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学科主题: 药学
题名:
Enhanced Apoptotic Effects by the Combination of Curcumin and Methylseleninic Acid: Potential Role of Mcl-1 and FAK
作者: Guo, Xiao1; Yin, Shutao1; Dong, Yinhui1; Fan, Lihong2; Ye, Min3; Lu, Junxuan4; Hu, Hongbo1
关键词: curcumin ; methylseleninic acid ; apoptosis ; Mcl-1 ; FAK
刊名: MOLECULAR CARCINOGENESIS
发表日期: 2013-11-01
DOI: 10.1002/mc.21933
卷: 52, 期:11, 页:879-889
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Oncology
研究领域[WOS]: Biochemistry & Molecular Biology ; Oncology
关键词[WOS]: FOCAL ADHESION KINASE ; CANCER PREVENTION TRIAL ; HUMAN PROSTATE-CANCER ; BH3 MIMETIC ABT-737 ; SELENIUM SUPPLEMENTATION ; MOLECULAR TARGETS ; INDUCE APOPTOSIS ; HUMAN PC-3 ; CELLS ; CARCINOMA
英文摘要:

Curcumin and methylseleninic acid (MSeA) are well-documented dietary chemopreventive agents. Apoptosis appears to be a major mechanism for both agents to exert anti-cancer activity. The purpose of the present study was designed to determine whether the apoptotic effect on human cancer cells can be enhanced by combining curcumin with MSeA. Apoptosis was evaluated by Annexin V staining of externalized phosphatidylserine by flow cytometry. Expression of protein was analyzed by Western blotting. Localization of apoptosis-inducing factor (AIF) was detected by immunocytochemistry. RNA interference was employed to inhibit expression of specific protein. We found here that combining curcumin with MSeA led to a significantly enhanced apoptosis in both MDA-MB-231 breast cancer cells and DU145 prostate cancer cells. Further mechanistic investigations revealed that curcumin treatment alone caused a concentration dependent upregulation of Mcl-1, which can be overcome by combining it with MSeA. In line with the Mcl-1 reduction, an enhanced mitochondrial permeability transition and AIF nuclear translocation by the combination were achieved. In addition, an increased suppression of focal adhesion kinase activity was observed in the combination-treated cells which were associated with cell detachment-induced apoptosis by the combination. Our findings suggest that curcumin/MSeA combination holds excellent potential for improving their efficacy against human breast and prostate cancer through enhanced apoptosis induction. (c) 2012 Wiley Periodicals, Inc.

语种: 英语
所属项目编号: 31071533 ; 30972172 ; 2009-2-11
项目资助者: National Natural Science Foundation of China (NSFC) ; Chinese Universities Scientific Fund
WOS记录号: WOS:000328469900003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62241
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.China Agr Univ, Div Nutr & Hlth, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
2.China Agr Univ, Coll Vet Med, Beijing 100083, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Hlth Sci Ctr, Beijing 100871, Peoples R China
4.Texas Tech Univ, Sch Pharm, Dept Biomed Sci, Amarillo, TX USA

Recommended Citation:
Guo, Xiao,Yin, Shutao,Dong, Yinhui,et al. Enhanced Apoptotic Effects by the Combination of Curcumin and Methylseleninic Acid: Potential Role of Mcl-1 and FAK[J]. MOLECULAR CARCINOGENESIS,2013,52(11):879-889.
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