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Pegylated interferon alpha enhances recovery of memory T cells in e antigen positive chronic hepatitis B patients
Liu, Yong Zhe1,2; Hou, Feng Qin1,2; Ding, Peng3; Ren, Yuan Yuan1,2; Li, Shi Hong1,2; Wang, Gui Qiang1,2
关键词Chronic hepatitis B Pegylated interferon-alpha therapy Memory T cell Intracytoplasmic cytokine staining (ICCS)
刊名VIROLOGY JOURNAL
2012-11-16
DOI10.1186/1743-422X-9-274
9
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Virology
研究领域[WOS]Virology
关键词[WOS]CHRONIC VIRAL-INFECTION ; VIRUS-INFECTION ; IMMUNE-RESPONSE ; CD127 EXPRESSION ; TRANSGENIC MICE ; HBV INFECTION ; C-VIRUS ; INDUCTION ; DIFFERENTIATION ; REPLICATION
英文摘要

Background: Interferons (IFNs) are a group of cytokines commonly used in the clinical treatment of chronic hepatitis B (CHB) patients. Their therapeutic effects are highly correlated with recovery of host antiviral immunity. Clearance of hepatitis B virus (HBV) is mediated partially by activated functional memory T cells. The aims of the present study were to investigate memory T cell status in patients with different outcomes following pegylated interferon-alpha (IFN-alpha) therapy and to identify new biomarkers for predicting antiviral immune responses.

Methods: Peripheral blood cells were isolated from 23 CHB patients who were treated with pegylated IFN-alpha at week 0 (baseline) and week 24. Co-expression of programmed death-1 (PD-1) and CD244 in CD45RO positive T cells, as well as a subset of CD127 and CXCR4 positive memory T cells were assessed. In addition, perforin, granzyme B, and interferon-gamma (IFN-gamma) expressions were also analyzed by flow cytometric analysis after intracytoplasmic cytokine staining (ICCS). Peripheral blood mononuclear cells (PBMC) isolated at week 24 were re-challenged with exogenous HBV core antigen, and the percentage of IFN-gamma expression, serum HBV DNA loads, and ALT (alanine aminotransferase) levels were evaluated.

Results: At week 24, PD-1 and CD244 expression in CD8 memory T cells were down-regulated (P < 0.05, P < 0.05, respectively), along with decreased HBV DNA loads (P < 0.05), while the expressions of partial effector molecules in CD8 and CD4 memory T cells was up-regulated (P < 0.05, P < 0.05, respectively), especially in the responders. CD127 and CXCR4 were highly expressed in CD8 memory T cells after pegylated IFN-alpha treatment (P < 0.05), which was inversely correlated with HBV DNA loads (r = -0.47, P = 0.001). The responders had a higher IFN-gamma expression in memory T cells than the non-responders did after HBV antigen re-stimulation in vitro.

Conclusion: Pegylated IFN-alpha treatment enhanced recovery of memory T cells in CHB patients by down-regulating inhibitory receptors and up-regulating effector molecules. The expressions of CXCR4 and CD127 in CD8 memory T cell may be used as biomarkers for predicting the outcome of treatment.

语种英语
WOS记录号WOS:000312265100001
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被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62302
专题北京大学第一临床医学院_感染疾病科
作者单位1.Peking Univ, Dept Infect Dis, Hosp 1, Beijing 100034, Peoples R China
2.Peking Univ, Res Ctr Liver Dis, Hosp 1, Beijing 100034, Peoples R China
3.Hebei United Univ, Coll Publ Hlth, Tangshan 063000, Hebei Province, Peoples R China
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GB/T 7714
Liu, Yong Zhe,Hou, Feng Qin,Ding, Peng,et al. Pegylated interferon alpha enhances recovery of memory T cells in e antigen positive chronic hepatitis B patients[J]. VIROLOGY JOURNAL,2012,9.
APA Liu, Yong Zhe,Hou, Feng Qin,Ding, Peng,Ren, Yuan Yuan,Li, Shi Hong,&Wang, Gui Qiang.(2012).Pegylated interferon alpha enhances recovery of memory T cells in e antigen positive chronic hepatitis B patients.VIROLOGY JOURNAL,9.
MLA Liu, Yong Zhe,et al."Pegylated interferon alpha enhances recovery of memory T cells in e antigen positive chronic hepatitis B patients".VIROLOGY JOURNAL 9(2012).
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