IR@PKUHSC  > 北京大学第一临床医学院  > 核医学科
学科主题临床医学
SOX9 is a regulator of ADAMTSs-induced cartilage degeneration at the early stage of human osteoarthritis
Zhang, Q.1,5; Ji, Q.1; Wang, X.3; Kang, L.4; Fu, Y.1; Yin, Y.1; Li, Z.1; Liu, Y.1; Xu, X.2; Wang, Y.1
关键词Osteoarthritis Cartilage Chondrocyte ADAMTS SOX9
刊名OSTEOARTHRITIS AND CARTILAGE
2015-12-01
DOI10.1016/j.joca.2015.06.014
23期:12页:2259-2268
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Orthopedics ; Rheumatology
研究领域[WOS]Orthopedics ; Rheumatology
关键词[WOS]OLIGOMERIC MATRIX PROTEIN ; CHONDROCYTE DIFFERENTIATION ; CELL-PROLIFERATION ; DEGRADATION ; METALLOPROTEINASES ; ACTIVATION ; MECHANISMS ; DISEASE ; CANCER ; HPIP
英文摘要

Objective: To identify whether cartilage master regulator SRY-related protein 9 (SOX9) mediates A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) dysregulation during osteoarthritis (OA) cartilage degeneration.

Method: Twenty-two randomly selected OA patients were evaluated using Outerbridge Classification via arthroscopy. Haematoxyline-eosin (HE), Safranin O and Masson staining were performed for the histopathological assessment. The expression of ADAMTSs, collagen 2A1 (COL2A1), aggrecan (ACAN), cartilage oligomeric matrix protein (COMP) and SOX9 were examined using real-time quantitative Polymerase Chain Reaction (PCR) (RT-qPCR) and western blotting analysis. Immunohistochemistry (IHC) analysis was performed to investigate the production of ADAMTSs in cartilage tissues. The association between SOX9 production and ADAMTSs, COL2A1, ACAN, and COMP expression was established by full-depth cartilage biopsies.

Results: ADAMTSs expression levels were repressed at stage 1, while a significant increase was observed at the progressive stage of OA. SOX9 was upregulated at stage 1 and suppressed at a later stage of cartilage development, particularly in cartilage with severe damage. In addition, SOX9 repressed the expression of ADAMTSs and promoted COL2A1, ACAN and COMP expression in human chondrocytes. SOX9 was recruited to the promoters of ADAMTS-4 and ADAMTS-7. SOX9 expression was negatively correlated with ADAMTSs production and was positively associated with COL2A1, ACAN and COMP expression. Inhibition of ADAMTSs markedly increased the production of COL2A1, ACAN and COMP in chondrocytes isolated from the early stage of OA.

Conclusions: These findings indicated that SOX9 upregulation might mediate ADAMTSs suppression at the early stage of human OA. In addition, SOX9 could be used as a potential therapeutic agent for human OA at an early stage. (C) 2015 The Authors. Published by Elsevier Ltd and Osteoarthritis Research Society International.

语种英语
WOS记录号WOS:000366838900023
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62246
Collection北京大学第一临床医学院_核医学科
北京大学精神卫生研究所_精神科
作者单位1.Gen Hosp Chinese Peoples Liberat Army, Dept Orthopaed, Beijing 100853, Peoples R China
2.Beijing Inst Biotechnol, Beijing 100850, Peoples R China
3.Gen Hosp Chinese Peoples Liberat Army, Dept Neurol, Beijing 100853, Peoples R China
4.Peking Univ First Hosp, Dept Nucl Med, Beijing 100034, Peoples R China
5.Royal Liverpool Univ Hosp, Dept Orthopaed Surg, Liverpool, Merseyside, England
Recommended Citation
GB/T 7714
Zhang, Q.,Ji, Q.,Wang, X.,et al. SOX9 is a regulator of ADAMTSs-induced cartilage degeneration at the early stage of human osteoarthritis[J]. OSTEOARTHRITIS AND CARTILAGE,2015,23(12):2259-2268.
APA Zhang, Q..,Ji, Q..,Wang, X..,Kang, L..,Fu, Y..,...&Wang, Y..(2015).SOX9 is a regulator of ADAMTSs-induced cartilage degeneration at the early stage of human osteoarthritis.OSTEOARTHRITIS AND CARTILAGE,23(12),2259-2268.
MLA Zhang, Q.,et al."SOX9 is a regulator of ADAMTSs-induced cartilage degeneration at the early stage of human osteoarthritis".OSTEOARTHRITIS AND CARTILAGE 23.12(2015):2259-2268.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Zhang, Q.]'s Articles
[Ji, Q.]'s Articles
[Wang, X.]'s Articles
百度学术
百度学术Similar articles in
[Zhang, Q.]'s Articles
[Ji, Q.]'s Articles
[Wang, X.]'s Articles
必应学术
必应学术Similar articles in
[Zhang, Q.]'s Articles
[Ji, Q.]'s Articles
[Wang, X.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.