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学科主题: 临床医学
题名:
Abnormal CD4+T helper (Th) 1 cells and activated memory B cells are associated with type III asymptomatic mixed cryoglobulinemia in HCV infection
作者: Kong, Fanyun1,2,3; Zhang, Wei1; Feng, Bo1; Zhang, Henghui1; Rao, Huiying1; Wang, Jianghua1; Cong, Xu1; Wei, Lai1
关键词: Hepatitis C ; Type III asymptomatic cryoglobulinemia ; CD4+T helper cells ; Activated memory B cells
刊名: VIROLOGY JOURNAL
发表日期: 2015-07-01
DOI: 10.1186/s12985-015-0324-2
卷: 12
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Virology
研究领域[WOS]: Virology
关键词[WOS]: CHRONIC HEPATITIS-C ; PERIPHERAL-BLOOD LYMPHOCYTES ; VIRUS-INFECTION ; SERUM-LEVELS ; AUTOIMMUNE-THYROIDITIS ; CIRCULATING CXCL11 ; INTERFERON-GAMMA ; HIGH VALUES ; CYTOKINES ; LIVER
英文摘要:

Background: Mixed cryoglobulinemia (MC) in hepatitis C virus (HCV) infection is associated with abnormal immune responses mediated by T cells and B cells, while the relationships of different subsets of CD4 + T helper (Th) cells, B cells and associated cytokines with type III asymptomatic MC in HCV infection are poorly understood.

Methods: Fifty-four chronic hepatitis C (CHC) patients and 23 healthy controls (HCs) were enrolled in the study. Serum cryoglobulins were detected by cryoprecipitation. The types of cryoglobulin were determined by western blot. The phenotypes and frequencies of Th cell and B cell subsets were detected by flow cytometric analysis. The cytokines IFN-gamma, IL-4, IL-17, IL-21, IL-22, and TGF-beta were measured by enzyme-linked immunosorbent assay.

Results: Twenty-six CHC patients were detected with type III asymptomatic MC. The frequencies of Th2, Th17, follicular helper T (Tfh cells), Th22, and tissue-like B cells were significantly higher in CHC patients compared to HCs, while these cell subsets were not significantly different between CHC patients and HCV-related MC patients. The frequencies of Th1 and activated memory B cells increased in HCV-related MC patients compared to HCs, although the difference between the two cell subsets in CHC patients and HCs was not significant. The frequency of regulatory T cells (Treg cells) was higher in CHC patients than in HCV-related MC patients and HCs. Higher expressions of serum IFN-gamma, IL-17, IL-21, and IL-22 were observed in CHC patients than in HCs, but the differences were not significantly different in CHC patients and HCV-related MC patients. The frequency of Th1 cells was associated with activated memory B cells in HCV-related MC patients, and the frequency of Th1 cells and activated memory B cells was closely related to HCV RNA in HCV-related MC patients.

Conclusions: The increased frequencies of Th17 cells, Tfh cells, Th22 cells, Treg cells, cytokines IL-17, IL-21, IL-22, and tissue-like B cells, were related to HCV infection but not type III asymptomatic MC. Higher frequencies of Th1 cells and activated memory B cells were associated with type III asymptomatic MC in HCV infection.

语种: 英语
所属项目编号: 2012ZX10002003
项目资助者: National Science and Technology Major Project for Infectious Diseases Control
WOS记录号: WOS:000357775200001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62197
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Beijing Key Lab Hepatitis & Immunotherapy Liver C, Inst Hepatol, Beijing 100044, Peoples R China
2.Xuzhou Med Coll, Dept Pathogen Biol, Xuzhou 221002, Jiangsu, Peoples R China
3.Xuzhou Med Coll, Lab Infect & Immunol, Xuzhou 221002, Jiangsu, Peoples R China

Recommended Citation:
Kong, Fanyun,Zhang, Wei,Feng, Bo,et al. Abnormal CD4+T helper (Th) 1 cells and activated memory B cells are associated with type III asymptomatic mixed cryoglobulinemia in HCV infection[J]. VIROLOGY JOURNAL,2015,12.
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