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学科主题: 临床医学
题名:
Radiolabeling and evaluation of Cu-64-DOTA-F56 peptide targeting vascular endothelial growth factor receptor 1 in the molecular imaging of gastric cancer
作者: Zhu, Hua1; Zhao, Chuanke2; Liu, Fei1; Wang, Lixin2; Feng, Junnan2; Zhou, Zheng2; Qu, Like2; Shou, Chengchao2; Yang, Zhi1
关键词: Vascular endothelial growth factor receptor 1 ; copper-64 ; gastric cancer ; molecular imaging ; F56 peptide
刊名: AMERICAN JOURNAL OF CANCER RESEARCH
发表日期: 2015
卷: 5, 期:11, 页:3301-3310
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: TUMOR ANGIOGENESIS ; F-18-FDG PET ; VEGF ; EXPRESSION ; METASTASIS ; CARCINOMA ; GENE
英文摘要:

Noninvasive imaging of vascular endothelial growth factor receptor 1 (VEGFR1) remains a great challenge in early diagnosis of gastric cancer. Here we reported the synthesis, radiolabeling, and evaluation of a novel Cu-64-radiolabeled peptide for noninvasive positron emission tomography (PET) imaging of VEGFR1 positive gastric cancer. The binding of modified peptide WHSDMEWWYLLG (termed as F56) to VEGER-1 expressed in gastric cancer cell BCG823 has been confirmed by immune-fluorescence overlap. DOTA-F56 was designed and prepared by solid-phase synthesis and folded in vitro. Cu-64-DOTA-F56 was synthesized in high radiochemical yield and high specific activity (S.A. up to 255.6 GBq/mmol). It has excellent in vitro stability. Micro-PET imaging of 64Cu-DOTA-F56 identifies tumor in BCG823 tumor-bearing mice, while that of F-18-FDG does not. Immunohistochemical analysis of excised BCG823 xenograft showed colocalization between the PET images and the staining of VEGFR1. These results demonstrated that Cu-64-DOTA-F56 peptide has potential as a noninvasive imaging agent in VEGFR1 positive tumors.

语种: 英语
所属项目编号: 2012ZX09103-301-013 ; 2015CB553906 ; 81172083 ; 81371592 ; 81401467 ; 81301966 ; 81571705 ; 7132040 ; 7154188
项目资助者: Major Project on Drug Research and Development for the 12th Five-Year Plan of China ; National 973 Program of China ; National Natural Science Foundation of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000366698300003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62141
Appears in Collections:北京大学临床肿瘤学院_核医学科_期刊论文

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作者单位: 1.Peking Univ Canc Hosp & Inst, Dept Nucl Med, Beijing 100142, Peoples R China
2.Peking Univ Canc Hosp & Inst, Dept Biochem & Mol Biol, Key Lab Carcinogenesis & Translat Res, Minist Educ, Beijing 100142, Peoples R China

Recommended Citation:
Zhu, Hua,Zhao, Chuanke,Liu, Fei,et al. Radiolabeling and evaluation of Cu-64-DOTA-F56 peptide targeting vascular endothelial growth factor receptor 1 in the molecular imaging of gastric cancer[J]. AMERICAN JOURNAL OF CANCER RESEARCH,2015,5(11):3301-3310.
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