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学科主题: 临床医学
题名:
Molecular interactions of re-released proteins in electrophoresis of human erythrocytes
作者: Su, Yan; Shen, Jing2; Gao, Lijun; Tian, Huifang2; Tian, Zhihua2; Qin, Wenbin1
关键词: Carbonic anhydrase ; Erythrocyte ; Hemoglobin ; Interaction
刊名: ELECTROPHORESIS
发表日期: 2012-05-01
DOI: 10.1002/elps.201100644
卷: 33, 期:9-10, 页:1402-1405
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ; CELL ELECTROPHORESIS ; CARBONIC-ANHYDRASE ; FLUORESCENCE DETECTION ; SINGLE ERYTHROCYTES ; BLOOD-CELLS ; HEMOGLOBIN ; SEPARATION ; BAND-3
英文摘要:

Recently, we found that hemoglobin (Hb) could be re-released from live erythrocytes during electrophoresis release test (ERT). The re-released Hb displays single-band and multiple-band re-release types, but its exact mechanism is not well understood. In this article, the protein components of the single-band re-released Hb were examined. First, the re-released band of erythrocytes and the corresponding band of hemolysate, which was used as control, were cut out from starch-agarose mixed gel. Next, proteins were recovered from the starch-agarose mixed gel by freeze-thaw method. After condensing in a vacuum freeze drier, the samples were loaded onto a 512% SDS-PAGE. After electrophoresis, three protein bands (16, 28.9, and 29.3 kDa) emerged from the erythrocytes re-released Hb single-band (R-R), but only one band (29.3 kDa) emerged from the corresponding hemolysate control band (H-R). Finally, these bands were analyzed by MALDITOF MS. The results showed that these proteins were beta-globin (16 kDa), carbonic anhydrase 1 (CA1, 28.9 kDa), and carbonic anhydrase 2 (CA2, 29.3 kDa). Because CA2 exists in both erythrocytes re-released band and hemolysate control band, we conclude that the single-band re-released Hb is mainly composed of HbA and CA1. Studying the possible interaction between HbA and CA1 will help us further understand the in vivo function of Hb.

语种: 英语
所属项目编号: NJ09157 ; 2010BS1101 ; 81160214
项目资助者: Major Projects of Higher Education Scientific Research in the Inner Mongolia Autonomous Region ; Ministry of Education ; Natural Science Foundation of Inner Mongolia ; Natural Science Foundation of China
WOS记录号: WOS:000304600500008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62277
Appears in Collections:北京大学临床肿瘤学院_中心实验室_期刊论文

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作者单位: 1.Baotou Med Coll, Lab Hemoglobin, Dept Biochem & Mol Biol, Baotou 014060, Inner Mongolia, Peoples R China
2.Peking Univ, Canc Hosp & Inst, Cent Lab, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China

Recommended Citation:
Su, Yan,Shen, Jing,Gao, Lijun,et al. Molecular interactions of re-released proteins in electrophoresis of human erythrocytes[J]. ELECTROPHORESIS,2012,33(9-10):1402-1405.
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