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IR@PKUHSC  > 北京大学第三临床医学院  > 神经内科  > 期刊论文
学科主题: 临床医学
题名:
Protective Effect of Sinomenine on Cartilage Degradation and Chondrocytes Apoptosis
作者: Ju, Xiao-dong2; Deng, Min1; Ao, Ying-fang2; Yu, Chang-long2; Wang, Jian-quan2; Yu, Jia-kuo2; Cui, Guo-qing2; Hu, Yue-lin2
关键词: sinomenine ; cartilage ; chondrocyte ; apoptosis ; matrix metalloproteinase-13 ; tissue inhibitor of metalloproteinase-1
刊名: YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN
发表日期: 2010-08-01
卷: 130, 期:8, 页:1053-1060
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacolo !E tr>
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: GENE-EXPRESSION ; MATRIX METALLOPROTEINASES ; THERAPEUTIC TARGETS ; IN-VITRO ; OSTEOARTHRITIS ; SYNOVIOCYTES ; ARTHRITIS ; INTERLEUKIN-1 ; COLLAGENASE ; INHIBITION
英文摘要:

Sinomenine (SIN), an alkaloid extracted from the stem of the Chinese medicinal plant sinomenium acutum, has been used for treating rheumatoid arthritis. But little is known whether SIN has a protective effect on osteoarthritis (OA). In this study, we investigated the protective effect of SIN on IL-1 beta-induced proteoglycan degradation and apoptosis in rabbit articular cartilage and chondrocytes. Treatment with 10 ng/ml IL-1 beta increased the level of glycosaminoglycan (GAG) released into the culture media, and up-regulated the activity and mRNA expression of matrix metalloproteinase 13 (MMP-13) and down-regulated the activity and mRNA expression of tissue inhibitor of metalloproteinase 1 (TIMP-1) in cartilage explants, as confirmed by the methods of GAG quantitation, MMP-13/TIMP-1 enzyme-linked immunosorbent assay (ELISA) and real-time quantitative RT-PCR. Treatment with 10 ng/ml IL-1 beta resulted in marked apoptosis in chondrocytes, as demonstrated by decreased cell viability, occurrence of DNA laddering and increased caspase-3 activity and annexin V binding of phosphatidylserine. However, simultaneous treatment with SIN (10, 50 or 250 mu M) inhibited the GAG release and the activity and mRNA expression of MMP-13, and enhanced the activity and mRNA expression of TIMP-1 in a dose-dependent manner in cartilage explants. Furthermore, DNA fragment, caspase-3 activity and apoptosis rate were down-regulated, and cell viability was up-regulated dose-dependently in chondrocytes. Thus, SIN has the protective capacity to antagonize cartilage degradation and chondrocyte apoptosis, which suggest that SIN may act as an agent for pharmacological intervention in the progress of OA.

语种: 英语
所属项目编号: 30973043 ; 30700906 ; 7102159 ; 20070001784
项目资助者: National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation ; Ministry of Education of China
WOS记录号: WOS:000280377800006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62310
Appears in Collections:北京大学第三临床医学院_神经内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Neurol, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China

Recommended Citation:
Ju, Xiao-dong,Deng, Min,Ao, Ying-fang,et al. Protective Effect of Sinomenine on Cartilage Degradation and Chondrocytes Apoptosis[J]. YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN,2010,130(8):1053-1060.
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