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学科主题: 临床医学
题名:
Inhibition of androgen induces autophagy in benign prostate epithelial cells
作者: Li, Mengqiang1; Yang, Xu1; Wang, Huili2; Xu, Enci1; Xi, Zhijun3
关键词: 5-alpha reductase inhibitor ; autophagy ; benign prostatic hyperplasia ; dihydrotestosterone
刊名: INTERNATIONAL JOURNAL OF UROLOGY
发表日期: 2014-02-01
DOI: 10.1111/iju.12210
卷: 21, 期:2, 页:195-199
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: IMMUNOHISTOCHEMICAL ANALYSIS ; NATURAL-HISTORY ; HYPERPLASIA ; FINASTERIDE ; CANCER ; DIHYDROTESTOSTERONE ; DUTASTERIDE ; SUPPRESSION ; EXPRESSION ; APOPTOSIS
英文摘要:

Objective: 5-alpha Reductase inhibitor can reduce the volume of benign prostatic hyperplasia by lowering benign prostatic hyperplasia level and consequently inducing epithelial cells apoptosis. The present study investigated whether autophagy and apoptosis of benign prostatic hyperplasia epithelial cells are influenced by low benign prostatic hyperplasia levels.

Methods: PWR-1E prostate epithelial cells transfected with GFP-LC3 plasmid were subjected to androgen deprivation conditions. Then the autophagic puncta were evaluated by fluorescence microscopy, and the cellular apoptosis rate was detected by 4, 6-diamidino-2-phenylindole staining after blocking of autophagic process by 3-methyladenine. Furthermore, autophagy status was also determined in hyperplasia prostate tissues from 5-alpha reductase inhibitor-treated patients by immunohistochemistry.

Results: In the androgen deprivation medium, autophagic punta increased markedly in PWR-1E cells, and blockage of autophagy by 3-methyladenine significantly promoted PWR-1E cells′ apoptosis rate. In vivo, the expression of LC3 protein (an important autophagic marker) in hyperplasia prostate tissue significantly increased after 5-alpha reductase inhibitor treatment. Meanwhile, the prostate-specific antigen, as an inner control, decreased.

Conclusion: 5-alpha Reductase inhibitor treatment increases autophagy and possibly decreases the apoptosis of prostate epithelial cells.

语种: 英语
项目资助者: College Teacher fund
WOS记录号: WOS:000342573800023
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61995
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Fujian Med Univ, Union Hosp, Dept Urol, Fuzhou 350001, Peoples R China
2.Fujian Med Univ, Dept Immunol, Fuzhou 350001, Peoples R China
3.Peking Univ, Hosp 1, Dept Urol, Beijing 100871, Peoples R China

Recommended Citation:
Li, Mengqiang,Yang, Xu,Wang, Huili,et al. Inhibition of androgen induces autophagy in benign prostate epithelial cells[J]. INTERNATIONAL JOURNAL OF UROLOGY,2014,21(2):195-199.
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