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学科主题: 临床医学
题名:
Efficient growth suppression and apoptosis in human laryngeal carcinoma cell line HEP-2 induced by an adeno-associated virus expressing human FAS ligand
作者: Sun, Haili1; Liu, Yuhe1; Bu, Dingfang2; Liu, Xiang3; Norris, James S.3; Xiao, Shuifang1
关键词: adeno-associated virus ; human Fas ligand ; gene transfer ; apoptosis ; gene therapy
刊名: HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
发表日期: 2012-11-01
DOI: 10.1002/hed.21985
卷: 34, 期:11, 页:1628-1633
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Otorhinolaryngology ; Surgery
研究领域[WOS]: Otorhinolaryngology ; Surgery
关键词[WOS]: CANCER IN-VIVO ; GENE-THERAPY ; MELANOMA-CELLS ; UP-REGULATION ; NECK-CANCER ; HEAD ; CARCINOGENESIS ; PROGRESSION ; RESISTANCE ; ADENOVIRUS
英文摘要:

Background Apoptosis induced by Fas/FasL system has been proposed as a gene therapy methold for various cancers. Methods We used adeno-associated virusexpressing enhanced green fluorescent protein (EGFP)-human FasL (AAV-EGFP-hFasL) to deliver FasL into Hep-2 cells, cytotoxicity was detected by MTS assay , apoptosis was confirmed by flow cytometry. We also treated the xenograft of Hep-2 tumor in nude mice with intratumoral injection of AAV-EGFP-hFasL. The size of the xenograft, the apoptosis in the xenograft, and the survival rate of the inoculated mice were then evaluated. Results Hep-2 cells infected with AAV-EGFP-hFasL showed increased apoptosis rate and killing effect compared with AAV-EGFPinfected cells. In addition intratumoral injections of AAV-EGFP-hFasL into Hep-2 xenografts induced significant growth suppression of tumors. Conclusion Our findings suggest that the introduction of FasL into head and neck squamous cell carcinoma may induce significant apoptosis, and adeno-associated virus may be a useful vehicle for gene therapy. (C) 2012 Wiley Periodicals, Inc. Head Neck, 2012

语种: 英语
所属项目编号: 30672306
项目资助者: National Natural Science Fund of China
WOS记录号: WOS:000310337800018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62060
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Otolaryngol Head & Neck Surg, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 1, Lab Ctr, Beijing 100871, Peoples R China
3.Med Univ S Carolina, Dept Microbiol & Immunol, Charleston, SC 29425 USA

Recommended Citation:
Sun, Haili,Liu, Yuhe,Bu, Dingfang,et al. Efficient growth suppression and apoptosis in human laryngeal carcinoma cell line HEP-2 induced by an adeno-associated virus expressing human FAS ligand[J]. HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK,2012,34(11):1628-1633.
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