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学科主题临床医学
Molecular interactions of re-released proteins in electrophoresis of human erythrocytes
Su, Yan; Shen, Jing2; Gao, Lijun; Tian, Huifang2; Tian, Zhihua2; Qin, Wenbin1
关键词Carbonic anhydrase Erythrocyte Hemoglobin Interaction
刊名ELECTROPHORESIS
2012-05-01
DOI10.1002/elps.201100644
33期:9-10页:1402-1405
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]CAPILLARY-ELECTROPHORESIS ; CELL ELECTROPHORESIS ; CARBONIC-ANHYDRASE ; FLUORESCENCE DETECTION ; SINGLE ERYTHROCYTES ; BLOOD-CELLS ; HEMOGLOBIN ; SEPARATION ; BAND-3
英文摘要

Recently, we found that hemoglobin (Hb) could be re-released from live erythrocytes during electrophoresis release test (ERT). The re-released Hb displays single-band and multiple-band re-release types, but its exact mechanism is not well understood. In this article, the protein components of the single-band re-released Hb were examined. First, the re-released band of erythrocytes and the corresponding band of hemolysate, which was used as control, were cut out from starch-agarose mixed gel. Next, proteins were recovered from the starch-agarose mixed gel by freeze-thaw method. After condensing in a vacuum freeze drier, the samples were loaded onto a 512% SDS-PAGE. After electrophoresis, three protein bands (16, 28.9, and 29.3 kDa) emerged from the erythrocytes re-released Hb single-band (R-R), but only one band (29.3 kDa) emerged from the corresponding hemolysate control band (H-R). Finally, these bands were analyzed by MALDITOF MS. The results showed that these proteins were beta-globin (16 kDa), carbonic anhydrase 1 (CA1, 28.9 kDa), and carbonic anhydrase 2 (CA2, 29.3 kDa). Because CA2 exists in both erythrocytes re-released band and hemolysate control band, we conclude that the single-band re-released Hb is mainly composed of HbA and CA1. Studying the possible interaction between HbA and CA1 will help us further understand the in vivo function of Hb.

语种英语
WOS记录号WOS:000304600500008
项目编号NJ09157 ; 2010BS1101 ; 81160214
资助机构Major Projects of Higher Education Scientific Research in the Inner Mongolia Autonomous Region ; Ministry of Education ; Natural Science Foundation of Inner Mongolia ; Natural Science Foundation of China
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62277
专题北京大学临床肿瘤学院_中心实验室
作者单位1.Baotou Med Coll, Lab Hemoglobin, Dept Biochem & Mol Biol, Baotou 014060, Inner Mongolia, Peoples R China
2.Peking Univ, Canc Hosp & Inst, Cent Lab, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Su, Yan,Shen, Jing,Gao, Lijun,et al. Molecular interactions of re-released proteins in electrophoresis of human erythrocytes[J]. ELECTROPHORESIS,2012,33(9-10):1402-1405.
APA Su, Yan,Shen, Jing,Gao, Lijun,Tian, Huifang,Tian, Zhihua,&Qin, Wenbin.(2012).Molecular interactions of re-released proteins in electrophoresis of human erythrocytes.ELECTROPHORESIS,33(9-10),1402-1405.
MLA Su, Yan,et al."Molecular interactions of re-released proteins in electrophoresis of human erythrocytes".ELECTROPHORESIS 33.9-10(2012):1402-1405.
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