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学科主题: 口腔医学
题名:
Functional Diversity of the Microbial Community in Healthy Subjects and Periodontitis Patients Based on Sole Carbon Source Utilization
作者: Zhang, Yifei1; Zheng, Yunfei2; Hu, Jianwei1; Du, Ning1; Chen, Feng1
刊名: PLOS ONE
发表日期: 2014-03-14
DOI: 10.1371/journal.pone.0091977
卷: 9, 期:3
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: SOURCE UTILIZATION PATTERNS ; BACTERIAL DIVERSITY ; SUBGINGIVAL PLAQUE ; ORAL-CAVITY ; PROFILES ; INDIVIDUALS ; BIOMASS ; SHIFTS ; SOILS
英文摘要:

Chronic periodontitis is one of the most common forms of biofilm-induced diseases. Most of the recent studies were focus on the dental plaque microbial diversity and microbiomes. However, analyzing bacterial diversity at the taxonomic level alone limits deeper comprehension of the ecological relevance of the community. In this study, we compared the metabolic functional diversity of the microbial community in healthy subjects and periodontitis patients in a creative way-to assess the sole carbon source utilization using Biolog assay, which was first applied on oral micro-ecology assessment. Pattern analyses of 95-sole carbon sources catabolism provide a community-level phenotypic profile of the microbial community from different habitats. We found that the microbial community in the periodontitis group had greater metabolic activity compared to the microbial community in the healthy group. Differences in the metabolism of specific carbohydrates (e. g. beta-methyl-D-glucoside, stachyose, maltose, D-mannose, beta-methyl-D-glucoside and pyruvic acid) were observed between the healthy and periodontitis groups. Subjects from the healthy and periodontitis groups could be well distinguished by cluster and principle component analyses according to the utilization of discriminate carbon sources. Our results indicate significant difference in microbial functional diversity between healthy subjects and periodontitis patients. We also found Biolog technology is effective to further our understanding of community structure as a composite of functional abilities, and it enables the identification of ecologically relevant functional differences among oral microbial communities.

语种: 英语
所属项目编号: 81300880 ; 81200762 ; PKUSS20110301
项目资助者: National Natural Science Foundation of China ; Peking University School of Stomatology
WOS记录号: WOS:000332858400128
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62147
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Stomatol, Cent Lab, Beijing 100871, Peoples R China
2.Peking Univ, Sch Stomatol, Dept Periodontol, Beijing 100871, Peoples R China

Recommended Citation:
Zhang, Yifei,Zheng, Yunfei,Hu, Jianwei,et al. Functional Diversity of the Microbial Community in Healthy Subjects and Periodontitis Patients Based on Sole Carbon Source Utilization[J]. PLOS ONE,2014,9(3).
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