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Efficient growth suppression and apoptosis in human laryngeal carcinoma cell line HEP-2 induced by an adeno-associated virus expressing human FAS ligand
Sun, Haili1; Liu, Yuhe1; Bu, Dingfang2; Liu, Xiang3; Norris, James S.3; Xiao, Shuifang1
关键词adeno-associated virus human Fas ligand gene transfer apoptosis gene therapy
刊名HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
2012-11-01
DOI10.1002/hed.21985
34期:11页:1628-1633
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Otorhinolaryngology ; Surgery
研究领域[WOS]Otorhinolaryngology ; Surgery
关键词[WOS]CANCER IN-VIVO ; GENE-THERAPY ; MELANOMA-CELLS ; UP-REGULATION ; NECK-CANCER ; HEAD ; CARCINOGENESIS ; PROGRESSION ; RESISTANCE ; ADENOVIRUS
英文摘要

Background Apoptosis induced by Fas/FasL system has been proposed as a gene therapy methold for various cancers. Methods We used adeno-associated virusexpressing enhanced green fluorescent protein (EGFP)-human FasL (AAV-EGFP-hFasL) to deliver FasL into Hep-2 cells, cytotoxicity was detected by MTS assay , apoptosis was confirmed by flow cytometry. We also treated the xenograft of Hep-2 tumor in nude mice with intratumoral injection of AAV-EGFP-hFasL. The size of the xenograft, the apoptosis in the xenograft, and the survival rate of the inoculated mice were then evaluated. Results Hep-2 cells infected with AAV-EGFP-hFasL showed increased apoptosis rate and killing effect compared with AAV-EGFPinfected cells. In addition intratumoral injections of AAV-EGFP-hFasL into Hep-2 xenografts induced significant growth suppression of tumors. Conclusion Our findings suggest that the introduction of FasL into head and neck squamous cell carcinoma may induce significant apoptosis, and adeno-associated virus may be a useful vehicle for gene therapy. (C) 2012 Wiley Periodicals, Inc. Head Neck, 2012

语种英语
WOS记录号WOS:000310337800018
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62060
专题北京大学第一临床医学院
北京大学第一临床医学院_耳鼻咽喉头颈外科
作者单位1.Peking Univ, Hosp 1, Dept Otolaryngol Head & Neck Surg, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 1, Lab Ctr, Beijing 100871, Peoples R China
3.Med Univ S Carolina, Dept Microbiol & Immunol, Charleston, SC 29425 USA
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Sun, Haili,Liu, Yuhe,Bu, Dingfang,et al. Efficient growth suppression and apoptosis in human laryngeal carcinoma cell line HEP-2 induced by an adeno-associated virus expressing human FAS ligand[J]. HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK,2012,34(11):1628-1633.
APA Sun, Haili,Liu, Yuhe,Bu, Dingfang,Liu, Xiang,Norris, James S.,&Xiao, Shuifang.(2012).Efficient growth suppression and apoptosis in human laryngeal carcinoma cell line HEP-2 induced by an adeno-associated virus expressing human FAS ligand.HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK,34(11),1628-1633.
MLA Sun, Haili,et al."Efficient growth suppression and apoptosis in human laryngeal carcinoma cell line HEP-2 induced by an adeno-associated virus expressing human FAS ligand".HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK 34.11(2012):1628-1633.
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