学科主题基础医学
Identification of transcripts and translatants targeted by overexpressed PCBP1
Huo, Li-Rong2; Zhong, Nanbert1,2
关键词PCBP1 Overexpression Transcriptional profile Translational profile Proteomic study
刊名BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
2008-11-01
DOI10.1016/j.bbapap.2008.06.017
1784期:11页:1524-1533
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]NUCLEAR RIBONUCLEOPROTEIN E1 ; MESSENGER-RNA ; GENE-EXPRESSION ; 3&prime ; -UNTRANSLATED REGION ; DOMAIN PROTEINS ; HNRNP E1 ; BINDING ; CANCER ; CELLS ; COMPLEXES
英文摘要

PCBP1 is a member of the hnRNP family that functions as a RNA-binding, as well as DNA-binding, protein. The detailed transcripts and translatants targeted by PCBP1 at a global level are not yet known. We undertook an investigation of transcriptional and translational profiles after overexpressing exogenous PCBP1 in SH-SY5Y cells. Our results in two independent studies showed that 601 transcripts, including 26 down-regulated transcripts and 575 up-regulated transcripts, were impacted by overexpression of exogenous PCBP1. However, 138 and 144 transcripts showed non-overlapped differential expression in each study. These altered transcripts are clustered mainly in metabolic and transcriptional regulations. Proteomic profiles detected with a two-dimensional chromatographic PF2D showed a global change of translations, mainly in a range of pI = 4.96-5.76 and pI = 7.96-8.36. Three predominant proteins, which were differentially less expressed in PCBP1 overexpression cells and were detected at pI = 7.96-8.16, were identified as histone proteins, indicating that histone proteins are among the targets regulated by PCBP1. Our investigation has opened a new avenue for further studying the biological functions of PCBP1. (C) 2008 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000261019000006
项目编号985-2-035-39 ; 2007CB511902 ; 30671157
资助机构Chinese Ministry of Education ; Ministry of Science and Technology ; National Nature Science Foundation ; New York State Office of Mental Retardation and Developmental Disabilities (NYS OMRDD)
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61929
专题北京大学基础医学院_北京大学医学遗传中心
作者单位1.New York State Inst Basic Res Dev Disabil, Dept Human Genet, Staten Isl, NY 10314 USA
2.Peking Univ, Ctr Med Genet, Beijing 100871, Peoples R China
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Huo, Li-Rong,Zhong, Nanbert. Identification of transcripts and translatants targeted by overexpressed PCBP1[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2008,1784(11):1524-1533.
APA Huo, Li-Rong,&Zhong, Nanbert.(2008).Identification of transcripts and translatants targeted by overexpressed PCBP1.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,1784(11),1524-1533.
MLA Huo, Li-Rong,et al."Identification of transcripts and translatants targeted by overexpressed PCBP1".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1784.11(2008):1524-1533.
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