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Simultaneous determination of nitrendipine and hydrochlorothiazide in spontaneously hypertensive rat plasma using HPLC with on-line solid-phase extraction
Shang, Dewei1,2; Wang, Xipei2; Zhao, Xiutai3; Huang, Fengming2; Tian, Ganzhong1; Lu, Wei1,2; Zhou, Tianyan1,2
关键词Nitrendipine Hydrochlorothiazide On-line solid-phase extraction Spontaneously hypertensive rat Pharmacokinetics
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2011-11-15
DOI10.1016/j.jchromb.2011.09.025
879期:30页:3459-3464
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
资助者Dionex China Ltd. ; Ministry of Science and Technology of the People&prime ; s Republic of China (Peking University) ; Dionex China Ltd. ; Ministry of Science and Technology of the People&prime ; s Republic of China (Peking University)
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]ISOLATED SYSTOLIC HYPERTENSION ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; CALCIUM-CHANNEL BLOCKADE ; OLDER PATIENTS ; ELECTROCHEMICAL DETECTION ; ACTIVE TREATMENT ; QUANTIFICATION ; PLACEBO ; BLOOD
英文摘要

A HPLC method with on-line solid phase extraction (SPE) and DAD detection was developed for the simultaneous determination of nitrendipine and hydrochlorothiazide in spontaneously hypertensive rat (SHR) plasma. Plasma samples (100 mu L) were injected directly onto a CAPCELL MF C(8) SPE column. High-abundance proteins and most matrixes in plasma were removed by on-line SPE technology, while nitrendipine and hydrochlorothiazide trapped on the SPE column were effectively separated on a C(18) analytical column. The column temperature was maintained at 20 degrees C. The optimal detection wavelength was 237 nm for NTDP and 271 nm for HCTZ. The total analytical run time was 34 min. The proposed method was linear over the range 5-500 ngmL(-1) for nitrendipine and 10-1000 ng mL(-1) for hydrochlorothiazide. The lower limit of detection (LLOD) was 0.5 and 0.6 ng mL(-1) for nitrendipine and hydrochlorothiazide, respectively. The sensitivity and precision of the method were within acceptable limits during validation period. The method was successfully used to investigate the pharmacokinetic characteristics of nitrendipine and hydrochlorothiazide in spontaneously hypertensive rats. (C) 2011 Elsevier B.V. All rights reserved.

语种英语
所属项目编号2009ZX09301-010
资助者Dionex China Ltd. ; Ministry of Science and Technology of the People&prime ; s Republic of China (Peking University) ; Dionex China Ltd. ; Ministry of Science and Technology of the People&prime ; s Republic of China (Peking University)
WOS记录号WOS:000297396200015
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62055
Collection北京大学药学院
作者单位1.Dionex China Ltd, Beijing 100085, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Shang, Dewei,Wang, Xipei,Zhao, Xiutai,et al. Simultaneous determination of nitrendipine and hydrochlorothiazide in spontaneously hypertensive rat plasma using HPLC with on-line solid-phase extraction[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(30):3459-3464.
APA Shang, Dewei.,Wang, Xipei.,Zhao, Xiutai.,Huang, Fengming.,Tian, Ganzhong.,...&Zhou, Tianyan.(2011).Simultaneous determination of nitrendipine and hydrochlorothiazide in spontaneously hypertensive rat plasma using HPLC with on-line solid-phase extraction.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(30),3459-3464.
MLA Shang, Dewei,et al."Simultaneous determination of nitrendipine and hydrochlorothiazide in spontaneously hypertensive rat plasma using HPLC with on-line solid-phase extraction".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.30(2011):3459-3464.
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