|Differentiation of BMMSCs into odontoblast-like cells induced by natural dentine matrix|
|Lei, Gang1,2; Gang, Yan1,2; Jiang, Yujiao1,2; Wang, Sainan3; Yan, Ming1,2; Smith, Anthony J.4; Smith, Gay4; Cooper, Paul R.4; Tang, Chunbo1,5; Zhang, Guangdong1,2; Yu, Jinhua1,2|
|关键词||Bone marrow mesenchymal stem cells Dentine Dental pulp Odontoblast|
|刊名||ARCHIVES OF ORAL BIOLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Dentistry, Oral Surgery & Medicine|
|研究领域[WOS]||Dentistry, Oral Surgery & Medicine|
|关键词[WOS]||MESENCHYMAL STEM-CELLS ; EXFOLIATED DECIDUOUS TEETH ; FACTOR BETA-1 TGF-BETA-1 ; IN-VITRO ; BONE-MARROW ; REGENERATIVE MEDICINE ; EXTRACELLULAR-MATRIX ; CALCIUM HYDROXIDE ; APICAL PAPILLA ; PULP CELLS|
Objective: To assess the odontogenic potential of bone marrow mesenchymal stem cells (BMMSCs) to differentiate into odontoblast-like cells under the morphogenetic influence of dentine matrix as a possible basis for new stem cell-mediated therapeutic approaches to pulp diseases.
Design: BMMSCs were harvested from the whole bone marrow and cells at passages 3-5 were used for subsequent experiments. For in vitro studies, 1 x 10(4) cells were seeded on the surface of dentine slabs and co-cultured for 2 weeks in 24-well plates, then fixed, decalcified, embedded in paraffin and serial sections were processed for analyses. Haematoxylin-eosin (HE) staining was used for the morphological analysis of BMMSCs on the dentine slabs. The protein expression of dentine sialoprotein (DSP) in co-cultured BMMSCs was detected by immunohistochemical (IHc) staining. For in vivo studies, 5 x 10(6) cells were collected as cell pellets, seeded onto dentine slices and transplanted into renal capsules for 6 weeks. Histological analyses of harvested tissues were performed as described for the in vitro studies. Total RNA and protein were extracted from harvested tissues and Dspp/DSP expression was investigated by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively.
Results: After 2 weeks of co-culture with dentine slabs, BMMSCs demonstrated good viability in terms of morphological appearance and some showed polarization and extension of their cytoplasmic processes into dentine tubules with DSP expression. In vivo study demonstrated similar morphological changes and DSP expression in cells adjacent to dentine. RT-PCR and Western blot also demonstrated that the expression of Dspp/DSP in the co-cultured BMMSCs groups was higher than in the control groups.
Conclusion: Dentine Matrix can signal morphogenic induction of differentiation of BMMSCs into odontoblast-like cells in vivo and in vitro. (C) 2013 Elsevier Ltd. All rights reserved.
|项目编号||52008202 ; RC2011140 ; 2011NJMU102|
|资助机构||"355" Project of Stomatological Hospital of Jiangsu Province ; Medical Elitist Project of Jiangsu Province ; Science Development Fund of Nanjing Medical University ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)|
|作者单位||1.Nanjing Med Univ, Inst Stomatol, Nanjing 210029, Jiangsu, Peoples R China|
2.Nanjing Med Univ, Sch Stomatol, Dept Conservat Dent & Endodont, Nanjing 210029, Jiangsu, Peoples R China
3.Peking Univ, Sch Stomatol, Dept Conservat Dent & Endodont, Beijing 100081, Peoples R China
4.Univ Birmingham, Sch Dent, Birmingham B4 6NN, W Midlands, England
5.Nanjing Med Univ, Sch Stornatol, Dept Prosthet Dent, Nanjing 210029, Jiangsu, Peoples R China
|Lei, Gang,Gang, Yan,Jiang, Yujiao,et al. Differentiation of BMMSCs into odontoblast-like cells induced by natural dentine matrix[J]. ARCHIVES OF ORAL BIOLOGY,2013,58(7):862-870.|
|APA||Lei, Gang.,Gang, Yan.,Jiang, Yujiao.,Wang, Sainan.,Yan, Ming.,...&Yu, Jinhua.(2013).Differentiation of BMMSCs into odontoblast-like cells induced by natural dentine matrix.ARCHIVES OF ORAL BIOLOGY,58(7),862-870.|
|MLA||Lei, Gang,et al."Differentiation of BMMSCs into odontoblast-like cells induced by natural dentine matrix".ARCHIVES OF ORAL BIOLOGY 58.7(2013):862-870.|