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学科主题基础医学
Responses of astrocyte to simultaneous glutamate and arachidonic acid treatment
Xu, Zhen Yu1,2,3,4; Liu, Hua Dong1,2,3; Lau, Lok Ting1,2,3; Yingge, Zhang5; Zhao, Rui1,2,3; Tong, Geng Lei4; Chan, Pak Hoo6; Yu, Albert Cheung Hoi1,2,3
关键词Astrocyte Caspase-3 Bcl-2 Hsp70 Synergistic Effect Afm
刊名NEUROCHEMISTRY INTERNATIONAL
2009-07-01
DOI10.1016/j.neuint.2009.02.016
55期:1-3页:143-150
收录类别SCI ; ISTP
文章类型Proceedings Paper
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]MITOCHONDRIAL PERMEABILITY TRANSITION ; ATOMIC-FORCE MICROSCOPY ; TRAUMATIC BRAIN-INJURY ; IN-VITRO ISCHEMIA ; CELL-DEATH ; CORTICAL-NEURONS ; PRIMARY CULTURES ; ION HOMEOSTASIS ; APOPTOSIS ; NEURODEGENERATION
英文摘要

After cellular injury many endogenous toxins are released from injured cells and result in secondary injury. To elucidate mechanisms of such injury many of these toxins have been studied individually. However, the data obtained is only useful for reference and does not accurately represent the multifactorial situation under pathophysiological conditions. Primary astrocytic cultures were treated individually and simultaneously with two well-studied toxins, glutamate (Glu) and arachidonic acid (AA). Both are simultaneously released from neural cells during injury. Measurements of cellular protein content, intracellular water space, lactate dehydrogenase release, and malondialdehyde formation indicated that Glu and AA act through different mechanisms. Glu + AA applied together had a synergistic effect on the levels of Caspase-3 gene expression, and Bcl-2 and Hsp70 protein. Atomic force microscopy observed that Glu caused cell membrane roughness and nuclear swelling, while AA induced pores in the cell membrane and nuclear shrinkage. Glu + AA accelerated nuclear shrinkage and resulted in more serious cell damage. This study not only distinguishes the different responses of astrocytes to Glu and AA, but also provides a new view into the synergistic effect of these biochemicals; highlighting the need to be cautious in applying single factor experimental data to interpret complex physiological and pathological conditions in animals. Two or more factors may act not only on different targets but also on the same target synergistically. (C) 2009 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000266283000022
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62051
专题基础医学院_神经生物学系
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Peking Univ, Key Lab Neurosci, Minist Educ, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Sch Basic Med Sci, Beijing 100191, Peoples R China
4.Shanghai Jiao Tong Univ, Inst Life Sci & Technol, Shanghai 200240, Peoples R China
5.Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
6.Stanford Univ, Sch Med, Dept Neurosurg, Stanford, CA 94305 USA
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Xu, Zhen Yu,Liu, Hua Dong,Lau, Lok Ting,et al. Responses of astrocyte to simultaneous glutamate and arachidonic acid treatment[J]. NEUROCHEMISTRY INTERNATIONAL,2009,55(1-3):143-150.
APA Xu, Zhen Yu.,Liu, Hua Dong.,Lau, Lok Ting.,Yingge, Zhang.,Zhao, Rui.,...&Yu, Albert Cheung Hoi.(2009).Responses of astrocyte to simultaneous glutamate and arachidonic acid treatment.NEUROCHEMISTRY INTERNATIONAL,55(1-3),143-150.
MLA Xu, Zhen Yu,et al."Responses of astrocyte to simultaneous glutamate and arachidonic acid treatment".NEUROCHEMISTRY INTERNATIONAL 55.1-3(2009):143-150.
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