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学科主题: 临床医学
题名:
The role of Id-1 in chemosensitivity and epirubicin-induced apoptosis in bladder cancer cells
作者: Hu, Hao1,2; Han, Hui Ying2; Wang, Yu Liang1; Zhang, Xiao Peng1; Chua, Chee Wai2; Wong, Y. C.2; Wang, Xiao Feng1; Ling, Ming Tat2; Xu, Ke Xin1
关键词: Id-1 ; bladder cancer cells ; chemosensitivity ; epirubicin ; apoptosis
刊名: ONCOLOGY REPORTS
发表日期: 2009-04-01
DOI: 10.3892/or_00000323
卷: 21, 期:4, 页:1053-1059
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: PROSTATE-CANCER ; PROGNOSTIC-SIGNIFICANCE ; SIGNALING PATHWAY ; PHASE-II ; CARCINOMA ; ACTIVATION ; EXPRESSION ; INACTIVATION ; GEMCITABINE ; PROTEINS
英文摘要:

Recurrence and progression are the major problems in the treatment of bladder cancer. Increased expression of Id-1, a basic helix-loop-helix transcription factor, has recently been shown in several types of advanced cancer. Some studies have provided evidence to suggest that Id-1 can be considered a potential therapeutic target. The objective of this study was to investigate the role of Id-1 in the chemosensitivity of bladder cancer cells, and the effect of Id-1 on chemotherapeutic drug-induced apoptosis in bladder cancer cells. We compared the different sensitivity to epirubicin in RT 112 and MGH-U1 cell lines with different Id-1 expression. Then, we transfected different vectors into RT112 and MGH-U1 respectively, and generated the stable Id-l up-regulation and down-regulation transfectants. The results of cell viability assay showed up-regulation of Id-1 in RT112 leading to increased sensitivity in response to epirubicin, and down-regulation of Id-1 increased cellular sensitivity to epirubicin. Furthermore, the analysis of apoptosis related protein revealed that up-regulation of Id-1 suppressed epirubicin-induced apoptosis and down-regulation of Id-l leading to increased epirubicin-induced apoptosis. Wound closure assay showed up-regulation of Id-1 leading to improved migration abilities of bladder cancer cells under chemotherapy. Our results suggest that up-regulation of Id-1 in bladder cancer cells lead to increased cell viability in response to epirubicin by its improved anti-apoptotic role, and down-regulation of Id-1 increases cellular sensitivity to epirubicin by increased anticancer drug-induced apoptosis.

语种: 英语
WOS记录号: WOS:000264696000032
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62052
Appears in Collections:北京大学第二临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Urol, Hlth Sci Ctr, Beijing 100044, Peoples R China
2.Univ Hong Kong, Fac Med, Dept Anat, Canc Biol Grp, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Hu, Hao,Han, Hui Ying,Wang, Yu Liang,et al. The role of Id-1 in chemosensitivity and epirubicin-induced apoptosis in bladder cancer cells[J]. ONCOLOGY REPORTS,2009,21(4):1053-1059.
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