blotting. Intestinal barrier function was measured using a flux assay with the macro-molecule fluorescein isothiocyanate dextran, molecular weight 40kD, (FD40). To elucidate the molecular mechanism underlying the effects of IAP, we examined the levels of ERK phosphorylation, and the expression levels of proteins in the ERK-SP1-VEGF and ERK-Cdx-2-Claudin-2 pathways. We observed that IAP inhibited the expression of Claudin-2, a type of cation channel-forming protein, and VEGF, a cytokine that may increase intestinal permeability by reducing the levels of dephosphorylated ERK. In conclusion, exogenous IAP shows a therapeutic effect in an injection peritonitis model. This including inhibition of bacterial translocation. Moreover, we have established an imaging methodology for live-animals can effectively evaluate intestinal permeability and aberrant bacterial translocation in IP models.

语种英语
WOS记录号WOS:000354049700051
项目编号201196005
资助机构China Health and Medical Development Foundation (Grant: Research on Surgical Infection Mechanism and Control) ; Research Fund of Peking University First Hospital
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61927
专题北京大学第一临床医学院_普通外科
作者单位1.Peking Univ, Hosp 1, Dept Surg, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 1, Ctr Expt Anim, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Wang, Wei,Chen, Shan-Wen,Zhu, Jing,et al. Intestinal Alkaline Phosphatase Inhibits the Translocation of Bacteria of Gut-Origin in Mice with Peritonitis: Mechanism of Action[J]. PLOS ONE,2015,10(5).
APA Wang, Wei.,Chen, Shan-Wen.,Zhu, Jing.,Zuo, Shuai.,Ma, Yuan-Yuan.,...&Wang, Peng-Yuan.(2015).Intestinal Alkaline Phosphatase Inhibits the Translocation of Bacteria of Gut-Origin in Mice with Peritonitis: Mechanism of Action.PLOS ONE,10(5).
MLA Wang, Wei,et al."Intestinal Alkaline Phosphatase Inhibits the Translocation of Bacteria of Gut-Origin in Mice with Peritonitis: Mechanism of Action".PLOS ONE 10.5(2015).
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