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学科主题: 基础医学
题名:
Local administration of TGF beta-1/VEGF(165) gene-transduced bone mesenchymal stem cells for Achilles allograft replacement of the anterior cruciate ligament in rabbits
作者: Wei, Xuelei1; Mao, Zebin2; Hou, Yu3; Lin, Lin3; Xue, Tao3; Chen, Lianxu3; Wang, Haijun3; Yu, Changlong3
关键词: TGF beta 1 ; VEGF(165) ; Gene therapy ; Adenovirus ; BMSC ; ACL
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2011-03-11
DOI: 10.1016/j.bbrc.2011.02.015
卷: 406, 期:2, 页:204-210
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: ENDOTHELIAL-GROWTH-FACTOR ; TRANSFORMING GROWTH-FACTOR-BETA-1 ; MATRIX SYNTHESIS ; STROMAL CELLS ; FIBROBLASTS ; RECONSTRUCTION ; AUTOGRAFT ; MODEL ; ACL
英文摘要:

Graft remodeling following anterior cruciate ligament (ACL) reconstruction requires a long period of recovery before it is capable of withstanding physiological loads. Graft revascularization is extremely important in the remodeling process. In ACL reconstruction, the local administration of vascular endothelial growth factor (VEGF) significantly increased revascularization of the graft, but did not significantly affect the mechanical properties of the graft after implantation (Ju et al., 2006; Yoshikawa, et al., 2006). Our previous studies showed that transforming growth factor-beta 1 (TGF beta 1) could promote improvements in mechanical strength in Achilles tendon regeneration, by regulating collagen type I and type III synthesis, cross-link formation, and matrix-remodeling (Hou et al., 2009). The current study aims to investigate whether the co-expression of TGF beta 1/VEGF(165) could beneficially affect the remodeling of ACL grafts. Bone marrow-derived mesenchymal stem cells (BMSCs), transfected with an adenoviral vector encoding TGF beta 1, VEGF(165) or TGF beta 1/VEGF(165), were surgically implanted into experimental ACL grafts, with non-transfected cells as a control. HE and toluidine blue staining, vascular number, and biomechanical features were analyzed at 3, 6, 12, and 24 weeks after surgery. The results suggest that TGF beta 1 expression, in the TGF beta 1/VEGF(165)-transfected BMSCs, could accelerate the remodeling of the reconstructed ligament. The cross-talk between TGF beta 1 and VEGF(165) has positive consequences, as TGF beta 1/VEGF(165)-transfected BMSCs significantly promoted angiogenesis of the reconstructed ligament at 3, 6, 12 weeks, with the best mechanical properties being achieved at 24 weeks. Furthermore, co-expression of these genes is more powerful and efficient than single gene therapy. (C) 2011 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 985-2-028-24 ; 09KZ66
项目资助者: State Sports General Administration of the People&prime ; s Republic of China ; Ministry of Education of the People&prime ; s Republic of China ; Tianjin Public Health Bureau
WOS记录号: WOS:000288616200009
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62229
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Tianjin Hosp, Dept Orthopaed Surg, Tianjin, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100871, Peoples R China
3.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100871, Peoples R China

Recommended Citation:
Wei, Xuelei,Mao, Zebin,Hou, Yu,et al. Local administration of TGF beta-1/VEGF(165) gene-transduced bone mesenchymal stem cells for Achilles allograft replacement of the anterior cruciate ligament in rabbits[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2011,406(2):204-210.
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