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学科主题: 口腔医学
题名:
Hypersensitive mAChRs are Involved in the Epiphora of Transplanted Glands
作者: Ding, C.1,2; Cong, X.3,4; Zhang, Y.3,4; Yang, N. Y.5; Li, S. L.1,2; Wu, L. L.3,4; Yu, G. Y.5
关键词: aquaporin 5 ; transplantation ; lipid raft ; secretion ; muscarinic acetylcholine receptor ; submandibular gland
刊名: JOURNAL OF DENTAL RESEARCH
发表日期: 2014-03-01
DOI: 10.1177/0022034513519107
卷: 93, 期:3, 页:306-312
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: AUTOLOGOUS SUBMANDIBULAR-GLAND ; KERATOCONJUNCTIVITIS SICCA ; SALIVARY-GLANDS ; WATER CHANNEL ; SJOGRENS-SYNDROME ; LIPID RAFTS ; CAVEOLAE ; MICRODOMAINS ; SECRETION ; IDENTIFICATION
英文摘要:

Autologous transplantation of the submandibular gland is an effective treatment for severe dry eye syndrome. However, more than 40% of patients experience epiphora 3 to 6 months after transplantation. The underlying mechanism of epiphora remains to be elucidated. To investigate the potential roles of muscarinic acetylcholine receptors (mAChRs) in the induction of epiphora in transplanted glands, we assessed and found elevated mRNA and protein expression of M1- and M3-mAChR in transplanted glands from epiphora patients. The content of inositol 1, 4, 5-trisphosphate was also elevated. Moreover, carbachol (5 and 10 mu M) induced greater increase of [Ca2+](i) in isolated epiphora submandibular cells than in controls. Although aquaporin-5 (AQP5) content and distribution in the apical and lateral plasma of epiphora glands did not change, AQP5 content was reduced in lipid microdomains (lipid rafts and caveolae) but increased in non-lipid microdomains compared with controls. Carbachol (10 mu M) increased the ratio of non-lipid microdomain to total AQP5 in the cultured control submandibular gland tissue. Taken together, these results indicated that hypersensitive mAChRs might be involved in the epiphora of transplanted submandibular glands by modulating AQP5 trafficking.

语种: 英语
所属项目编号: 81200799 ; 81271161
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000331433200015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62142
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Ctr Lab, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Ctr Salivary Gland Dis, Beijing 100081, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
5.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China

Recommended Citation:
Ding, C.,Cong, X.,Zhang, Y.,et al. Hypersensitive mAChRs are Involved in the Epiphora of Transplanted Glands[J]. JOURNAL OF DENTAL RESEARCH,2014,93(3):306-312.
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