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学科主题: 口腔医学
题名:
Effect of skimmed pasteurized milk and Hank′s balanced salt solution on viability and osteogenic differentiation of human periodontal ligament stem cells
作者: Wang, Wen-Jun; Zhao, Yu-Ming; Feng, Xiao-Yu; Jia, Wei-Qian; Ge, Li-Hong
关键词: cell viability ; osteogenic differentiation ; periodontal ligament stem cells ; storage medium ; tooth avulsion
刊名: DENTAL TRAUMATOLOGY
发表日期: 2013-10-01
DOI: 10.1111/edt.12007
卷: 29, 期:5, 页:365-371
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: IN-VITRO VIABILITY ; GROWTH-FACTORS ; STORAGE MEDIA ; AVULSED TEETH ; FIBROBLAST VIABILITY ; CLONOGENIC CAPACITY ; TOOTH REPLANTATION ; TRANSPORT ; REGENERATION ; MITOGENICITY
英文摘要:

Background/AimThe purpose of this study was to compare the effect of skimmed pasteurized milk and Hank′s balanced salt solution on the viability and osteogenic differentiation potential of the human periodontal ligament stem cells at room temperature in vitro.

Material and methodsHuman periodontal ligament stem cells were obtained from extracted healthy third molars and conserved in skimmed pasteurized milk and Hank′s balanced salt solution for 1, 2, and 4h at room temperature to detect the viability of the cells and their osteogenic differentiation potential.

ResultsThe efficacy of skimmed pasteurized milk on cell viability at 4h was significantly higher than that of HBSS (P<0.05), and cells stored in skimmed pasteurized milk showed significantly higher levels of mineralization than those in HBSS at 2 and 4h (P<0.05).

ConclusionsSkimmed pasteurized milk was more effective than Hank′s balanced salt solution in maintaining the viability and osteogenic differentiation potential of PDLSCs at room temperature in vitro.

语种: 英语
所属项目编号: 81170928 ; 81141011
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000324474200004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62082
Appears in Collections:北京大学口腔医学院_儿童口腔科_期刊论文

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作者单位: Peking Univ, Dept Pediat Dent, Sch & Hosp Stomatol, Beijing 100871, Peoples R China

Recommended Citation:
Wang, Wen-Jun,Zhao, Yu-Ming,Feng, Xiao-Yu,et al. Effect of skimmed pasteurized milk and Hank&prime;s balanced salt solution on viability and osteogenic differentiation of human periodontal ligament stem cells[J]. DENTAL TRAUMATOLOGY,2013,29(5):365-371.
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