IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Diagnostic model of saliva peptide finger print analysis of primary Sjogren′s syndrome patients by using weak cation exchange magnetic beads
Wei, Pan1; Kuo, Winston Patrick2; Chen, Feng3; Hua, Hong1
关键词mass spectrum peptidomics salivary diagnostics Sjogren&prime s syndrome (SS) weak cation exchange beads
刊名BIOSCIENCE REPORTS
2013
DOI10.1042/BSR20130022
33页:567-573
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]GEL-ELECTROPHORESIS ; MASS-SPECTROMETRY ; BIOMARKERS ; PROTEINS ; IDENTIFICATION ; PROTEOME ; CANCER
英文摘要

Saliva diagnostics has become an attractive field utilizing nanotechnology and molecular technologies for pSS (primary Sjogren′s syndrome). However, no specific methods have been established. To refine the diagnostic power of the saliva peptide finger print for the early detection of pSS, we screened the expression spectrum of salivary peptides in pSS patients by using mass spectrometry MALDI-TOF-MS (matrix-assisted laser-desorption ionization-time-of-flight MS) combined with magnetic bead. The present study was comprised 12 pSS patients and 13 healthy controls and broken down to two different phases. In the initial ′exploratory phase′, we enrolled seven pSS patients with eight age-and sex-matched healthy volunteers. Proteomics analysis of the unstimulated salivary samples was conducted to generate proportional peptide mass fingerprints. A diagnostic model was established. The testing cohort of the second ′validation phase′ was represented by five pSS patients and five age-and sex-matched healthy controls. The diagnostic power of this diagnostic panel was then validated. The results showed seven m/z (mass-to-charge) ratio peaks with significant differences. Five peptides were up-regulated and two down-regulated in the pSS patients compared with matched healthy subjects. In the validation phase, four out of five pSS patients were diagnosed as pSS, and four of the five healthy controls were diagnosed as healthy controls, respectively. Potential biomarkers were also primarily predicted. The novel diagnostic proteomic model with m/z peaks 1068.1 Da, 1196.2 Da, 1738.4 Da, 3375.3 Da, 3429.3 Da, 3449.7 Da and 3490.6 Da is of certain value for early diagnosis of pSS.

语种英语
WOS记录号WOS:000324032600003
项目编号30973318
资助机构National Natural Science Foundation of China
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62153
专题北京大学口腔医学院
北京大学口腔医学院_口腔黏膜科
作者单位1.Peking Univ, Sch Stomatol, Dept Oral Med & Tradit Chinese Med, Beijing 100081, Peoples R China
2.Harvard Univ, Sch Med, Harvard Catalyst Lab Innovat Translat Technol, Boston, MA USA
3.Peking Univ, Sch Stomatol, Cent Lab, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Wei, Pan,Kuo, Winston Patrick,Chen, Feng,et al. Diagnostic model of saliva peptide finger print analysis of primary Sjogren′s syndrome patients by using weak cation exchange magnetic beads[J]. BIOSCIENCE REPORTS,2013,33:567-573.
APA Wei, Pan,Kuo, Winston Patrick,Chen, Feng,&Hua, Hong.(2013).Diagnostic model of saliva peptide finger print analysis of primary Sjogren′s syndrome patients by using weak cation exchange magnetic beads.BIOSCIENCE REPORTS,33,567-573.
MLA Wei, Pan,et al."Diagnostic model of saliva peptide finger print analysis of primary Sjogren′s syndrome patients by using weak cation exchange magnetic beads".BIOSCIENCE REPORTS 33(2013):567-573.
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