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Inhibition of androgen induces autophagy in benign prostate epithelial cells
Li, Mengqiang1; Yang, Xu1; Wang, Huili2; Xu, Enci1; Xi, Zhijun3
关键词5-alpha reductase inhibitor autophagy benign prostatic hyperplasia dihydrotestosterone
刊名INTERNATIONAL JOURNAL OF UROLOGY
2014-02-01
DOI10.1111/iju.12210
21期:2页:195-199
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]IMMUNOHISTOCHEMICAL ANALYSIS ; NATURAL-HISTORY ; HYPERPLASIA ; FINASTERIDE ; CANCER ; DIHYDROTESTOSTERONE ; DUTASTERIDE ; SUPPRESSION ; EXPRESSION ; APOPTOSIS
英文摘要

Objective: 5-alpha Reductase inhibitor can reduce the volume of benign prostatic hyperplasia by lowering benign prostatic hyperplasia level and consequently inducing epithelial cells apoptosis. The present study investigated whether autophagy and apoptosis of benign prostatic hyperplasia epithelial cells are influenced by low benign prostatic hyperplasia levels.

Methods: PWR-1E prostate epithelial cells transfected with GFP-LC3 plasmid were subjected to androgen deprivation conditions. Then the autophagic puncta were evaluated by fluorescence microscopy, and the cellular apoptosis rate was detected by 4, 6-diamidino-2-phenylindole staining after blocking of autophagic process by 3-methyladenine. Furthermore, autophagy status was also determined in hyperplasia prostate tissues from 5-alpha reductase inhibitor-treated patients by immunohistochemistry.

Results: In the androgen deprivation medium, autophagic punta increased markedly in PWR-1E cells, and blockage of autophagy by 3-methyladenine significantly promoted PWR-1E cells′ apoptosis rate. In vivo, the expression of LC3 protein (an important autophagic marker) in hyperplasia prostate tissue significantly increased after 5-alpha reductase inhibitor treatment. Meanwhile, the prostate-specific antigen, as an inner control, decreased.

Conclusion: 5-alpha Reductase inhibitor treatment increases autophagy and possibly decreases the apoptosis of prostate epithelial cells.

语种英语
WOS记录号WOS:000342573800023
资助机构College Teacher fund
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61995
专题北京大学第一临床医学院_泌尿外科
作者单位1.Fujian Med Univ, Union Hosp, Dept Urol, Fuzhou 350001, Peoples R China
2.Fujian Med Univ, Dept Immunol, Fuzhou 350001, Peoples R China
3.Peking Univ, Hosp 1, Dept Urol, Beijing 100871, Peoples R China
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Li, Mengqiang,Yang, Xu,Wang, Huili,et al. Inhibition of androgen induces autophagy in benign prostate epithelial cells[J]. INTERNATIONAL JOURNAL OF UROLOGY,2014,21(2):195-199.
APA Li, Mengqiang,Yang, Xu,Wang, Huili,Xu, Enci,&Xi, Zhijun.(2014).Inhibition of androgen induces autophagy in benign prostate epithelial cells.INTERNATIONAL JOURNAL OF UROLOGY,21(2),195-199.
MLA Li, Mengqiang,et al."Inhibition of androgen induces autophagy in benign prostate epithelial cells".INTERNATIONAL JOURNAL OF UROLOGY 21.2(2014):195-199.
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