IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Alteration of cadherin isoform expression and inhibition of gap junctions in stomach carcinoma cells
Li, JP; Zhang, ZQ; Ning, T; Ke, Y; Lin, ZX
关键词E-cadherin N-cadherin isoform alteration Cx32 stomach cancer
刊名CHINESE SCIENCE BULLETIN
2001-12-01
46期:24页:2068-2073
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]ADHESION ; CANCER ; DEDIFFERENTIATION ; MORPHOGENESIS ; COMMUNICATION ; GENE
英文摘要

To explore cell malignant phenotype correlated changes of cell surface adhesion molecules and cell-cell communication in carcinogenesis, human stomach transformed and cancer cell lines were investigated. Expressions of E-cadherin, N-cadherin, alpha-catenin, beta-catenin as well as gap junction (GJ) protein Cx32 were studied by utilization of immunoblotting, immunocytochemical and fluorescent dye transfer methods. Mammalian normal stomach mucosal cells expressed E-cadherin but not N-cadherin. E-cadherin immunofluorescence was detected at cell membranous adherens junctions (AJ) where colocalization with immunofluorescent staining of inner surface adhesion plaque proteins alpha- and beta-catenins was observed. The existence of E-cadherin/catenin (alpha-, beta-) protein complexes as AJ was suggested. In transformed and stomach cancer cells E-cadherin was inhibited, instead, N-cadherin was expressed and localized at membranous AJ where co-staining with alpha- and beta-catenin fluorescence was observed. Formation of N-cadherin/catenin (alpha-, beta-) protein complex at AJs of transformed and cancer cells was suggested. The above observations were further supported by immunoblotting results. Normal stomach muscosal and transformed cells expressed Cx32 at membranous GJ and were competent of gap junction communication (GJIC). In stomach cancer cells, Cx32 was inhibited and GJIC was defective. The results suggested that changes of signal pathways mediated by both cell adhesion and cell communication systems are associated intracellular events of stomach carcinogenesis. The alteration of cadherin isoform from E- to N-cadherin in transformed and stomach cancer cells is the first report.

语种英语
WOS记录号WOS:000173323300012
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62114
专题北京大学临床肿瘤学院
作者单位1.Peking Univ, Sch Oncol, Beijing 100034, Peoples R China
2.Peking Univ, Beijing Inst Canc Res, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Li, JP,Zhang, ZQ,Ning, T,et al. Alteration of cadherin isoform expression and inhibition of gap junctions in stomach carcinoma cells[J]. CHINESE SCIENCE BULLETIN,2001,46(24):2068-2073.
APA Li, JP,Zhang, ZQ,Ning, T,Ke, Y,&Lin, ZX.(2001).Alteration of cadherin isoform expression and inhibition of gap junctions in stomach carcinoma cells.CHINESE SCIENCE BULLETIN,46(24),2068-2073.
MLA Li, JP,et al."Alteration of cadherin isoform expression and inhibition of gap junctions in stomach carcinoma cells".CHINESE SCIENCE BULLETIN 46.24(2001):2068-2073.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, JP]的文章
[Zhang, ZQ]的文章
[Ning, T]的文章
百度学术
百度学术中相似的文章
[Li, JP]的文章
[Zhang, ZQ]的文章
[Ning, T]的文章
必应学术
必应学术中相似的文章
[Li, JP]的文章
[Zhang, ZQ]的文章
[Ning, T]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。