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学科主题: 临床医学
题名:
Recombinant human PDCD5 sensitizes chondrosarcomas to cisplatin chemotherapy in vitro and in vivo
作者: Chen, Changbao1; Zhou, Hua1; Xu, Lanjun2,3; Xu, Dong2,3; Wang, Ying2,3; Zhang, Yingmei2,3; Liu, Xiaoguang1; Liu, Zhongjun1; Ma, Dalong2,3; Ma, Qingjun1; Chen, Yingyu2,3
关键词: PDCD5 ; Apoptosis ; Chondrosarcoma ; Cisplatin ; Antitumor activity
刊名: APOPTOSIS
发表日期: 2010-07-01
DOI: 10.1007/s10495-010-0489-5
卷: 15, 期:7, 页:805-813
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: GENE-EXPRESSION PROFILES ; INDUCED APOPTOSIS ; TUMOR-CELLS ; MYELOID-LEUKEMIA ; CANCER ; DEATH ; BCL-2 ; OVEREXPRESSION ; OSTEOSARCOMA ; PROGRESSION
英文摘要:

Clinical management of chondrosarcoma remains a challenging problem, largely due to the toxicity and resistance of this tumor to conventional chemotherapy. Programmed Cell Death 5 (PDCD5) is a protein that accelerates apoptosis in different cell types in response to various stimuli, and has been shown to be down-regulated in many cancer tissues. In this study, mRNA and protein levels of PDCD5 were found to be up-regulated in cisplatin-treated SW1353 chondrosarcoma cells compared with untreated cells. Recombinant human PDCD5 (rhPDCD5) was also shown to sensitize chondrosarcoma cells to cisplatin-based chemotherapy, with inhibition of cell growth and apoptosis detected both in vitro and in vivo. Increased expression of Bax and decreased expression of Bcl-2 were also observed, along with release of cytochrome c from mitochondria into the cytosol. Additionally, cleavage of caspase-9 and caspase-3, as well as the cleavage of poly (ADP-ribose) polymerase (PARP), were detected, suggesting that sensitization of chondrosarcoma cells involves the intrinsic mitochondrial apoptosis pathway. In vivo, the treatment of a xenograft model of chondrosarcoma with rhPDCD5 and cisplatin significantly inhibited tumor cell proliferation and induced apoptosis compared to treatment with cisplatin alone. Overall, these data provide a theoretical basis for the administration of rhPDCD5 and cisplatin for the treatment of patients with chondrosarcoma.

语种: 英语
所属项目编号: 30871263 ; 30571870 ; 2009ZX09102-242 ; 2006AA02A305
项目资助者: National Natural Sciences Foundation of China ; National Key New Drug Creation Program of China ; National High Technology Research and Development Program of China
WOS记录号: WOS:000279190700005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62096
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Orthopaed, Beijing 100191, Peoples R China
2.Peking Univ, Ctr Human Dis Genom, Beijing 100191, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100191, Peoples R China

Recommended Citation:
Chen, Changbao,Zhou, Hua,Xu, Lanjun,et al. Recombinant human PDCD5 sensitizes chondrosarcomas to cisplatin chemotherapy in vitro and in vivo[J]. APOPTOSIS,2010,15(7):805-813.
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