IR@PKUHSC  > 基础医学院  > 病原生物学系
学科主题基础医学
Recombinant Lysostaphin Protects Mice from Methicillin-Resistant Staphylococcus aureus Pneumonia
Chen, Chen1,2,3,4; Fan, Huahao1,5; Huang, Yong1; Peng, Fan1; Fan, Hang1; Yuan, Shoujun2; Tong, Yigang1
刊名BIOMED RESEARCH INTERNATIONAL
2014
DOI10.1155/2014/602185
4
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
资助者National Natural Science Foundation of China ; National Hi-Tech Research and Development (863) Program of China ; China Mega-Project on Major Drug Development ; China Mega-Project on Infectious Disease Prevention ; State Key Laboratory of Pathogen and BioSecurity Program ; National Natural Science Foundation of China ; National Hi-Tech Research and Development (863) Program of China ; China Mega-Project on Major Drug Development ; China Mega-Project on Infectious Disease Prevention ; State Key Laboratory of Pathogen and BioSecurity Program
研究领域[WOS]Biotechnology & Applied Microbiology ; Research & Experimental Medicine
关键词[WOS]PANTON-VALENTINE LEUKOCIDIN ; PEPTIDOGLYCAN INTERPEPTIDE BRIDGE ; INFECTIONS ; BACTEREMIA ; MORTALITY ; VANCOMYCIN ; STRAINS ; RABBIT ; VITRO ; FEMA
英文摘要

The advent of methicillin-resistant Staphylococcus aureus (MRSA) and the frequent and excessive abuse of ventilators have made MRSA pneumonia an inordinate threat to human health. Appropriate antibacterial therapies are crucial, including the use of lysostaphin as an alternative to antibiotics. To explore the potential use of lysostaphin as a therapeutic agent for MRSA pneumonia, mice were intranasally infected with MRSA and then treated with recombinant lysostaphin (rLys; 45mg/kg in the high-dose group and 1mg/kg in the low-dose group) (0.33mg/mL, 15mg/mL), vancomycin (120mg/kg) (40mg/mL), or phosphate-buffered saline (PBS, negative control) 4 h after infection. Therapeutic efficacy was assessed by mouse survival, lung histopathology, bacterial density in the lungs, bodyweight, lung weight, temperature, white blood cells counts, lymphocytes counts, granulocytes counts, and monocytes counts. The mice treated with rLys showed lower mortality, less lung parenchymal damage, and lower bacterial density at metastatic tissue sites than mice treated with PBS or vancomycin. The overall mortality was 100%, 60%, 40%, and 60% for the control, vancomycin, high-dose rLys, and low-dose rLys groups, respectively. These findings indicate that, as a therapeutic agent for MRSA pneumonia, lysostaphin exerts profound protective effects in mice against the morbidity and mortality associated with S. aureus pneumonia.

语种英语
所属项目编号81072350 ; 2012AA022-003 ; 2011ZX09401-023 ; 2013ZX10004-605 ; 2011ZX10004-001 ; SKLPBS1113
资助者National Natural Science Foundation of China ; National Hi-Tech Research and Development (863) Program of China ; China Mega-Project on Major Drug Development ; China Mega-Project on Infectious Disease Prevention ; State Key Laboratory of Pathogen and BioSecurity Program ; National Natural Science Foundation of China ; National Hi-Tech Research and Development (863) Program of China ; China Mega-Project on Major Drug Development ; China Mega-Project on Infectious Disease Prevention ; State Key Laboratory of Pathogen and BioSecurity Program
WOS记录号WOS:000339744000001
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62123
专题基础医学院_病原生物学系
作者单位1.Anhui Med Univ, Hefei 230032, Peoples R China
2.Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China
3.Beijing Inst Radiat Med, Dept Pharmacol & Toxicol, Beijing 100850, Peoples R China
4.Univ Tsukuba, Lab Gene Regulat, Fac Med, Tsukuba, Ibaraki 3058575, Japan
5.Peking Univ, Dept Microbiol, Hlth Sci Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Chen, Chen,Fan, Huahao,Huang, Yong,et al. Recombinant Lysostaphin Protects Mice from Methicillin-Resistant Staphylococcus aureus Pneumonia[J]. BIOMED RESEARCH INTERNATIONAL,2014(4).
APA Chen, Chen.,Fan, Huahao.,Huang, Yong.,Peng, Fan.,Fan, Hang.,...&Tong, Yigang.(2014).Recombinant Lysostaphin Protects Mice from Methicillin-Resistant Staphylococcus aureus Pneumonia.BIOMED RESEARCH INTERNATIONAL(4).
MLA Chen, Chen,et al."Recombinant Lysostaphin Protects Mice from Methicillin-Resistant Staphylococcus aureus Pneumonia".BIOMED RESEARCH INTERNATIONAL .4(2014).
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Recombinant Lysostap(1599KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Chen, Chen]的文章
[Fan, Huahao]的文章
[Huang, Yong]的文章
百度学术
百度学术中相似的文章
[Chen, Chen]的文章
[Fan, Huahao]的文章
[Huang, Yong]的文章
必应学术
必应学术中相似的文章
[Chen, Chen]的文章
[Fan, Huahao]的文章
[Huang, Yong]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Recombinant Lysostaphin Protects Mice from Methicillin-Resistant Staphylococcus aureus Pneumonia.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。