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学科主题: 临床医学
题名:
cDNA microarray analysis and immunohistochemistry reveal a distinct molecular phenotype in serous endometrial cancer compared to endometrioid endometrial cancer
作者: Chen, Yonghua1; Yao, Yuanyang1; Zhang, Lili1; Li, Xiaoping1; Wang, Yue1; Zhao, Lijun1; Wang, Jianliu1; Wang, Gongwei2; Shen, Danhua2; Wei, Lihui1; Zhao, Jianqing3
关键词: Endometrioid adenocarcinoma ; Serous adenocarcinoma ; cDNA microarrays ; Immunohistochemistry
刊名: EXPERIMENTAL AND MOLECULAR PATHOLOGY
发表日期: 2011-08-01
DOI: 10.1016/j.yexmp.2011.04.005
卷: 91, 期:1, 页:373-384
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pathology
研究领域[WOS]: Pathology
关键词[WOS]: GENE-EXPRESSION PROFILES ; DIFFERENT HISTOLOGIC TYPES ; MICROSATELLITE INSTABILITY ; FUCOSYL-TRANSFERASE ; MESSENGER-RNA ; BREAST-CANCER ; ANNEXIN-IV ; CARCINOMA ; P53 ; OVEREXPRESSION
英文摘要:

Purpose: The main objective of this study was to refine more precisely the gene expression patterns used to distinguish serous from endometrioid endometrial carcinoma.

Methods: A low-density cDNA microarray containing 492 genes was designed and constructed. The gene expression profiles of 32 endometrioid and 5 serous endometrial cancer tissue samples were compared. The expression of 5 differentially expressed genes: NDC80, BUB1, FUT8, ANXA4 and BBC3 in endometrioid and serous adenocarcinoma samples was further evaluated by quantitative real-time PCR and immunohistochemistry.

Results: Unsupervised cluster analysis revealed that the 5 serous adenocarcinomas clustered together. These were separated from the endometrioid adenocarcinomas which were further sorted into 3 additional clusters. A comparative analysis indicated that there was a significant difference in FIGO stage with no significant difference in depth of myometrial invasion among the 4 clusters. The FIGO ternary grading system could not distinctly separate the 3 clusters of endometrioid adenocarcinomas, but a binary grading system was able to do so. Using a supervised analysis, we have identified 46 genes exhibiting >2-fold differences that can be used to statistically differentiate serous adenocarcinomas from endometrioid adenocarcinomas. The directions of gene and protein expression change of five differentially expressed genes estimated by real-time PCR and immunohistochemistry are consistent with those estimated from microarray.

Conclusions: Serous adenocarcinoma exhibits distinct gene expression profiles, compared with those of endometrioid adenocarcinoma. These differences make it feasible to validate microarray data by immunohistochemistry, and they will ultimately allow us to identify tumors according to their immunohistochemical phenotype. The accuracy of classifying endometrial tumors using a system based on their gene expression patterns is much higher than the accuracy of the FIGO grading system. Thus, this gene expression pattern-based system may prove to be crucial in developing novel treatment strategies for endometrial cancers at the molecular level in future. (C) 2011 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 20090001110085
项目资助者: Research Fund for the Doctoral Program of Higher Education of China
WOS记录号: WOS:000293548700009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62071
Appears in Collections:北京大学第二临床医学院_病理科_期刊论文

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作者单位: 1.Peking Univ Peoples Hosp, Dept Obstet & Gynecol, Beijing 100044, Peoples R China
2.Peking Univ Peoples Hosp, Dept Pathol, Beijing 100044, Peoples R China
3.Capital Bio Corp, Beijing 102206, Peoples R China

Recommended Citation:
Chen, Yonghua,Yao, Yuanyang,Zhang, Lili,et al. cDNA microarray analysis and immunohistochemistry reveal a distinct molecular phenotype in serous endometrial cancer compared to endometrioid endometrial cancer[J]. EXPERIMENTAL AND MOLECULAR PATHOLOGY,2011,91(1):373-384.
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