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学科主题口腔医学
Effect of skimmed pasteurized milk and Hank′s balanced salt solution on viability and osteogenic differentiation of human periodontal ligament stem cells
Wang, Wen-Jun; Zhao, Yu-Ming; Feng, Xiao-Yu; Jia, Wei-Qian; Ge, Li-Hong
关键词cell viability osteogenic differentiation periodontal ligament stem cells storage medium tooth avulsion
刊名DENTAL TRAUMATOLOGY
2013-10-01
DOI10.1111/edt.12007
29期:5页:365-371
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]IN-VITRO VIABILITY ; GROWTH-FACTORS ; STORAGE MEDIA ; AVULSED TEETH ; FIBROBLAST VIABILITY ; CLONOGENIC CAPACITY ; TOOTH REPLANTATION ; TRANSPORT ; REGENERATION ; MITOGENICITY
英文摘要

Background/AimThe purpose of this study was to compare the effect of skimmed pasteurized milk and Hank′s balanced salt solution on the viability and osteogenic differentiation potential of the human periodontal ligament stem cells at room temperature in vitro.

Material and methodsHuman periodontal ligament stem cells were obtained from extracted healthy third molars and conserved in skimmed pasteurized milk and Hank′s balanced salt solution for 1, 2, and 4h at room temperature to detect the viability of the cells and their osteogenic differentiation potential.

ResultsThe efficacy of skimmed pasteurized milk on cell viability at 4h was significantly higher than that of HBSS (P<0.05), and cells stored in skimmed pasteurized milk showed significantly higher levels of mineralization than those in HBSS at 2 and 4h (P<0.05).

ConclusionsSkimmed pasteurized milk was more effective than Hank′s balanced salt solution in maintaining the viability and osteogenic differentiation potential of PDLSCs at room temperature in vitro.

语种英语
WOS记录号WOS:000324474200004
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62082
专题北京大学口腔医学院_儿童口腔科
作者单位Peking Univ, Dept Pediat Dent, Sch & Hosp Stomatol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Wang, Wen-Jun,Zhao, Yu-Ming,Feng, Xiao-Yu,et al. Effect of skimmed pasteurized milk and Hank&prime;s balanced salt solution on viability and osteogenic differentiation of human periodontal ligament stem cells[J]. DENTAL TRAUMATOLOGY,2013,29(5):365-371.
APA Wang, Wen-Jun,Zhao, Yu-Ming,Feng, Xiao-Yu,Jia, Wei-Qian,&Ge, Li-Hong.(2013).Effect of skimmed pasteurized milk and Hank′s balanced salt solution on viability and osteogenic differentiation of human periodontal ligament stem cells.DENTAL TRAUMATOLOGY,29(5),365-371.
MLA Wang, Wen-Jun,et al."Effect of skimmed pasteurized milk and Hank′s balanced salt solution on viability and osteogenic differentiation of human periodontal ligament stem cells".DENTAL TRAUMATOLOGY 29.5(2013):365-371.
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