IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Differentiation of BMMSCs into odontoblast-like cells induced by natural dentine matrix
Lei, Gang1,2; Gang, Yan1,2; Jiang, Yujiao1,2; Wang, Sainan3; Yan, Ming1,2; Smith, Anthony J.4; Smith, Gay4; Cooper, Paul R.4; Tang, Chunbo1,5; Zhang, Guangdong1,2; Yu, Jinhua1,2
关键词Bone marrow mesenchymal stem cells Dentine Dental pulp Odontoblast
刊名ARCHIVES OF ORAL BIOLOGY
2013-07-01
DOI10.1016/j.archoralbio.2013.01.002
58期:7页:862-870
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
资助者"355" Project of Stomatological Hospital of Jiangsu Province ; Medical Elitist Project of Jiangsu Province ; Science Development Fund of Nanjing Medical University ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; "355" Project of Stomatological Hospital of Jiangsu Province ; Medical Elitist Project of Jiangsu Province ; Science Development Fund of Nanjing Medical University ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]MESENCHYMAL STEM-CELLS ; EXFOLIATED DECIDUOUS TEETH ; FACTOR BETA-1 TGF-BETA-1 ; IN-VITRO ; BONE-MARROW ; REGENERATIVE MEDICINE ; EXTRACELLULAR-MATRIX ; CALCIUM HYDROXIDE ; APICAL PAPILLA ; PULP CELLS
英文摘要

Objective: To assess the odontogenic potential of bone marrow mesenchymal stem cells (BMMSCs) to differentiate into odontoblast-like cells under the morphogenetic influence of dentine matrix as a possible basis for new stem cell-mediated therapeutic approaches to pulp diseases.

Design: BMMSCs were harvested from the whole bone marrow and cells at passages 3-5 were used for subsequent experiments. For in vitro studies, 1 x 10(4) cells were seeded on the surface of dentine slabs and co-cultured for 2 weeks in 24-well plates, then fixed, decalcified, embedded in paraffin and serial sections were processed for analyses. Haematoxylin-eosin (HE) staining was used for the morphological analysis of BMMSCs on the dentine slabs. The protein expression of dentine sialoprotein (DSP) in co-cultured BMMSCs was detected by immunohistochemical (IHc) staining. For in vivo studies, 5 x 10(6) cells were collected as cell pellets, seeded onto dentine slices and transplanted into renal capsules for 6 weeks. Histological analyses of harvested tissues were performed as described for the in vitro studies. Total RNA and protein were extracted from harvested tissues and Dspp/DSP expression was investigated by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively.

Results: After 2 weeks of co-culture with dentine slabs, BMMSCs demonstrated good viability in terms of morphological appearance and some showed polarization and extension of their cytoplasmic processes into dentine tubules with DSP expression. In vivo study demonstrated similar morphological changes and DSP expression in cells adjacent to dentine. RT-PCR and Western blot also demonstrated that the expression of Dspp/DSP in the co-cultured BMMSCs groups was higher than in the control groups.

Conclusion: Dentine Matrix can signal morphogenic induction of differentiation of BMMSCs into odontoblast-like cells in vivo and in vitro. (C) 2013 Elsevier Ltd. All rights reserved.

语种英语
所属项目编号52008202 ; RC2011140 ; 2011NJMU102
资助者"355" Project of Stomatological Hospital of Jiangsu Province ; Medical Elitist Project of Jiangsu Province ; Science Development Fund of Nanjing Medical University ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; "355" Project of Stomatological Hospital of Jiangsu Province ; Medical Elitist Project of Jiangsu Province ; Science Development Fund of Nanjing Medical University ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
WOS记录号WOS:000320417500014
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62108
专题北京大学口腔医学院
作者单位1.Nanjing Med Univ, Inst Stomatol, Nanjing 210029, Jiangsu, Peoples R China
2.Nanjing Med Univ, Sch Stomatol, Dept Conservat Dent & Endodont, Nanjing 210029, Jiangsu, Peoples R China
3.Peking Univ, Sch Stomatol, Dept Conservat Dent & Endodont, Beijing 100081, Peoples R China
4.Univ Birmingham, Sch Dent, Birmingham B4 6NN, W Midlands, England
5.Nanjing Med Univ, Sch Stornatol, Dept Prosthet Dent, Nanjing 210029, Jiangsu, Peoples R China
推荐引用方式
GB/T 7714
Lei, Gang,Gang, Yan,Jiang, Yujiao,et al. Differentiation of BMMSCs into odontoblast-like cells induced by natural dentine matrix[J]. ARCHIVES OF ORAL BIOLOGY,2013,58(7):862-870.
APA Lei, Gang.,Gang, Yan.,Jiang, Yujiao.,Wang, Sainan.,Yan, Ming.,...&Yu, Jinhua.(2013).Differentiation of BMMSCs into odontoblast-like cells induced by natural dentine matrix.ARCHIVES OF ORAL BIOLOGY,58(7),862-870.
MLA Lei, Gang,et al."Differentiation of BMMSCs into odontoblast-like cells induced by natural dentine matrix".ARCHIVES OF ORAL BIOLOGY 58.7(2013):862-870.
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