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学科主题: 临床医学
题名:
Alteration of cadherin isoform expression and inhibition of gap junctions in stomach carcinoma cells
作者: Li, JP; Zhang, ZQ; Ning, T; Ke, Y; Lin, ZX
关键词: E-cadherin ; N-cadherin ; isoform alteration ; Cx32 ; stomach cancer
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2001-12-01
卷: 46, 期:24, 页:2068-2073
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: ADHESION ; CANCER ; DEDIFFERENTIATION ; MORPHOGENESIS ; COMMUNICATION ; GENE
英文摘要:

To explore cell malignant phenotype correlated changes of cell surface adhesion molecules and cell-cell communication in carcinogenesis, human stomach transformed and cancer cell lines were investigated. Expressions of E-cadherin, N-cadherin, alpha-catenin, beta-catenin as well as gap junction (GJ) protein Cx32 were studied by utilization of immunoblotting, immunocytochemical and fluorescent dye transfer methods. Mammalian normal stomach mucosal cells expressed E-cadherin but not N-cadherin. E-cadherin immunofluorescence was detected at cell membranous adherens junctions (AJ) where colocalization with immunofluorescent staining of inner surface adhesion plaque proteins alpha- and beta-catenins was observed. The existence of E-cadherin/catenin (alpha-, beta-) protein complexes as AJ was suggested. In transformed and stomach cancer cells E-cadherin was inhibited, instead, N-cadherin was expressed and localized at membranous AJ where co-staining with alpha- and beta-catenin fluorescence was observed. Formation of N-cadherin/catenin (alpha-, beta-) protein complex at AJs of transformed and cancer cells was suggested. The above observations were further supported by immunoblotting results. Normal stomach muscosal and transformed cells expressed Cx32 at membranous GJ and were competent of gap junction communication (GJIC). In stomach cancer cells, Cx32 was inhibited and GJIC was defective. The results suggested that changes of signal pathways mediated by both cell adhesion and cell communication systems are associated intracellular events of stomach carcinogenesis. The alteration of cadherin isoform from E- to N-cadherin in transformed and stomach cancer cells is the first report.

语种: 英语
WOS记录号: WOS:000173323300012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62114
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Peking Univ, Sch Oncol, Beijing 100034, Peoples R China
2.Peking Univ, Beijing Inst Canc Res, Beijing 100034, Peoples R China

Recommended Citation:
Li, JP,Zhang, ZQ,Ning, T,et al. Alteration of cadherin isoform expression and inhibition of gap junctions in stomach carcinoma cells[J]. CHINESE SCIENCE BULLETIN,2001,46(24):2068-2073.
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