IR@PKUHSC  > 北京大学第三临床医学院  > 骨科
学科主题临床医学
Icariine stimulates proliferation and differentiation of human osteoblasts by increasing production of bone morphogenetic protein 2
Yin Yiao-xue; Chen Zhong-qiang; Liu Zhong-jun; Ma Qing-jun; Dang Geng-ting
关键词Icariine osteoblasts cell proliferation cell differentiation bone morphogenetic protein
刊名CHINESE MEDICAL JOURNAL
2007-02-05
120期:3页:204-210
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]CELLS
英文摘要

Background lcariine is a flavonoid isolated from a traditional Chinese medicine Epimedium pubescens and is the main active compound of it. Recently, Epimedium pubescens was found to have a therapeutic effect on osteoporosis. But the mechanism is unclear. The aim of the study was to research the effect of Icariine on the proliferation and differentiation of human osteoblasts.

Methods Human osteoblasts were obtained by inducing human marrow mesenchymal stem cells (hMSCs) directionally and were cultured in the presence of various concentrations of Icariine. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test was used to observe the effect of lcariine on cell proliferation. The activity of alkaline phosphatase (ALP) and the amount of calcified nodules were assayed to observe the effect on cell differentiation. The expression of bone morphogenetic protein 2 (BMP-2) mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR).

Results Icariine (20 mu g/ml) increased significantly the proliferation of human osteoblasts. And, Icariine (10 mu g/ml and 20 mu g/ml) increased the activity of ALP and the amount of calcified nodules of human osteoblasts significantly (P < 0.05). BMP-2 mRNA synthesis was elevated significantly in response to Icariine (20 mu g/ml).

Conclusions Icariine has a direct stimulatory effect on the proliferation and differentiation of cultured human osteoblast cells in vitro, which may be mediated by increasing production of BMP-2 in osteoblasts.

语种英语
WOS记录号WOS:000244222200006
引用统计
被引频次:39[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62011
专题北京大学第三临床医学院_骨科
作者单位Peking Univ Third Hosp, Dept Orthopaed, Beijing 100083, Peoples R China
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GB/T 7714
Yin Yiao-xue,Chen Zhong-qiang,Liu Zhong-jun,et al. Icariine stimulates proliferation and differentiation of human osteoblasts by increasing production of bone morphogenetic protein 2[J]. CHINESE MEDICAL JOURNAL,2007,120(3):204-210.
APA Yin Yiao-xue,Chen Zhong-qiang,Liu Zhong-jun,Ma Qing-jun,&Dang Geng-ting.(2007).Icariine stimulates proliferation and differentiation of human osteoblasts by increasing production of bone morphogenetic protein 2.CHINESE MEDICAL JOURNAL,120(3),204-210.
MLA Yin Yiao-xue,et al."Icariine stimulates proliferation and differentiation of human osteoblasts by increasing production of bone morphogenetic protein 2".CHINESE MEDICAL JOURNAL 120.3(2007):204-210.
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