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学科主题临床医学
One-step Articular Cartilage Repair: Combination of In Situ Bone Marrow Stem Cells With Cell-free Poly(L-lactic-co-glycolic Acid) Scaffold in a Rabbit Model
Shi, Junjun1; Zhang, Xin1; Zeng, Xiangzhu2; Zhu, Jingxian1; Pi, Yanbin1; Zhou, Chunyan3; Ao, Yingfang1
刊名ORTHOPEDICS
2012-05-01
DOI10.3928/01477447-20120426-20
35期:5页:E665-E671
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Orthopedics
研究领域[WOS]Orthopedics
关键词[WOS]AUTOLOGOUS CHONDROCYTE IMPLANTATION ; MICROFRACTURE TECHNIQUE ; DIFFERENTIATION ; KNEE ; LESIONS ; GROWTH ; NICHE ; TRANSPLANTATION ; DEFECTS ; MATRIX
英文摘要

Focal full-thickness articular cartilage defects are challenging to repair. The purpose of this study was to find a simple, effective 1-step articular cartilage repair method. Because stem cell niches produce a microenvironment for stem cell self-renewal, proliferation, and differentiation, we integrated in situ bone marrow stem cells with an implanted poly(L-lactic-co-glycolic acid) (PLLGA) scaffold. Marrow stem cells grew and proliferated on cell-free PLLGA scaffolds, which were evaluated by scanning electronic microscopy (SEM) and Cell Counting Kit-8 (Dojindo, Kumamoto, Japan). Twenty-seven rabbits (54 knees) with large cylinder femoral trochlear cartilage defects were created and repaired with microfracture and cell-free PLLGA scaffold implantation (group 1), microfracture (group 2), or cell-free PLLGA scaffold implantation (group 3).

Outcomes were evaluated by magnetic resonance imaging, International Cartilage Repair Society scores, histology, and immunohistochemistry. The repair effects were better in group 1 than in groups 2 and 3. In group 1, hyaline-like cartilage formed at week 24. Magnetic resonance imaging showed homogeneous signals as the adjacent normal cartilage. Collagen type II and toluidine blue were stained positively as normal cartilage tissue, and the color and thickness of regenerated tissue were similar to surrounding normal tissue. The combination of microfracture and cell-free PLLGA scaffold implantation used endogenous marrow stem cells in situ and promoted hyaline-like cartilage regeneration rapidly and effectively.

语种英语
WOS记录号WOS:000304363100009
项目编号81071474 ; BMU2009129-112 ; 20100001110086
资助机构National Natural Science Foundation of China ; Program for Changjiang Scholars and Innovative Research Team in University ; Research Fund for the Doctoral Program of Higher Education of China
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61982
专题北京大学第三临床医学院_运动医学研究所
北京大学基础医学院
北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 3, Dept Diagnost Radiol, Beijing 100871, Peoples R China
3.Peking Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Hlth Sci Ctr, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Shi, Junjun,Zhang, Xin,Zeng, Xiangzhu,et al. One-step Articular Cartilage Repair: Combination of In Situ Bone Marrow Stem Cells With Cell-free Poly(L-lactic-co-glycolic Acid) Scaffold in a Rabbit Model[J]. ORTHOPEDICS,2012,35(5):E665-E671.
APA Shi, Junjun.,Zhang, Xin.,Zeng, Xiangzhu.,Zhu, Jingxian.,Pi, Yanbin.,...&Ao, Yingfang.(2012).One-step Articular Cartilage Repair: Combination of In Situ Bone Marrow Stem Cells With Cell-free Poly(L-lactic-co-glycolic Acid) Scaffold in a Rabbit Model.ORTHOPEDICS,35(5),E665-E671.
MLA Shi, Junjun,et al."One-step Articular Cartilage Repair: Combination of In Situ Bone Marrow Stem Cells With Cell-free Poly(L-lactic-co-glycolic Acid) Scaffold in a Rabbit Model".ORTHOPEDICS 35.5(2012):E665-E671.
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