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学科主题临床医学
Nek9 regulates spindle organization and cell cycle progression during mouse oocyte meiosis and its location in early embryo mitosis
Yang, Shang-Wu1,2,3; Gao, Chen4; Chen, Lei1; Song, Ya-Li2; Zhu, Jin-Liang5; Qi, Shu-Tao1; Jiang, Zong-Zhe1; Wang, Zhong-Wei1; Lin, Fei1; Huang, Hao3; Xing, Fu-Qi2; Sun, Qing-Yuan1
关键词oocyte meiosis microtubule-organizing center (MTOC) spindle gamma-tubulin
刊名CELL CYCLE
2012-12-01
DOI10.4161/cc.22690
11期:23页:4366-4377
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
资助者National Basic Research Program of China ; National Natural Science Foundation of China ; Guang Dong Foshan city Technology project ; National Basic Research Program of China ; National Natural Science Foundation of China ; Guang Dong Foshan city Technology project
研究领域[WOS]Cell Biology
关键词[WOS]NIMA-FAMILY KINASE ; ACCURATE CHROMOSOME SEGREGATION ; POLO-LIKE KINASE-1 ; PROTEIN-KINASE ; GAMMA-TUBULIN ; MEIOTIC MATURATION ; MITOTIC SPINDLE ; MICROTUBULE NUCLEATION ; ASYMMETRIC DIVISION ; MAMMALIAN OOCYTES
英文摘要

Nek9 (also known as Nercc1), a member of the NIMA (never in mitosis A) family of protein kinases, regulates spindle formation, chromosome alignment and segregation in mitosis. Here, we showed that Nek9 protein was expressed from germinal vesicle (GV) to metaphase II (MII) stages in mouse oocytes with no detectable changes. Confocal microscopy identified that Nek9 was localized to the spindle poles at the metaphase stages and associated with the midbody at anaphase or telophase stage in both meiotic oocytes and the first mitotic embyros. Depletion of Nek9 by specific morpholino injection resulted in severely defective spindles and misaligned chromosomes with significant pro-MI/MI arrest and failure of first polar body (PB1) extrusion. Knockdown of Nek9 also impaired the spindle-pole localization of gamma-tubulin and resulted in retention of the spindle assembly checkpoint protein Bub3 at the kinetochores even after 10 h of culture. Live-cell imaging analysis also confirmed that knockdown of Nek9 resulted in oocyte arrest at the pro-MI/MI stage with abnormal spindles, misaligned chromosomes and failed polar body emission. Taken together, our results suggest that Nek9 may act as a MTOC-associated protein regulating microtubule nucleation, spindle organization and, thus, cell cycle progression during mouse oocyte meiotic maturation, fertilization and early embryo cleavage.

语种英语
所属项目编号2012CB944404 ; 2011CB944501 ; 30930065 ; 0005907120416038
资助者National Basic Research Program of China ; National Natural Science Foundation of China ; Guang Dong Foshan city Technology project ; National Basic Research Program of China ; National Natural Science Foundation of China ; Guang Dong Foshan city Technology project
WOS记录号WOS:000311951500020
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62216
专题北京大学第三临床医学院_生殖医学中心
作者单位1.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China
2.So Med Univ, Nanfang Hosp, Dept Ob Gy, Ctr Reprod Med, Guangzhou, Guangdong, Peoples R China
3.Nanhai Peoples Hosp, Dept Ob Gy, Foshan, Guangdong, Peoples R China
4.Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China
5.Peking Univ, Hosp 3, Dept Ob Gy, Ctr Reprod Med, Beijing 100871, Peoples R China
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GB/T 7714
Yang, Shang-Wu,Gao, Chen,Chen, Lei,et al. Nek9 regulates spindle organization and cell cycle progression during mouse oocyte meiosis and its location in early embryo mitosis[J]. CELL CYCLE,2012,11(23):4366-4377.
APA Yang, Shang-Wu.,Gao, Chen.,Chen, Lei.,Song, Ya-Li.,Zhu, Jin-Liang.,...&Sun, Qing-Yuan.(2012).Nek9 regulates spindle organization and cell cycle progression during mouse oocyte meiosis and its location in early embryo mitosis.CELL CYCLE,11(23),4366-4377.
MLA Yang, Shang-Wu,et al."Nek9 regulates spindle organization and cell cycle progression during mouse oocyte meiosis and its location in early embryo mitosis".CELL CYCLE 11.23(2012):4366-4377.
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