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学科主题: 口腔医学
题名:
Diagnostic model of saliva peptide finger print analysis of primary Sjogren′s syndrome patients by using weak cation exchange magnetic beads
作者: Wei, Pan1; Kuo, Winston Patrick2; Chen, Feng3; Hua, Hong1
关键词: mass spectrum ; peptidomics ; salivary diagnostics ; Sjogren&prime ; s syndrome (SS) ; weak cation exchange beads
刊名: BIOSCIENCE REPORTS
发表日期: 2013
DOI: 10.1042/BSR20130022
卷: 33, 页:567-573
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: GEL-ELECTROPHORESIS ; MASS-SPECTROMETRY ; BIOMARKERS ; PROTEINS ; IDENTIFICATION ; PROTEOME ; CANCER
英文摘要:

Saliva diagnostics has become an attractive field utilizing nanotechnology and molecular technologies for pSS (primary Sjogren′s syndrome). However, no specific methods have been established. To refine the diagnostic power of the saliva peptide finger print for the early detection of pSS, we screened the expression spectrum of salivary peptides in pSS patients by using mass spectrometry MALDI-TOF-MS (matrix-assisted laser-desorption ionization-time-of-flight MS) combined with magnetic bead. The present study was comprised 12 pSS patients and 13 healthy controls and broken down to two different phases. In the initial ′exploratory phase′, we enrolled seven pSS patients with eight age-and sex-matched healthy volunteers. Proteomics analysis of the unstimulated salivary samples was conducted to generate proportional peptide mass fingerprints. A diagnostic model was established. The testing cohort of the second ′validation phase′ was represented by five pSS patients and five age-and sex-matched healthy controls. The diagnostic power of this diagnostic panel was then validated. The results showed seven m/z (mass-to-charge) ratio peaks with significant differences. Five peptides were up-regulated and two down-regulated in the pSS patients compared with matched healthy subjects. In the validation phase, four out of five pSS patients were diagnosed as pSS, and four of the five healthy controls were diagnosed as healthy controls, respectively. Potential biomarkers were also primarily predicted. The novel diagnostic proteomic model with m/z peaks 1068.1 Da, 1196.2 Da, 1738.4 Da, 3375.3 Da, 3429.3 Da, 3449.7 Da and 3490.6 Da is of certain value for early diagnosis of pSS.

语种: 英语
所属项目编号: 30973318
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000324032600003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62153
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Stomatol, Dept Oral Med & Tradit Chinese Med, Beijing 100081, Peoples R China
2.Harvard Univ, Sch Med, Harvard Catalyst Lab Innovat Translat Technol, Boston, MA USA
3.Peking Univ, Sch Stomatol, Cent Lab, Beijing 100081, Peoples R China

Recommended Citation:
Wei, Pan,Kuo, Winston Patrick,Chen, Feng,et al. Diagnostic model of saliva peptide finger print analysis of primary Sjogren′s syndrome patients by using weak cation exchange magnetic beads[J]. BIOSCIENCE REPORTS,2013,33:567-573.
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