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学科主题: 临床医学
题名:
Comparison of characteristics of natural autoantibodies against myeloperoxidase and anti-myeloperoxidase autoantibodies from patients with microscopic polyangiitis
作者: Xu, Peng-Cheng1; Cui, Zhao1; Chen, Min1; Hellmark, Thomas2; Zhao, Ming-Hui1
关键词: Natural autoantibodies ; Myeloperoxidase ; Anti-neutrophil cytoplasm antibodies ; Microscopic polyangitis ; Vasculitis
刊名: RHEUMATOLOGY
发表日期: 2011-07-01
DOI: 10.1093/rheumatology/ker085
卷: 50, 期:7, 页:1236-1243
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Rheumatology
研究领域[WOS]: Rheumatology
关键词[WOS]: ANTINEUTROPHIL CYTOPLASMIC ANTIBODIES ; IGG SUBCLASS DISTRIBUTION ; GLOMERULAR-BASEMENT-MEMBRANE ; SYSTEMIC VASCULITIS ; FOLLOW-UP ; DISEASE-ACTIVITY ; MPO-ANCA ; SERA ; PROTEINASE-3 ; NEUTROPHILS
英文摘要:

Methods. MPO-NAAs were affinity purified from normal plasma of five healthy blood donors and one batch of IVIG. MPO-ANCAs were purified from plasma of 10 patients with MPA. Antigen specificity of the antibodies was tested by western blot analysis. The titre, the avidity, the Immunoglobulin G (IgG) subclasses and the effect of the antibodies towards the binding between ceruloplasmin and MPO were tested using ELISAs. The MPO-NAA-induced production of reactive oxygen species was assessed using oxidation of dihydrorhodamine (DHR) to rhodamine.

Results. MPO-NAA recognized epitope(s) in the heavy chains of MPO with conformation-dependent structure, the same as MPO-ANCA. The median titre of MPO-NAA was lower than that of MPO-ANCA (1 : 40 vs 1 : 4800, P < 0.001). The median avidity of MPO-NAA was lower than that of MPO-ANCA (2.2 x 10(7) vs 8.7 x 10(7)/M, P = 0.014). The IgG subclasses of MPO-NAA were mainly restricted to IgG1 (100%) and lack of IgG3. The inhibition effect on the binding between ceruloplasmin and MPO was lower for MPO-NAA than MPO-ANCA (P = 0.046). The MPO-NAA-induced respiratory burst of neutrophils was significantly weaker than that of MPO-ANCA (P = 0.036).

Conclusion. The lower titre, lower avidity and lack of IgG3 subclass compared with MPO-ANCA may contribute to the non-pathogenic co-existence of MPO-NAA with MPO in serum.

语种: 英语
所属项目编号: 30700752 ; 30725034 ; 7082094
项目资助者: National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation
WOS记录号: WOS:000291746100011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62029
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Lund Univ, Dept Nephrol, S-22101 Lund, Sweden
2.Peking Univ, Hosp 1, Dept Med,Renal Div, Inst Nephrol,Key Lab Renal Dis,Minist Hlth China, Beijing 100034, Peoples R China

Recommended Citation:
Xu, Peng-Cheng,Cui, Zhao,Chen, Min,et al. Comparison of characteristics of natural autoantibodies against myeloperoxidase and anti-myeloperoxidase autoantibodies from patients with microscopic polyangiitis[J]. RHEUMATOLOGY,2011,50(7):1236-1243.
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