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学科主题: 口腔医学
题名:
Cytotoxicity of Silver Nanoparticles in Human Embryonic Stem Cell-Derived Fibroblasts and an L-929 Cell Line
作者: Peng, Hui1,2; Zhang, Xuehui1,2; Wei, Yan1,2; Liu, Wentao1,2; Li, Shenglin3,4,5,6; Yu, Guangyan7,8; Fu, Xin9; Cao, Tong9; Deng, Xuliang1,2
刊名: JOURNAL OF NANOMATERIALS
发表日期: 2012
DOI: 10.1155/2012/160145
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
研究领域[WOS]: Science & Technology - Other Topics ; Materials Science
关键词[WOS]: DENDRITIC CELLS ; QUANTUM-DOT ; EMBRYOTOXICITY ; GENOTOXICITY ; MECHANISMS ; TOXICITY ; RATS ; SIZE
英文摘要:

Consensus about the toxicity of silver nanoparticles (Ag-NPs) has not been reached, even though extensive attention has been paid to this issue. This confusion may be due to physicochemical factors of Ag-NPs and the cell model used for biological safety evaluation. In the present study, human embryonic stem cell-derived fibroblasts (EBFs), which have been considered a closer representative of the in vivo response, were used as a novel cell model to assess the cytotoxicity of Ag-NPs (similar to 20 nm and similar to 100 nm) in comparison with L-929 fibroblast cell line. Cell proliferation, cell cycle, apoptosis, p53 expression, and cellular uptake were examined. Results showed that Ag-NPs presented higher cytotoxicity to EBF than to L-929. EBF demonstrated a stronger capacity to ingest Ag-NPs, a higher G2/M arrest, and more upgraduated p53 expression after exposed to Ag-NPs for 48 h when compared with L-929. It could be concluded that EBF exhibited a more sensitive response to Ag-NPs compared with L-929 cells, indicating that EBF may be a valid candidate for cytotoxicity screening assays of nanoparticles.

语种: 英语
所属项目编号: 2012CB933904 ; 81171000 ; 2011DFA32190
项目资助者: National Basic Research Program of China ; National Natural Science Foundation of China ; International S&amp ; T Cooperation Program of China (ISTCP)
WOS记录号: WOS:000307658700001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62269
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Dept Geriatr Dent, Sch Stomatol, Beijing 100081, Peoples R China
2.Peking Univ, Dept Geriatr Dent, Hosp Stomatol, Beijing 100081, Peoples R China
3.Peking Univ, Cent Lab, Sch Stomatol, Beijing 100081, Peoples R China
4.Peking Univ, Cent Lab, Hosp Stomatol, Beijing 100081, Peoples R China
5.Peking Univ, Lab Oral & Maxillofacial Surg, Sch Stomatol, Beijing 100081, Peoples R China
6.Peking Univ, Lab Oral & Maxillofacial Surg, Hosp Stomatol, Beijing 100081, Peoples R China
7.Peking Univ, Dept Oral & Maxillofacial Surg, Sch Stomatol, Beijing 100081, Peoples R China
8.Peking Univ, Dept Oral & Maxillofacial Surg, Hosp Stomatol, Beijing 100081, Peoples R China
9.Natl Univ Singapore, Fac Dent Res Labs, Singapore 119083, Singapore

Recommended Citation:
Peng, Hui,Zhang, Xuehui,Wei, Yan,et al. Cytotoxicity of Silver Nanoparticles in Human Embryonic Stem Cell-Derived Fibroblasts and an L-929 Cell Line[J]. JOURNAL OF NANOMATERIALS,2012.
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