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Functional Diversity of the Microbial Community in Healthy Subjects and Periodontitis Patients Based on Sole Carbon Source Utilization
Zhang, Yifei1; Zheng, Yunfei2; Hu, Jianwei1; Du, Ning1; Chen, Feng1
刊名PLOS ONE
2014-03-14
DOI10.1371/journal.pone.0091977
9期:3
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]SOURCE UTILIZATION PATTERNS ; BACTERIAL DIVERSITY ; SUBGINGIVAL PLAQUE ; ORAL-CAVITY ; PROFILES ; INDIVIDUALS ; BIOMASS ; SHIFTS ; SOILS
英文摘要

Chronic periodontitis is one of the most common forms of biofilm-induced diseases. Most of the recent studies were focus on the dental plaque microbial diversity and microbiomes. However, analyzing bacterial diversity at the taxonomic level alone limits deeper comprehension of the ecological relevance of the community. In this study, we compared the metabolic functional diversity of the microbial community in healthy subjects and periodontitis patients in a creative way-to assess the sole carbon source utilization using Biolog assay, which was first applied on oral micro-ecology assessment. Pattern analyses of 95-sole carbon sources catabolism provide a community-level phenotypic profile of the microbial community from different habitats. We found that the microbial community in the periodontitis group had greater metabolic activity compared to the microbial community in the healthy group. Differences in the metabolism of specific carbohydrates (e. g. beta-methyl-D-glucoside, stachyose, maltose, D-mannose, beta-methyl-D-glucoside and pyruvic acid) were observed between the healthy and periodontitis groups. Subjects from the healthy and periodontitis groups could be well distinguished by cluster and principle component analyses according to the utilization of discriminate carbon sources. Our results indicate significant difference in microbial functional diversity between healthy subjects and periodontitis patients. We also found Biolog technology is effective to further our understanding of community structure as a composite of functional abilities, and it enables the identification of ecologically relevant functional differences among oral microbial communities.

语种英语
WOS记录号WOS:000332858400128
项目编号81300880 ; 81200762 ; PKUSS20110301
资助机构National Natural Science Foundation of China ; Peking University School of Stomatology
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62147
专题北京大学口腔医学院
北京大学口腔医学院_科研处
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Sch Stomatol, Cent Lab, Beijing 100871, Peoples R China
2.Peking Univ, Sch Stomatol, Dept Periodontol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Yifei,Zheng, Yunfei,Hu, Jianwei,et al. Functional Diversity of the Microbial Community in Healthy Subjects and Periodontitis Patients Based on Sole Carbon Source Utilization[J]. PLOS ONE,2014,9(3).
APA Zhang, Yifei,Zheng, Yunfei,Hu, Jianwei,Du, Ning,&Chen, Feng.(2014).Functional Diversity of the Microbial Community in Healthy Subjects and Periodontitis Patients Based on Sole Carbon Source Utilization.PLOS ONE,9(3).
MLA Zhang, Yifei,et al."Functional Diversity of the Microbial Community in Healthy Subjects and Periodontitis Patients Based on Sole Carbon Source Utilization".PLOS ONE 9.3(2014).
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