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学科主题临床医学
Sensitive Quantification of Bulleyaconitine A in Human Serum by Liquid Chromatography-Tandem Mass Spectrometry
Xiong, Xin1,2; Zhai, Suodi1,2; Yao, Zhongqiang3
关键词bulleyaconitine A liquid chromatography-tandem mass spectrometry human serum
刊名BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
2009-07-01
DOI10.1271/bbb.90064
73期:7页:1572-1577
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry, Applied ; Food Science & Technology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology
关键词[WOS]ACONITUM ALKALOIDS ; URINE SAMPLES ; PLANTS ; BLOOD
英文摘要

A simple and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated for the quantification of bulleyaconitine A (BLA) in human serum. Samples were extracted from human serum by protein precipitation. Domperidone was used as internal standard (I.S.). Chromatography separation was performed on a Waters Sunfire C18 column (2.1 x 50 mm, 5 mu m) with a gradient mobile phase. Detection was performed in positive mode using multiple reaction monitoring (MRM) of the transition m/z 644.2 > 584.3 for BLA and m/z 426.3 > 175.3 for I.S. The proposed method was validated in a linear range of 0.0587-11.7 ng/ml. Intra- and inter-day precision was better than 9.88% and 7.76%. The recovery for BLA was 97.90%, and for I.S. 89.40%. This method provides a simple, rapid, specific, and sensitive tool for the quantitativedetermination of BLA in human serum, and can be used to monitor serum levels in patients treated with BLA.

语种英语
WOS记录号WOS:000268691600019
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61956
专题北京大学第三临床医学院_药剂科
作者单位1.Peking Univ, Hosp 3, Dept Pharm, Beijing 100191, Peoples R China
2.Peking Univ, Therapeut Drug Monitoring & Clin Toxicol Ctr, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Dept Rheumatol & Immunol, Beijing 100191, Peoples R China
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GB/T 7714
Xiong, Xin,Zhai, Suodi,Yao, Zhongqiang. Sensitive Quantification of Bulleyaconitine A in Human Serum by Liquid Chromatography-Tandem Mass Spectrometry[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2009,73(7):1572-1577.
APA Xiong, Xin,Zhai, Suodi,&Yao, Zhongqiang.(2009).Sensitive Quantification of Bulleyaconitine A in Human Serum by Liquid Chromatography-Tandem Mass Spectrometry.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,73(7),1572-1577.
MLA Xiong, Xin,et al."Sensitive Quantification of Bulleyaconitine A in Human Serum by Liquid Chromatography-Tandem Mass Spectrometry".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 73.7(2009):1572-1577.
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