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An enzymatic cycling method for the determination of serum total bile acids with recombinant 3 alpha-hydroxysteroid dehydrogenase
Zhang, GH; Cong, AR; Xu, GB; Li, CB; Yang, RF; Xia, TA
关键词bile acids enzymatic cycling method 3 alpha-hydroxysteroid dehydrogenase
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2005-01-07
DOI10.1016/j.bbrc.2004.11.005
326期:1页:87-92
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]3ALPHA-HYDROXYSTEROID DEHYDROGENASE ; PSEUDOMONAS-TESTOSTERONI ; PURIFICATION ; CHOLESTASIS ; URINE
英文摘要

A highly sensitive enzymatic cycling method was developed for the serum total bile acids assay. We constructed a prokaryotic expression system to prepare the recombinant 3alpha-hydroxysteroid dehydrogenase in place of the natural enzyme and for the first time used it in the total bile acids assay. The production rate of thio-NADH correlated with the bile acids concentration and was measured by the change of absorbance at 405/660 nm. The enzymatic cycling method could detect 0.22 mumol/L total bile acids in serum. Within-run and between-run imprecisions were 1.2-3.7% and 2.3-4.8%, respectively. The calibration curve for total bile acids in serum was linear between 0.5 and 180 mumol/L. This method was free from interference by bilirubin, hemoglobin, ascorbate, and lactate dehydrogenase. In conclusion, serum total bile acids could be measured by the enzymatic cycling method with recombinant 3alpha-hydroxysteroid dehydrogenase as the tool enzyme. (C) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000225776600013
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62262
专题北京大学第一临床医学院
作者单位1.Peking Univ, First Hosp, Dept Clin Lab, Beijing, Peoples R China
2.Shandong Weihai Wendeng Cent Hosp, Weihai, Peoples R China
推荐引用方式
GB/T 7714
Zhang, GH,Cong, AR,Xu, GB,et al. An enzymatic cycling method for the determination of serum total bile acids with recombinant 3 alpha-hydroxysteroid dehydrogenase[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,326(1):87-92.
APA Zhang, GH,Cong, AR,Xu, GB,Li, CB,Yang, RF,&Xia, TA.(2005).An enzymatic cycling method for the determination of serum total bile acids with recombinant 3 alpha-hydroxysteroid dehydrogenase.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,326(1),87-92.
MLA Zhang, GH,et al."An enzymatic cycling method for the determination of serum total bile acids with recombinant 3 alpha-hydroxysteroid dehydrogenase".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 326.1(2005):87-92.
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