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学科主题: 基础医学
题名:
Effect of 2-amino-2-[2-(4-octylphenyl) ethyl] propane-1,3-diol hydrochloride (FTY 720) on immune liver injury in mice
作者: He, Jing-Hua; Zhang, Hui-Na; Lin, Zhi-Bin
关键词: Immune liver injury ; FTY 720 ; Apoptosis ; Cell proliferation
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2005-01-28
卷: 11, 期:4, 页:573-576
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
英文摘要:

AIM: To investigate the protective effect against two immune liver injury models in mice by 2-amino-2-[ 2-(4-octylphenyl) ethyl] propane-1,3-diol hydrochloride and its possible mechanisms in Con A-induced liver damage.

METHODS: Liver tissue or hepatocyte injury was monitored biochemically by measuring alanine aminotransferase (sALT) and aspartate aminotransferase (sAST) activity. Hematoxylin & eosin (HE) staining was used for histopathological examination. To evaluate the role of IFN-gamma and IL-4 in the liver injury, serum levels of IFN-gamma and IL-4 were determined using commercially available ELISA kit at 12 h after Con A challenge. We also determined FTY 720-induced spleen cell apoptosis by flow cytometry analysis or spleen cell proliferation test.

RESULTS: Different doses of FTY 720 treatment dramatically reduced circulating markers of hepatocyte injury in two kinds of immunological liver injury models. FTY 720 dramatically reduced the elevated serum IFN-gamma and IL-4 levels after Con A injection. Effect of spleen cell supernatants treated with Con A or FTY 720 on hepatocytes showed that ALT activities in cultured hepatocyte supernatants in Con A treatment group increased markedly and FTY 720 could reduce this elevated ALT activities in FTY 720 treatment group. FTY 720 dose-dependently increased the percentage of apoptotic cells in T cells and inhibited splenocyte proliferation induced by Con A.

CONCLUSION: Pretreatment with FTY 720 was shown to produce protective effect on the immune liver injury in mice. The possible mechanism of FTY 720 on Con A-induced liver damage is that it could inhibit lymphocyte proliferation and induce lymphocyte apoptosis, resulting in the reduction of IL-4 or IFN-gamma release, and subsequently protecting liver from being damaged by Con A. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000208099700022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61966
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Basic Med Sch, Beijing 100083, Peoples R China

Recommended Citation:
He, Jing-Hua,Zhang, Hui-Na,Lin, Zhi-Bin. Effect of 2-amino-2-[2-(4-octylphenyl) ethyl] propane-1,3-diol hydrochloride (FTY 720) on immune liver injury in mice[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2005,11(4):573-576.
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