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学科主题: 临床医学
题名:
Submacular DL-alpha-Aminoadipic Acid Eradicates Primate Photoreceptors but Does Not Affect Luteal Pigment or the Retinal Vasculature
作者: Shen, Weiyong1; Zhang, Jun2; Chung, Sook Hyun1; Hu, Yuntao2; Ma, Zhizhong2; Gillies, Mark C.1
刊名: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
发表日期: 2011
DOI: 10.1167/iovs.10-6033
卷: 52, 期:1, 页:119-127
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: IDIOPATHIC MACULAR TELANGIECTASIA ; OPTICAL COHERENCE TOMOGRAPHY ; CYSTINE-GLUTAMATE ANTIPORTER ; MULLER GLIAL-CELL ; PERIFOVEAL TELANGIECTASIA ; INTRAVITREAL BEVACIZUMAB ; BINDING PROTEIN ; FOVEA CENTRALIS ; TRANSGENIC MICE ; IN-VIVO
英文摘要:

PURPOSE. Macular telangiectasia type 2 (MT2) is a condition of uncertain etiology characterized by retinal vascular abnormalities, depletion of luteal pigment, and photoreceptor loss. To model this condition, the authors recently used a purportedly glial-selective toxin, DL-alpha-aminoadipic acid (DL-alpha-AAA), to test the effect of Muller cell disruption on the blood-retinal barrier in rats. In this study, they investigated macular changes after subretinal injection of DL-alpha-AAA in monkeys.

METHODS. Various doses of DL-alpha-AAA were injected beneath the macula in eight monkey eyes. Eyes were examined by multifocal electroretinography (mfERG), optical coherence tomography (OCT), fundus autofluorescence, color photography, and fluorescein angiography. Five months after injection, eyes were examined by histology and immunohistochemistry for changes in photoreceptors and the retinal glia. In vitro studies evaluated the effect of DL-alpha-AAA on 661W cone photoreceptor viability.

RESULTS. Subretinal injection of DL-alpha-AAA resulted in virtually complete ablation of photoreceptors in the injected area, as shown by OCT and histology, and severely impaired mfERG responses. Muller cells, albeit activated, survived the injury. Macular pigment remained unchanged in the central fovea. Subretinal injection of DL-alpha-AAA did not induce vascular leakage, though it increased the fundus autofluorescence. DL-alpha-AAA had a dose-dependent toxic effect on 661W photoreceptors.

CONCLUSIONS. Submacular injection of DL-alpha-AAA induced severe damage to photoreceptors but failed to eliminate Muller cells in monkeys. Central macular pigment persisted despite loss of photoreceptors, and the retinal vasculature was unaffected. These observations may have significance in studying the roles of different cellular components in the pathogenesis of MT2. (Invest Ophthalmol Vis Sci. 2011; 52: 119-127) DOI: 10.1167/iovs.10-6033

语种: 英语
项目资助者: Lowy Medical Research Institute, Australia ; University of Sydney
WOS记录号: WOS:000285925000017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62286
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Ctr Eye, Hosp 3, Beijing 100871, Peoples R China
2.Univ Sydney, Retinal Therapeut Res Grp, Save Sight Inst, Sydney, NSW 2000, Australia

Recommended Citation:
Shen, Weiyong,Zhang, Jun,Chung, Sook Hyun,et al. Submacular DL-alpha-Aminoadipic Acid Eradicates Primate Photoreceptors but Does Not Affect Luteal Pigment or the Retinal Vasculature[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2011,52(1):119-127.
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