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学科主题: 临床医学
题名:
Reduction of major histocompatibility complex class I expression on bladder carcinoma following tumor antigen-pulsed dendritic cell vaccine: Implications for immunoresistance in therapy
作者: Li, Mengqiang1; Wang, Huili2; Xi, Zhijun3; Xu, Enci1; Jiang, Xuejun4; Chen, Jiangying1; Shi, Wenzhen1
关键词: bladder tumor ; dendritic cells ; major histocompatibility complex class I molecule ; rat ; vaccine
刊名: INTERNATIONAL JOURNAL OF UROLOGY
发表日期: 2010-07-01
DOI: 10.1111/j.1442-2042.2010.02540.x
卷: 17, 期:7, 页:666-672
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: METHYL-N-NITROSOUREA ; PROTECTIVE IMMUNITY ; T-LYMPHOCYTES ; CANCER ; MHC ; IMMUNOTHERAPY ; ACTIVATION ; GENERATION ; MELANOMA ; EFFICACY
英文摘要:

Objectives:

To clarify the relationship between a decreased major histocompatibility complex class I (MHC-I) expression on bladder tumors and decreased immunological efficacy of tumor antigen-pulsed dendritic cell vaccine in a rat bladder carcinoma model induced by N-methyl-N-nitrosourea irrigation.

Methods:

Enzyme-linked immunosorbent assay was used to evaluate interferon-gamma concentration in the serum and colorimetric lactate dehydrogenase release assay in vitro was used to test the cytotoxicity capability of T lymphocytes. MHC-I expression on tumor cells was detected by flow cytometry and analyzed with CellQuest software.

Results:

The tumor antigen sensitized dendritic cell vaccine group showed decreased hyperplastic formations, lower pathological stages in rat bladders and more potent cytotoxicity activity (P < 0.001) than the dendritic cell vaccine group. Additionally, immunization with pulsed dendritic cell vaccine induced higher specific cytokine production of interferon-gamma. Nevertheless, a decreased MHC-I expression on bladder tumors was tested after immunotherapy by pulsed dendritic cell vaccine on week 15. As expected, the cytotoxic activity of T lymphocytes from rats on tumor cells with low MHC-I expression was also decreased to 19.70 +/- 4.82% as compared with tumor cells with high MHC-I (52.10 +/- 8.66%, P = 0.005).

Conclusions:

Tumor antigen sensitized dendritic cell vaccine has beneficial activity on N-methyl-N-nitrosourea-induced bladder cancer in situ in rats, but therapeutic responses are accompanied by decreased MHC-I expression on tumors, possibly suggesting poor long-term therapeutic outcomes.

语种: 英语
所属项目编号: 2009J05069 ; 2009J05067 ; JA09113
项目资助者: Fujian Natural Sciences Foundation ; Fujian Education Department Foundation
WOS记录号: WOS:000279022600020
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61984
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Fujian Med Univ, Dept Immunol, Fuzhou, Peoples R China
2.Peking Univ, Hosp 1, Dept Urol, Beijing 100871, Peoples R China
3.Fujian Med Univ, Affiliated Union Hosp, Dept Urol, Fuzhou, Peoples R China
4.Chinese Acad Sci, Inst Microbiol, Ctr Mol Immunol, Beijing, Peoples R China

Recommended Citation:
Li, Mengqiang,Wang, Huili,Xi, Zhijun,et al. Reduction of major histocompatibility complex class I expression on bladder carcinoma following tumor antigen-pulsed dendritic cell vaccine: Implications for immunoresistance in therapy[J]. INTERNATIONAL JOURNAL OF UROLOGY,2010,17(7):666-672.
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