IR@PKUHSC  > 基础医学院  > 北京大学干细胞研究中心
学科主题基础医学
Derivation of embryonic stem cell line from frozen human embryos and neural differentiation
Tan, Ji-chun1,2; Li, Yang3; Qu, Wen-yu2; Liu, Li-ying2; Jiang, Li2; Sun, Kai-lai1
关键词embryonic stem cells frozen human embryo neural differentiation
刊名NEUROREPORT
2008-10-08
DOI10.1097/WNR.0b013e32830e4c35
19期:15页:1451-1455
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]IN-VITRO DIFFERENTIATION ; HUMAN BLASTOCYSTS ; CYCLIC-AMP ; PROGENITORS ; NEURONS ; EXPRESS
英文摘要

We established a human embryonic stem cell line derived from frozen human embryos of Chinese origin. The cell line expressed the pluripotent markers SSEA-4,TRA-1-60,TRA-1-81, Oct-4, and alkaline phosphatase. The pluripotency of the cell line was also demonstrated in vivo by teratoma formation in severe combined immunodeficiency mice. The embryonic stem cells formed embryoid bodies after culturing in suspension for 7 days. The embryoid bodies were transferred to an adherent culture system in serum-free medium. The differentiating cells derived from the embryoid bodies expressed Nestin and Sox2, markers of neural progenitor cells. After the induction of cyclic AMP for 7 days, the neural progenitor cells had differentiated into neurons and glial cells.

语种英语
WOS记录号WOS:000259728400002
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61974
专题基础医学院_北京大学干细胞研究中心
作者单位1.China Med Univ, Dept Med Genet, Shenyang 110012, Peoples R China
2.Shenyang Gynecol & Obstet Hosp, Reprod Ctr, Shenyang, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Stem Cell Res Ctr, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Tan, Ji-chun,Li, Yang,Qu, Wen-yu,et al. Derivation of embryonic stem cell line from frozen human embryos and neural differentiation[J]. NEUROREPORT,2008,19(15):1451-1455.
APA Tan, Ji-chun,Li, Yang,Qu, Wen-yu,Liu, Li-ying,Jiang, Li,&Sun, Kai-lai.(2008).Derivation of embryonic stem cell line from frozen human embryos and neural differentiation.NEUROREPORT,19(15),1451-1455.
MLA Tan, Ji-chun,et al."Derivation of embryonic stem cell line from frozen human embryos and neural differentiation".NEUROREPORT 19.15(2008):1451-1455.
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