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学科主题基础医学
Insufficient radiofrequency ablation promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells through Akt and ERK signaling pathways
Dong, Shuying1; Kong, Jian1; Kong, Fandong1; Kong, Jinge5,6; Gao, Jun1; Ke, Shan1; Wang, Shaohong1; Ding, Xuemei1; Sun, Wenbing1; Zheng, Lemin2,3,4
关键词Insufficient radiofrequency ablation Epithelial-mesenchymal transition Hepatocellular carcinoma Metastasis
刊名JOURNAL OF TRANSLATIONAL MEDICINE
2013-10-29
DOI10.1186/1479-5876-11-273
11
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental
研究领域[WOS]Research & Experimental Medicine
关键词[WOS]COLORECTAL LIVER METASTASES ; CANCER CELLS ; EMT ; PROGRESSION ; ACTIVATION ; INVASION ; MIGRATION ; OUTGROWTH ; ANOIKIS
英文摘要

Background: Residual tumor progression after insufficient radiofrequency ablation (RFA) has been recently reported. However, whether epithelial-mesenchymal transition (EMT), which is a key process that drives cancer metastasis, is involved in the tumor progression after insufficient RFA is not well understood.

Methods: Human hepatocellular carcinoma (HCC) cell lines SMMC7721 and Huh7 were used. Insufficient RFA was simulated using a water bath (47 degrees C 5 min, 10 min, 15 min, 20 min and 25 min gradually). MTT assay was used to evaluate the proliferation of HCC cells in vitro. Migration and invasion of HCC cells were determined by transwell assay. The molecular changes in HCC cells after insufficient RFA were evaluated by western blot. LY294002 and PD98059 were used to treat HCC cells. An ectopic nude mice model and a tail vein metastatic assay were used to evaluate the growth and metastatic potential of SMMC7721 cells in vivo after insufficient RFA.

Results: SMMC7721 and Huh7 cells after insufficient RFA (named as SMMC7721-H and Huh7-H respectively) exhibited enhanced proliferation, migration and invasion (6.4% and 23.6%, 33.2% and 66.1%, and 44.1% and 57.4% increase respectively) in vitro. Molecular changes of EMT were observed in SMMC7721-H and Huh7-H cells. LY294002 and PD98059 inhibited the EMT of SMMC7721-H and Huh7-H cells. SMMC7721-H cells also exhibited larger tumor size (1440.8 +/- 250.3 mm(3) versus 1048.56 +/- 227.6 mm(3)) and more lung metastasis (97.4% increase) than SMMC7721 cells in vivo. Higher expression of PCNA, N-cadherin and MMP-2 and MMP-9, was also observed in SMMC7721-H tumors.

Conclusions: Insufficient RFA could directly promote the invasiveness and metastasis of HCC cells. Insufficient RFA may promote the EMT of HCC cells through Akt and ERK signaling pathways.

语种英语
WOS记录号WOS:000329400400001
项目编号320675007131 ; 32067501207 ; 1300171711 ; 2009-3-11 ; 2010CB912504 ; 2011CB503900 ; 81170101 ; 30821001 ; 81370235 ; 7122106
资助机构Dr. Jieping Wu Medical Foundation ; Clinical-Basic Medicine Cooperation Fund of Capital Medical University ; Program for Medical Academic backbone (Beijing, China) ; "973" National ST Major Project ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing, China
引用统计
被引频次:31[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62130
专题北京大学基础医学院_心血管所
北京大学基础医学院
作者单位1.Capital Med Univ, Beijing Chaoyang Hosp, Dept Hepatobiliary Surg, Beijing 100043, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Inst Cardiovasc Sci, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Inst Syst Biomed,Key Lab Mol Cardiovasc,Educ Mini, Beijing 100191, Peoples R China
4.Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Beijing 100191, Peoples R China
5.Peking Univ, Natl Hlth & Family Planning Commiss, Minist Educ, Key Lab Neurosci,Sch Basic Med Sci,Neurosci Res I, Beijing 100191, Peoples R China
6.Peking Univ, Natl Hlth & Family Planning Commiss, Minist Educ, Key Lab Neurosci,Sch Basic Med Sci,Dept Neurobiol, Beijing 100191, Peoples R China
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GB/T 7714
Dong, Shuying,Kong, Jian,Kong, Fandong,et al. Insufficient radiofrequency ablation promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells through Akt and ERK signaling pathways[J]. JOURNAL OF TRANSLATIONAL MEDICINE,2013,11.
APA Dong, Shuying.,Kong, Jian.,Kong, Fandong.,Kong, Jinge.,Gao, Jun.,...&Zheng, Lemin.(2013).Insufficient radiofrequency ablation promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells through Akt and ERK signaling pathways.JOURNAL OF TRANSLATIONAL MEDICINE,11.
MLA Dong, Shuying,et al."Insufficient radiofrequency ablation promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells through Akt and ERK signaling pathways".JOURNAL OF TRANSLATIONAL MEDICINE 11(2013).
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