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学科主题: 基础医学
题名:
Identification of transcripts and translatants targeted by overexpressed PCBP1
作者: Huo, Li-Rong2; Zhong, Nanbert1,2
关键词: PCBP1 ; Overexpression ; Transcriptional profile ; Translational profile ; Proteomic study
刊名: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
发表日期: 2008-11-01
DOI: 10.1016/j.bbapap.2008.06.017
卷: 1784, 期:11, 页:1524-1533
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: NUCLEAR RIBONUCLEOPROTEIN E1 ; MESSENGER-RNA ; GENE-EXPRESSION ; 3&prime ; -UNTRANSLATED REGION ; DOMAIN PROTEINS ; HNRNP E1 ; BINDING ; CANCER ; CELLS ; COMPLEXES
英文摘要:

PCBP1 is a member of the hnRNP family that functions as a RNA-binding, as well as DNA-binding, protein. The detailed transcripts and translatants targeted by PCBP1 at a global level are not yet known. We undertook an investigation of transcriptional and translational profiles after overexpressing exogenous PCBP1 in SH-SY5Y cells. Our results in two independent studies showed that 601 transcripts, including 26 down-regulated transcripts and 575 up-regulated transcripts, were impacted by overexpression of exogenous PCBP1. However, 138 and 144 transcripts showed non-overlapped differential expression in each study. These altered transcripts are clustered mainly in metabolic and transcriptional regulations. Proteomic profiles detected with a two-dimensional chromatographic PF2D showed a global change of translations, mainly in a range of pI = 4.96-5.76 and pI = 7.96-8.36. Three predominant proteins, which were differentially less expressed in PCBP1 overexpression cells and were detected at pI = 7.96-8.16, were identified as histone proteins, indicating that histone proteins are among the targets regulated by PCBP1. Our investigation has opened a new avenue for further studying the biological functions of PCBP1. (C) 2008 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 985-2-035-39 ; 2007CB511902 ; 30671157
项目资助者: Chinese Ministry of Education ; Ministry of Science and Technology ; National Nature Science Foundation ; New York State Office of Mental Retardation and Developmental Disabilities (NYS OMRDD)
WOS记录号: WOS:000261019000006
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61929
Appears in Collections:基础医学院_北京大学医学遗传中心_期刊论文

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作者单位: 1.New York State Inst Basic Res Dev Disabil, Dept Human Genet, Staten Isl, NY 10314 USA
2.Peking Univ, Ctr Med Genet, Beijing 100871, Peoples R China

Recommended Citation:
Huo, Li-Rong,Zhong, Nanbert. Identification of transcripts and translatants targeted by overexpressed PCBP1[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2008,1784(11):1524-1533.
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