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学科主题: 基础医学
题名:
Hepatoprotective role of ganoderma lucidum polysaccharide against BCG-induced immune liver injury in mice
作者: Zhang, GL; Wang, YH; Ni, W; Teng, HL; Lin, ZB
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2002-08-01
卷: 8, 期:4, 页:728-733
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: NITRIC-OXIDE SYNTHASE ; CULTURED RAT HEPATOCYTES ; HEPATITIS-B ; HEPATOCELLULAR-CARCINOMA ; INTERFERON-ALPHA ; GENE-EXPRESSION ; PC12 CELLS ; KAPPA-B ; INHIBITION ; ENDOTOXIN
英文摘要:

AIM: To examine the effect of ganoderma lucidum polysaccharide (GLP) on the immune liver injury induced by BCG infection, and investigate the relationship between degrees of hepatic damage and NO production in mice.

METHODS: Immune hepatic injury was markedly induced by BCG-pretreatment (12S mg(.)kg(-1), 2-week, 1v) or by BCG-pretreatment plus lipopoiysaccharlde (LPS, 125 mug(.)kg(-1), 12-hour, iv) in mice in viva Hepatocellular damage induced by BCG-pretreated plus inflammatory cytokines mixture (CM), which was included TNF-alpha, IL-1beta, IFN-gamma and LPS in culture medium in vitro. Administration of GLP was performed by oral or incubating with culture medium at immune stimuli simultaneity. Liver damage was determined by activity of alanine aminotransferase (ALT) in serum and in hepatocytes cultured supernatant, by liver weight changes and histopathological examination. NO production in the cultured supernatant was determined by the Griess reaction. Moreover, inducible nitric oxide synthase (iNOS) protein expression was also examinated by immunohistochemical method.

RESULTS: Immune hepatic injury was markedly induced by BCG or BCG plus inflammatory cytokines in BALB/c mice in vivo and in vitro Under BCG-stimulated condition, augment of the liver weight and increase of the serum/ supernatant ALT level were observed, as well as granuloma forming and inflammatory cells soakage were observed by microscopic analysis within liver tissues. Moreover, NO production was also increased by BCG or/ and CM stimuli in the culture supernatant, and a lot of iNOS positive staining was observed in BCG-prestimulated hepatic sections. Application of GLP significantly mitigated hepatic tumefaction, decreased ALT enzyme release and NO production in serum/ supernatant, improved the pathological changes of chronic and acute inflammation induced by BCG-stimuli in mice. Moreover, the immunohistochemical result showed that GLP inhibited iNOS protein expression in BCG-immune hepatic damage model.

CONCLUSION: The present study indicates that NO participates in immune liver injury Induced by Mycobacterium bovis BCG infection. The mechanisms of protective roles by GLP for BCG-Induced immune liver injury may be due to influence NO production in mice.

语种: 英语
WOS记录号: WOS:000177562800029
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62234
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: Beijing Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, GL,Wang, YH,Ni, W,et al. Hepatoprotective role of ganoderma lucidum polysaccharide against BCG-induced immune liver injury in mice[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2002,8(4):728-733.
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