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学科主题: 临床医学
题名:
Pegylated interferon alpha enhances recovery of memory T cells in e antigen positive chronic hepatitis B patients
作者: Liu, Yong Zhe1,2; Hou, Feng Qin1,2; Ding, Peng3; Ren, Yuan Yuan1,2; Li, Shi Hong1,2; Wang, Gui Qiang1,2
关键词: Chronic hepatitis B ; Pegylated interferon-alpha therapy ; Memory T cell ; Intracytoplasmic cytokine staining (ICCS)
刊名: VIROLOGY JOURNAL
发表日期: 2012-11-16
DOI: 10.1186/1743-422X-9-274
卷: 9
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Virology
研究领域[WOS]: Virology
关键词[WOS]: CHRONIC VIRAL-INFECTION ; VIRUS-INFECTION ; IMMUNE-RESPONSE ; CD127 EXPRESSION ; TRANSGENIC MICE ; HBV INFECTION ; C-VIRUS ; INDUCTION ; DIFFERENTIATION ; REPLICATION
英文摘要:

Background: Interferons (IFNs) are a group of cytokines commonly used in the clinical treatment of chronic hepatitis B (CHB) patients. Their therapeutic effects are highly correlated with recovery of host antiviral immunity. Clearance of hepatitis B virus (HBV) is mediated partially by activated functional memory T cells. The aims of the present study were to investigate memory T cell status in patients with different outcomes following pegylated interferon-alpha (IFN-alpha) therapy and to identify new biomarkers for predicting antiviral immune responses.

Methods: Peripheral blood cells were isolated from 23 CHB patients who were treated with pegylated IFN-alpha at week 0 (baseline) and week 24. Co-expression of programmed death-1 (PD-1) and CD244 in CD45RO positive T cells, as well as a subset of CD127 and CXCR4 positive memory T cells were assessed. In addition, perforin, granzyme B, and interferon-gamma (IFN-gamma) expressions were also analyzed by flow cytometric analysis after intracytoplasmic cytokine staining (ICCS). Peripheral blood mononuclear cells (PBMC) isolated at week 24 were re-challenged with exogenous HBV core antigen, and the percentage of IFN-gamma expression, serum HBV DNA loads, and ALT (alanine aminotransferase) levels were evaluated.

Results: At week 24, PD-1 and CD244 expression in CD8 memory T cells were down-regulated (P < 0.05, P < 0.05, respectively), along with decreased HBV DNA loads (P < 0.05), while the expressions of partial effector molecules in CD8 and CD4 memory T cells was up-regulated (P < 0.05, P < 0.05, respectively), especially in the responders. CD127 and CXCR4 were highly expressed in CD8 memory T cells after pegylated IFN-alpha treatment (P < 0.05), which was inversely correlated with HBV DNA loads (r = -0.47, P = 0.001). The responders had a higher IFN-gamma expression in memory T cells than the non-responders did after HBV antigen re-stimulation in vitro.

Conclusion: Pegylated IFN-alpha treatment enhanced recovery of memory T cells in CHB patients by down-regulating inhibitory receptors and up-regulating effector molecules. The expressions of CXCR4 and CD127 in CD8 memory T cell may be used as biomarkers for predicting the outcome of treatment.

语种: 英语
所属项目编号: 30771905 ; CB512800 ; 008ZX10002-008 ; D08050700650803
项目资助者: National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Mega-projects of Science Research ; Beijing Municipal Science &amp ; Technology Commission
WOS记录号: WOS:000312265100001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62302
Appears in Collections:北京大学第一临床医学院_感染疾病科_期刊论文

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作者单位: 1.Peking Univ, Dept Infect Dis, Hosp 1, Beijing 100034, Peoples R China
2.Peking Univ, Res Ctr Liver Dis, Hosp 1, Beijing 100034, Peoples R China
3.Hebei United Univ, Coll Publ Hlth, Tangshan 063000, Hebei Province, Peoples R China

Recommended Citation:
Liu, Yong Zhe,Hou, Feng Qin,Ding, Peng,et al. Pegylated interferon alpha enhances recovery of memory T cells in e antigen positive chronic hepatitis B patients[J]. VIROLOGY JOURNAL,2012,9.
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