IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Hypersensitive mAChRs are Involved in the Epiphora of Transplanted Glands
Ding, C.1,2; Cong, X.3,4; Zhang, Y.3,4; Yang, N. Y.5; Li, S. L.1,2; Wu, L. L.3,4; Yu, G. Y.5
关键词aquaporin 5 transplantation lipid raft secretion muscarinic acetylcholine receptor submandibular gland
刊名JOURNAL OF DENTAL RESEARCH
2014-03-01
DOI10.1177/0022034513519107
93期:3页:306-312
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]AUTOLOGOUS SUBMANDIBULAR-GLAND ; KERATOCONJUNCTIVITIS SICCA ; SALIVARY-GLANDS ; WATER CHANNEL ; SJOGRENS-SYNDROME ; LIPID RAFTS ; CAVEOLAE ; MICRODOMAINS ; SECRETION ; IDENTIFICATION
英文摘要

Autologous transplantation of the submandibular gland is an effective treatment for severe dry eye syndrome. However, more than 40% of patients experience epiphora 3 to 6 months after transplantation. The underlying mechanism of epiphora remains to be elucidated. To investigate the potential roles of muscarinic acetylcholine receptors (mAChRs) in the induction of epiphora in transplanted glands, we assessed and found elevated mRNA and protein expression of M1- and M3-mAChR in transplanted glands from epiphora patients. The content of inositol 1, 4, 5-trisphosphate was also elevated. Moreover, carbachol (5 and 10 mu M) induced greater increase of [Ca2+](i) in isolated epiphora submandibular cells than in controls. Although aquaporin-5 (AQP5) content and distribution in the apical and lateral plasma of epiphora glands did not change, AQP5 content was reduced in lipid microdomains (lipid rafts and caveolae) but increased in non-lipid microdomains compared with controls. Carbachol (10 mu M) increased the ratio of non-lipid microdomain to total AQP5 in the cultured control submandibular gland tissue. Taken together, these results indicated that hypersensitive mAChRs might be involved in the epiphora of transplanted submandibular glands by modulating AQP5 trafficking.

语种英语
所属项目编号81200799 ; 81271161
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000331433200015
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62142
Collection北京大学口腔医学院
作者单位1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Ctr Lab, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Ctr Salivary Gland Dis, Beijing 100081, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
5.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Ding, C.,Cong, X.,Zhang, Y.,et al. Hypersensitive mAChRs are Involved in the Epiphora of Transplanted Glands[J]. JOURNAL OF DENTAL RESEARCH,2014,93(3):306-312.
APA Ding, C..,Cong, X..,Zhang, Y..,Yang, N. Y..,Li, S. L..,...&Yu, G. Y..(2014).Hypersensitive mAChRs are Involved in the Epiphora of Transplanted Glands.JOURNAL OF DENTAL RESEARCH,93(3),306-312.
MLA Ding, C.,et al."Hypersensitive mAChRs are Involved in the Epiphora of Transplanted Glands".JOURNAL OF DENTAL RESEARCH 93.3(2014):306-312.
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