北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 心血管所  > 期刊论文
学科主题: 基础医学
题名:
Insufficient radiofrequency ablation promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells through Akt and ERK signaling pathways
作者: Dong, Shuying1; Kong, Jian1; Kong, Fandong1; Kong, Jinge5,6; Gao, Jun1; Ke, Shan1; Wang, Shaohong1; Ding, Xuemei1; Sun, Wenbing1; Zheng, Lemin2,3,4
关键词: Insufficient radiofrequency ablation ; Epithelial-mesenchymal transition ; Hepatocellular carcinoma ; Metastasis
刊名: JOURNAL OF TRANSLATIONAL MEDICINE
发表日期: 2013-10-29
DOI: 10.1186/1479-5876-11-273
卷: 11
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: COLORECTAL LIVER METASTASES ; CANCER CELLS ; EMT ; PROGRESSION ; ACTIVATION ; INVASION ; MIGRATION ; OUTGROWTH ; ANOIKIS
英文摘要:

Background: Residual tumor progression after insufficient radiofrequency ablation (RFA) has been recently reported. However, whether epithelial-mesenchymal transition (EMT), which is a key process that drives cancer metastasis, is involved in the tumor progression after insufficient RFA is not well understood.

Methods: Human hepatocellular carcinoma (HCC) cell lines SMMC7721 and Huh7 were used. Insufficient RFA was simulated using a water bath (47 degrees C 5 min, 10 min, 15 min, 20 min and 25 min gradually). MTT assay was used to evaluate the proliferation of HCC cells in vitro. Migration and invasion of HCC cells were determined by transwell assay. The molecular changes in HCC cells after insufficient RFA were evaluated by western blot. LY294002 and PD98059 were used to treat HCC cells. An ectopic nude mice model and a tail vein metastatic assay were used to evaluate the growth and metastatic potential of SMMC7721 cells in vivo after insufficient RFA.

Results: SMMC7721 and Huh7 cells after insufficient RFA (named as SMMC7721-H and Huh7-H respectively) exhibited enhanced proliferation, migration and invasion (6.4% and 23.6%, 33.2% and 66.1%, and 44.1% and 57.4% increase respectively) in vitro. Molecular changes of EMT were observed in SMMC7721-H and Huh7-H cells. LY294002 and PD98059 inhibited the EMT of SMMC7721-H and Huh7-H cells. SMMC7721-H cells also exhibited larger tumor size (1440.8 +/- 250.3 mm(3) versus 1048.56 +/- 227.6 mm(3)) and more lung metastasis (97.4% increase) than SMMC7721 cells in vivo. Higher expression of PCNA, N-cadherin and MMP-2 and MMP-9, was also observed in SMMC7721-H tumors.

Conclusions: Insufficient RFA could directly promote the invasiveness and metastasis of HCC cells. Insufficient RFA may promote the EMT of HCC cells through Akt and ERK signaling pathways.

语种: 英语
所属项目编号: 320675007131 ; 32067501207 ; 1300171711 ; 2009-3-11 ; 2010CB912504 ; 2011CB503900 ; 81170101 ; 30821001 ; 81370235 ; 7122106
项目资助者: Dr. Jieping Wu Medical Foundation ; Clinical-Basic Medicine Cooperation Fund of Capital Medical University ; Program for Medical Academic backbone (Beijing, China) ; "973" National ST Major Project ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing, China
WOS记录号: WOS:000329400400001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62130
Appears in Collections:基础医学院_心血管所_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
art%3A10.1186%2F1479-5876-11-273.pdf(5232KB)期刊论文作者接受稿限制开放 联系获取全文

作者单位: 1.Capital Med Univ, Beijing Chaoyang Hosp, Dept Hepatobiliary Surg, Beijing 100043, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Inst Cardiovasc Sci, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Inst Syst Biomed,Key Lab Mol Cardiovasc,Educ Mini, Beijing 100191, Peoples R China
4.Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Beijing 100191, Peoples R China
5.Peking Univ, Natl Hlth & Family Planning Commiss, Minist Educ, Key Lab Neurosci,Sch Basic Med Sci,Neurosci Res I, Beijing 100191, Peoples R China
6.Peking Univ, Natl Hlth & Family Planning Commiss, Minist Educ, Key Lab Neurosci,Sch Basic Med Sci,Dept Neurobiol, Beijing 100191, Peoples R China

Recommended Citation:
Dong, Shuying,Kong, Jian,Kong, Fandong,et al. Insufficient radiofrequency ablation promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells through Akt and ERK signaling pathways[J]. JOURNAL OF TRANSLATIONAL MEDICINE,2013,11.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Dong, Shuying]'s Articles
[Kong, Jian]'s Articles
[Kong, Fandong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Dong, Shuying]‘s Articles
[Kong, Jian]‘s Articles
[Kong, Fandong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace