|Biological Fractionation of Lead Isotopes in Sprague-Dawley Rats Lead Poisoned via the Respiratory Tract|
|Wu, Jing1,3; Liu, Duojian1; Xie, Qing1,2; Wang, Jingyu1,2|
|WOS标题词||Science & Technology|
|研究领域[WOS]||Science & Technology - Other Topics|
|关键词[WOS]||EXPOSURE ; CHILDREN ; THRESHOLD ; TOXICITY ; RATIOS ; BLOOD ; REDUCTION ; POLLUTION ; CADMIUM ; ZINC|
Objectives: It was considered that lead isotope ratios did not change during physical, chemical, or biological processes. Thus, lead isotope ratios have been used as fingerprints to identify possible lead sources. However, recent evidence has shown that the lead isotope ratios among different biological samples in human are not always identical from its lead origins in vitro. An animal experiment was conducted to explore the biological fractionation of lead isotopes in biological systems.
Methods: 24 male Sprague-Dawley (SD) rats were divided into groups that received acute lead exposure (0, 0.02, 0.2, or 2 mg/kg body weight of lead acetate) via the respiratory route every day for 5 days. Biological samples (i.e., blood, urine, and feces) were collected for comparison with the lead acetate (test substance) and the low-lead animal feed (diet) administered to the rats. The lead isotope ratios were determined by inductively coupled plasma mass spectrometry (ICP-MS).
Results: There are significant differences (p<0.05) in lead isotope ratios between blood, urine, and feces. Moreover, a nonlinear relationship between the blood lead concentration and the blood lead isotope ratios was observed. There is also a threshold effect to the fractionation function. Only the blood isotope ratio of Pb-204/Pb-206 matches the test substance well. As for feces, when Pb-204/Pb-206 ratio is considered, there is no significant difference between feces-test substance pairs in medium and high dose group.
Conclusions: The biological fractionation of lead isotopes in SD rats was observed. Moreover, there might be a threshold for the biological fractionation of lead isotopes which is depending on whole blood lead level. It is considered to be more reliable that we compared the isotope ratios of potential lead hazards with both blood and feces lead fingerprints especially for Pb-204/Pb-206 ratio under high-dose exposure.
|资助机构||Natural Science Foundation of China|
|作者单位||1.Peking Univ, Sch Publ Hlth, Beijing 100871, Peoples R China|
2.Peking Univ, Ctr Med & Hlth Anal, Beijing 100871, Peoples R China
3.Hebei Univ Technol, Sch Chem Engn & Technol, Tianjin, Peoples R China
|Wu, Jing,Liu, Duojian,Xie, Qing,et al. Biological Fractionation of Lead Isotopes in Sprague-Dawley Rats Lead Poisoned via the Respiratory Tract[J]. PLOS ONE,2012,7(12).|
|APA||Wu, Jing,Liu, Duojian,Xie, Qing,&Wang, Jingyu.(2012).Biological Fractionation of Lead Isotopes in Sprague-Dawley Rats Lead Poisoned via the Respiratory Tract.PLOS ONE,7(12).|
|MLA||Wu, Jing,et al."Biological Fractionation of Lead Isotopes in Sprague-Dawley Rats Lead Poisoned via the Respiratory Tract".PLOS ONE 7.12(2012).|